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Strain Name:
C57BL/6J-MtgxR4860Btlr/Mmmh
Stock Number:
042471-MU
Citation ID:
RRID:MMRRC_042471-MU
Other Names:
R4860 (G1), C57BL/6J-MtgxR4860Btlr
Major Collection:

Strain Information

Pax3
Name: paired box 3
Synonyms: Pax-3, Splchl2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18505
HGNC: HGNC:8617
Homologene: 22494
Tle4
Name: transducin-like enhancer of split 4
Synonyms: Grg4, ESTM13, ESTM14, Bce1, 5730411M05Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 21888
VEGA: 19
Homologene: 38259
Matr3
Name: matrin 3
Synonyms: D030046F20Rik, 2810017I02Rik, 1110061A14Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 17184
HGNC: HGNC:6912
Homologene: 7830
Limch1
Name: LIM and calponin homology domains 1
Synonyms: 3732412D22Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 77569
Homologene: 18953
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Arid5b
Name: AT-rich interaction domain 5B
Synonyms: 5430435G07Rik, Desrt, Mrf2beta, Mrf2alpha, Mrf2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 71371
VEGA: 10
Homologene: 45872
Lrig3
Name: leucine-rich repeats and immunoglobulin-like domains 3
Synonyms: 9030421L11Rik, 9130004I02Rik, 9430095K15Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 320398
VEGA: 10
Homologene: 62452
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 78,192,456 bp
  • CAGAGAGAGAGAGA to CAGAGAGAGAGA, chromosome 1 at 88,211,984 bp
  • A to G, chromosome 1 at 91,387,383 bp
  • A to T, chromosome 1 at 188,553,275 bp
  • G to A, chromosome 1 at 195,062,396 bp
  • T to C, chromosome 2 at 25,578,753 bp
  • T to C, chromosome 2 at 86,422,957 bp
  • T to G, chromosome 2 at 158,074,935 bp
  • T to C, chromosome 3 at 64,275,601 bp
  • C to A, chromosome 3 at 79,936,674 bp
  • T to A, chromosome 3 at 95,988,993 bp
  • A to G, chromosome 3 at 122,961,363 bp
  • A to T, chromosome 4 at 19,425,569 bp
  • G to T, chromosome 4 at 40,210,000 bp
  • A to G, chromosome 4 at 56,899,164 bp
  • C to T, chromosome 4 at 83,464,897 bp
  • A to C, chromosome 4 at 101,789,337 bp
  • A to C, chromosome 4 at 109,742,896 bp
  • A to G, chromosome 4 at 115,038,474 bp
  • A to T, chromosome 4 at 123,486,750 bp
  • A to G, chromosome 4 at 133,579,959 bp
  • A to G, chromosome 4 at 145,087,161 bp
  • G to T, chromosome 4 at 152,138,846 bp
  • G to A, chromosome 5 at 4,034,916 bp
  • A to T, chromosome 5 at 5,736,589 bp
  • A to G, chromosome 5 at 21,901,751 bp
  • A to G, chromosome 5 at 66,961,186 bp
  • G to T, chromosome 5 at 92,943,086 bp
  • T to C, chromosome 5 at 102,990,619 bp
  • T to A, chromosome 5 at 121,305,818 bp
  • T to C, chromosome 6 at 24,450,842 bp
  • G to A, chromosome 6 at 37,564,491 bp
  • G to T, chromosome 6 at 47,517,146 bp
  • T to A, chromosome 6 at 47,517,191 bp
  • A to T, chromosome 6 at 115,848,926 bp
  • G to A, chromosome 6 at 125,030,930 bp
  • A to G, chromosome 6 at 137,514,295 bp
  • A to G, chromosome 6 at 146,990,514 bp
  • A to G, chromosome 7 at 4,276,669 bp
  • C to T, chromosome 7 at 4,757,469 bp
  • T to A, chromosome 7 at 12,152,756 bp
  • T to C, chromosome 7 at 35,643,710 bp
  • T to G, chromosome 7 at 41,218,161 bp
