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Strain Name:
C57BL/6J-MtgxR4858Btlr/Mmmh
Stock Number:
042469-MU
Citation ID:
RRID:MMRRC_042469-MU
Other Names:
R4858 (G1), C57BL/6J-MtgxR4858Btlr
Major Collection:

Strain Information

Sulf2
Name: sulfatase 2
Synonyms: 2010004N24Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 72043
Homologene: 10313
Setd5
Name: SET domain containing 5
Synonyms: 2900045N06Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 72895
Homologene: 12485
Tjp2
Name: tight junction protein 2
Synonyms: ZO-2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 21873
VEGA: 19
Homologene: 3541
Tanc1
Name: tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 1
Synonyms: 1200003E16Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 66860
Homologene: 18946
Trim6
Name: tripartite motif-containing 6
Synonyms: D7Ertd684e, C430046K18Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 94088
Homologene: 14381
Mtor
Name: mechanistic target of rapamycin kinase
Synonyms: FKBP-rapamycin-associated protein FRAP, 2610315D21Rik, RAPT1, RAFT1, flat, Frap1, mechanistic target of rapamycin (serine/threonine kinase)
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 56717
HGNC: HGNC:3942
Homologene: 3637
Ankrd12
Name: ankyrin repeat domain 12
Synonyms: ANCO-2, GAC-1, 2900001A12Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 106585
Homologene: 9059
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 58,104,481 bp
  • C to T, chromosome 1 at 75,243,554 bp
  • G to T, chromosome 1 at 75,421,735 bp
  • T to C, chromosome 1 at 75,555,085 bp
  • T to C, chromosome 1 at 118,669,509 bp
  • A to T, chromosome 1 at 174,250,696 bp
  • A to G, chromosome 1 at 190,800,318 bp
  • A to G, chromosome 1 at 193,113,764 bp
  • C to T, chromosome 2 at 59,724,668 bp
  • T to A, chromosome 2 at 59,907,743 bp
  • T to G, chromosome 2 at 86,978,349 bp
  • A to T, chromosome 2 at 121,098,964 bp
  • C to A, chromosome 2 at 166,081,604 bp
  • A to C, chromosome 3 at 28,638,388 bp
  • C to A, chromosome 3 at 100,967,053 bp
  • G to A, chromosome 3 at 122,546,315 bp
  • A to T, chromosome 3 at 154,786,808 bp
  • T to G, chromosome 4 at 45,923,244 bp
  • T to C, chromosome 4 at 59,612,812 bp
  • T to C, chromosome 4 at 148,454,816 bp
  • A to T, chromosome 5 at 18,291,598 bp
  • G to T, chromosome 5 at 21,950,857 bp
  • T to C, chromosome 5 at 67,417,572 bp
  • G to A, chromosome 5 at 136,743,058 bp
  • T to C, chromosome 5 at 143,373,446 bp
  • A to T, chromosome 5 at 144,012,810 bp
  • T to C, chromosome 5 at 150,401,643 bp
  • T to C, chromosome 6 at 70,704,044 bp
  • C to T, chromosome 6 at 113,149,566 bp
  • T to C, chromosome 6 at 113,317,678 bp
  • T to A, chromosome 7 at 12,493,056 bp
  • A to T, chromosome 7 at 18,353,484 bp
  • C to A, chromosome 7 at 28,988,770 bp
  • A to G, chromosome 7 at 38,099,319 bp
  • T to G, chromosome 7 at 41,218,161 bp
  • T to A, chromosome 7 at 82,671,627 bp
  • A to T, chromosome 7 at 100,454,953 bp
  • T to A, chromosome 7 at 104,232,485 bp
  • C to T, chromosome 7 at 118,543,342 bp
  • T to C, chromosome 7 at 126,110,065 bp
  • G to A, chromosome 7 at 140,112,586 bp
  • C to T, chromosome 7 at 143,681,480 bp
  • C to A, chromosome 8 at 13,013,972 bp
  • T to A, chromosome 8 at 18,975,077 bp
  • A to G, chromosome 8 at 62,021,314 bp
  • T to A, chromosome 8 at 70,104,055 bp
  • T to G, chromosome 8 at 119,772,523 bp
  • C to T, chromosome 9 at 5,306,742 bp
  • C to T, chromosome 9 at 48,495,668 bp
  • A to T, chromosome 9 at 109,234,695 bp
  • T to C, chromosome 10 at 10,349,577 bp
  • T to A, chromosome 10 at 24,922,638 bp
  • TTTGCGCATT to TTT, chromosome 10 at 27,043,643 bp
  • G to A, chromosome 10 at 41,233,590 bp
  • G to A, chromosome 10 at 100,494,911 bp
  • A to G, chromosome 11 at 6,274,456 bp
  • T to A, chromosome 11 at 58,501,909 bp
  • T to A, chromosome 11 at 58,841,207 bp
  • C to T, chromosome 11 at 72,961,470 bp
  • T to A, chromosome 11 at 73,776,546 bp
  • A to T, chromosome 11 at 100,132,071 bp
  • A to G, chromosome 11 at 100,550,321 bp
  • C to A, chromosome 11 at 101,331,662 bp
  • C to T, chromosome 11 at 102,440,156 bp
  • G to A, chromosome 12 at 73,545,912 bp
  • T to C, chromosome 12 at 91,260,162 bp
  • G to T, chromosome 14 at 53,616,693 bp
  • T to C, chromosome 14 at 65,075,994 bp
  • T to C, chromosome 14 at 110,751,883 bp
  • A to T, chromosome 15 at 44,491,101 bp
  • T to A, chromosome 15 at 79,122,946 bp
  • G to A, chromosome 15 at 99,819,576 bp
  • T to C, chromosome 16 at 3,592,842 bp
  • A to T, chromosome 17 at 26,216,617 bp
  • A to T, chromosome 17 at 33,997,324 bp
  • G to A, chromosome 17 at 35,637,576 bp
  • T to C, chromosome 17 at 43,303,672 bp
  • A to G, chromosome 17 at 46,430,747 bp
  • A to G, chromosome 17 at 55,592,894 bp
  • T to C, chromosome 17 at 66,031,433 bp
  • A to G, chromosome 17 at 84,600,697 bp
  • A to T, chromosome 18 at 5,213,154 bp
  • T to C, chromosome 18 at 30,344,078 bp
  • T to C, chromosome 18 at 33,176,213 bp
  • A to G, chromosome 18 at 37,692,100 bp
  • T to C, chromosome 18 at 42,523,981 bp
  • T to C, chromosome 18 at 43,334,014 bp
  • A to G, chromosome 19 at 11,301,612 bp
  • C to A, chromosome 19 at 24,122,120 bp
  • A to T, chromosome 19 at 36,605,282 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4858 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042469-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.