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Strain Name:
C57BL/6J-MtgxR4868Btlr/Mmmh
Stock Number:
042478-MU
Citation ID:
RRID:MMRRC_042478-MU
Other Names:
R4868 (G1), C57BL/6J-MtgxR4868Btlr
Major Collection:

Strain Information

Mbtps1
Name: membrane-bound transcription factor peptidase, site 1
Synonyms: subtilisin/kexin isozyme-1, SKI-1, S1P, 0610038M03Rik, site-1 protease
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 56453
Homologene: 2808
Ctdspl2
Name: CTD small phosphatase like 2
Synonyms: D2Ertd485e, SCP4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 329506
Homologene: 32311
Itsn1
Name: intersectin 1 (SH3 domain protein 1A)
Synonyms: Ese1, EHSH1, Sh3p17, Eh domain, SH3 domain regulator of endocytosis 1, Intersectin-L
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 16443
HGNC: HGNC:6183
Homologene: 2277
Timeless
Name: timeless circadian clock 1
Synonyms: tim
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 21853
Homologene: 31206
Wdr20
Name: WD repeat domain 20
Synonyms: 2310040A13Rik, Wdr20a
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 69641
Homologene: 32684
Asap1
Name: ArfGAP with SH3 domain, ankyrin repeat and PH domain1
Synonyms: Ddef1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 13196
HGNC: HGNC:2720
Homologene: 7684
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to G, chromosome 1 at 3,216,851 bp
  • T to A, chromosome 1 at 59,870,456 bp
  • T to C, chromosome 1 at 160,130,873 bp
  • C to T, chromosome 1 at 171,252,431 bp
  • T to C, chromosome 1 at 175,663,561 bp
  • T to C, chromosome 2 at 30,956,577 bp
  • G to A, chromosome 2 at 32,692,661 bp
  • C to A, chromosome 2 at 59,924,882 bp
  • T to G, chromosome 2 at 85,776,626 bp
  • T to C, chromosome 2 at 104,255,409 bp
  • T to A, chromosome 2 at 112,156,571 bp
  • C to A, chromosome 2 at 121,993,398 bp
  • T to A, chromosome 3 at 32,952,490 bp
  • C to A, chromosome 3 at 52,103,924 bp
  • T to G, chromosome 3 at 72,943,548 bp
  • T to C, chromosome 3 at 88,399,789 bp
  • A to G, chromosome 3 at 102,997,414 bp
  • A to G, chromosome 3 at 107,600,627 bp
  • G to T, chromosome 3 at 138,483,089 bp
  • T to A, chromosome 4 at 83,694,332 bp
  • T to C, chromosome 4 at 108,549,220 bp
  • T to C, chromosome 4 at 124,751,410 bp
  • A to G, chromosome 4 at 133,486,082 bp
  • T to A, chromosome 4 at 138,395,797 bp
  • T to A, chromosome 4 at 156,009,185 bp
  • T to A, chromosome 5 at 21,748,853 bp
  • A to T, chromosome 5 at 32,829,937 bp
  • T to C, chromosome 5 at 67,324,683 bp
  • A to G, chromosome 5 at 96,083,023 bp
  • T to C, chromosome 5 at 114,398,427 bp
  • A to G, chromosome 5 at 139,170,186 bp
  • A to T, chromosome 5 at 140,629,466 bp
  • C to T, chromosome 5 at 144,833,311 bp
  • C to A, chromosome 6 at 17,819,266 bp
  • A to G, chromosome 6 at 78,381,900 bp
  • T to C, chromosome 6 at 84,179,693 bp
  • C to T, chromosome 6 at 142,416,390 bp
  • T to A, chromosome 7 at 8,481,481 bp
  • T to C, chromosome 7 at 15,627,310 bp
  • T to C, chromosome 7 at 16,199,802 bp
  • T to C, chromosome 7 at 21,133,302 bp
  • T to A, chromosome 7 at 23,504,736 bp
  • T to C, chromosome 7 at 27,517,579 bp
  • A to T, chromosome 7 at 75,743,504 bp
  • C to T, chromosome 7 at 106,971,767 bp
  • T to A, chromosome 7 at 112,318,624 bp
  • G to A, chromosome 8 at 4,247,751 bp
  • A to T, chromosome 8 at 34,092,076 bp
  • A to T, chromosome 8 at 46,634,279 bp
  • A to G, chromosome 8 at 105,710,097 bp
  • C to T, chromosome 8 at 119,508,928 bp
  • T to A, chromosome 9 at 111,365,641 bp
  • A to G, chromosome 9 at 114,492,833 bp
  • C to A, chromosome 9 at 121,583,009 bp
  • A to G, chromosome 10 at 61,862,111 bp
  • T to C, chromosome 10 at 76,178,231 bp
  • G to T, chromosome 10 at 128,247,361 bp
  • A to T, chromosome 11 at 69,463,648 bp
  • T to A, chromosome 11 at 87,853,723 bp
  • T to A, chromosome 11 at 88,074,694 bp
  • A to G, chromosome 11 at 88,844,553 bp
  • G to A, chromosome 11 at 109,974,512 bp
  • G to A, chromosome 11 at 113,803,261 bp
  • G to A, chromosome 11 at 115,429,379 bp
  • A to T, chromosome 11 at 118,108,212 bp
  • A to G, chromosome 11 at 121,831,057 bp
  • T to C, chromosome 12 at 84,370,952 bp
  • T to C, chromosome 12 at 86,062,181 bp
  • T to A, chromosome 12 at 110,738,234 bp
  • T to A, chromosome 13 at 20,914,981 bp
  • T to A, chromosome 13 at 24,464,264 bp
  • A to G, chromosome 13 at 24,694,141 bp
  • A to T, chromosome 14 at 28,956,786 bp
  • T to C, chromosome 14 at 55,773,722 bp
  • C to A, chromosome 14 at 79,183,082 bp
  • A to T, chromosome 14 at 118,877,214 bp
  • G to A, chromosome 15 at 60,015,968 bp
  • A to G, chromosome 15 at 64,094,181 bp
  • C to T, chromosome 15 at 101,568,121 bp
  • T to A, chromosome 16 at 19,701,290 bp
  • T to A, chromosome 16 at 45,104,413 bp
  • T to A, chromosome 16 at 91,785,317 bp
  • C to T, chromosome 17 at 7,351,599 bp
  • C to A, chromosome 17 at 13,728,008 bp
  • A to G, chromosome 17 at 24,901,415 bp
  • G to T, chromosome 17 at 36,548,919 bp
  • G to T, chromosome 17 at 39,074,024 bp
  • A to T, chromosome 17 at 40,633,529 bp
  • A to T, chromosome 17 at 58,974,756 bp
  • T to C, chromosome 18 at 60,695,476 bp
  • A to T, chromosome 18 at 64,551,866 bp
  • A to T, chromosome 19 at 4,864,454 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4868 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042478-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.