  • T to C, chromosome 7 at 75,867,050 bp
  • T to C, chromosome 7 at 81,495,720 bp
  • T to A, chromosome 7 at 106,082,152 bp
  • A to T, chromosome 7 at 106,163,436 bp
  • G to A, chromosome 7 at 116,340,156 bp
  • A to T, chromosome 7 at 130,533,591 bp
  • G to A, chromosome 7 at 142,211,908 bp
  • A to G, chromosome 7 at 143,840,500 bp
  • T to C, chromosome 8 at 12,649,722 bp
  • C to T, chromosome 8 at 71,710,481 bp
  • T to C, chromosome 8 at 72,455,423 bp
  • T to C, chromosome 8 at 105,567,368 bp
  • T to G, chromosome 8 at 105,976,189 bp
  • C to T, chromosome 9 at 21,309,674 bp
  • G to T, chromosome 9 at 39,942,504 bp
  • A to G, chromosome 9 at 60,737,810 bp
  • C to T, chromosome 9 at 71,858,840 bp
  • A to G, chromosome 9 at 90,054,548 bp
  • A to G, chromosome 9 at 107,655,064 bp
  • G to A, chromosome 9 at 110,635,194 bp
  • G to A, chromosome 9 at 121,250,902 bp
  • G to T, chromosome 10 at 68,243,095 bp
  • T to A, chromosome 10 at 80,027,066 bp
  • C to T, chromosome 10 at 111,985,691 bp
  • C to A, chromosome 10 at 126,011,052 bp
  • C to T, chromosome 10 at 127,553,824 bp
  • T to A, chromosome 10 at 130,385,843 bp
  • C to T, chromosome 11 at 50,864,612 bp
  • G to A, chromosome 11 at 55,399,211 bp
  • A to T, chromosome 11 at 61,452,744 bp
  • T to A, chromosome 11 at 68,544,053 bp
  • A to G, chromosome 11 at 70,611,592 bp
  • C to T, chromosome 11 at 84,893,353 bp
  • C to A, chromosome 11 at 87,794,534 bp
  • T to A, chromosome 11 at 87,889,225 bp
  • T to C, chromosome 11 at 115,913,504 bp
  • A to T, chromosome 12 at 80,340,232 bp
  • A to G, chromosome 12 at 91,831,602 bp
  • A to T, chromosome 12 at 102,744,055 bp
  • T to A, chromosome 13 at 14,019,795 bp
  • A to G, chromosome 13 at 97,155,540 bp
  • G to T, chromosome 13 at 113,045,508 bp
  • A to T, chromosome 14 at 26,460,209 bp
  • A to G, chromosome 14 at 32,863,785 bp
  • T to C, chromosome 14 at 55,781,927 bp
  • G to A, chromosome 14 at 56,082,280 bp
  • T to C, chromosome 14 at 70,777,516 bp
  • T to C, chromosome 14 at 110,751,883 bp
  • G to T, chromosome 15 at 5,242,606 bp
  • A to G, chromosome 15 at 30,881,167 bp
  • T to C, chromosome 15 at 44,537,378 bp
  • G to A, chromosome 16 at 17,777,016 bp
  • A to G, chromosome 16 at 18,310,901 bp
  • A to T, chromosome 16 at 31,103,499 bp
  • G to A, chromosome 16 at 32,754,616 bp
  • T to A, chromosome 16 at 32,754,625 bp
  • A to G, chromosome 16 at 38,828,145 bp
  • C to T, chromosome 16 at 97,751,536 bp
  • A to C, chromosome 17 at 23,401,803 bp
  • A to G, chromosome 17 at 24,884,456 bp
  • A to G, chromosome 17 at 25,788,700 bp
  • G to T, chromosome 17 at 25,864,647 bp
  • A to T, chromosome 17 at 32,883,340 bp
  • A to G, chromosome 17 at 56,888,655 bp
  • T to A, chromosome 18 at 35,581,640 bp
  • T to A, chromosome 18 at 36,196,547 bp
  • C to T, chromosome 18 at 36,765,963 bp
  • G to A, chromosome 18 at 60,916,651 bp
  • A to T, chromosome 19 at 14,464,345 bp
  • T to C, chromosome 19 at 40,337,005 bp
  • T to C, chromosome 19 at 57,079,866 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4860 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042471-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.