Strain Name:
C57BL/6J-MtgxR4889Btlr/Mmmh
Stock Number:
042494-MU
Citation ID:
RRID:MMRRC_042494-MU
Other Names:
R4889 (G1), C57BL/6J-MtgxR4889Btlr
Major Collection:

Strain Information

Sim1
Name: single-minded family bHLH transcription factor 1
Synonyms: bHLHe14
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 20464
VEGA: 10
Homologene: 3715
Card11
Name: caspase recruitment domain family, member 11
Synonyms: 0610008L17Rik, 2410011D02Rik, CARMA1, BIMP3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 108723
Homologene: 13024
Dock1
Name: dedicator of cytokinesis 1
Synonyms: Dock180, 9130006G06Rik, D630004B07Rik, b2b3190Clo
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 330662
HGNC: HGNC:2987
Homologene: 55575
Arhgap21
Name: Rho GTPase activating protein 21
Synonyms: ARHGAP10, 5530401C11Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 71435
Homologene: 10822
Cop1
Name: COP1, E3 ubiquitin ligase
Synonyms: Rfwd2, Cop1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 26374
Homologene: 115565
Dhx9
Name: DExH-box helicase 9
Synonyms: leukophysin, Ddx9, RHA, NDHII, NDH II, nuclear DNA helicase II, RNA helicase
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13211
HGNC: HGNC:2750
Homologene: 1039
Myo18a
Name: myosin XVIIIA
Synonyms: MyoPDZ
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 360013
Homologene: 7977
Akap12
Name: A kinase anchor protein 12
Synonyms: Tsga12, Srcs5, SSeCKS
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 83397
VEGA: 10
HGNC: HGNC:370
Homologene: 3740
Acbd6
Name: acyl-Coenzyme A binding domain containing 6
Synonyms: 2610100E10Rik, 0610010G04Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 72482
Homologene: 12465
Hdc
Name: histidine decarboxylase
Synonyms: L-histidine decarboxylase, Hdc-s, Hdc-c, Hdc-e, Hdc-a
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 15186
HGNC: HGNC:4855
Homologene: 20490
Efemp2
Name: epidermal growth factor-containing fibulin-like extracellular matrix protein 2
Synonyms: fibulin 4, Fbln4, fibulin-4, 0610011K11Rik, MBP1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 58859
HGNC: HGNC:3219
Homologene: 32339
Npat
Name: nuclear protein in the AT region
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 244879
HGNC: HGNC:7896
Homologene: 1888
Sptbn2
Name: spectrin beta, non-erythrocytic 2
Synonyms: Spnb3
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 20743
VEGA: 19
Homologene: 48482
Baz2b
Name: bromodomain adjacent to zinc finger domain, 2B
Synonyms: D2Ertd794e, 5830435C13Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 407823
HGNC: HGNC:963
Homologene: 8394
Sfxn3
Name: sideroflexin 3
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 94280
VEGA: 19
Homologene: 23394
Srcap
Name: Snf2-related CREBBP activator protein
Synonyms: B930091H02Rik, D030022P06Rik, F630004O05Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 100043597
Homologene: 38213
Dnah5
Name: dynein, axonemal, heavy chain 5
Synonyms: b2b3491Clo, Mdnah5, b2b1537Clo, b2b1134Clo, Dnahc5, b2b1154Clo, b2b1565Clo
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 110082
HGNC: HGNC:2950
Homologene: 1048
Cercam
Name: cerebral endothelial cell adhesion molecule
Synonyms: CerCAM, Ceecam1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 99151
Homologene: 22954
Plcz1
Name: phospholipase C, zeta 1
Synonyms: 1700041H07Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 114875
Homologene: 23815
Ado
Name: 2-aminoethanethiol dioxygenase
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 211488
VEGA: 10
Homologene: 13121
Ppfia4
Name: protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 4
Synonyms: Liprin-alpha4, LOC100042382, Gm3812, 1110008G13Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 68507
HGNC: HGNC:9248
Homologene: 66200
Ankhd1
Name: ankyrin repeat and KH domain containing 1
Synonyms: 1110004O12Rik, 9130019P20Rik, 4933432B13Rik, A530027J04Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 108857
Homologene: 87006
Cntn4
Name: contactin 4
Synonyms: Axcam, BIG-2A
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 269784
HGNC: HGNC:2174
Homologene: 14257
Gnptab
Name: N-acetylglucosamine-1-phosphate transferase, alpha and beta subunits
Synonyms: EG432486
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 432486
Homologene: 32576
Syt16
Name: synaptotagmin XVI
Synonyms: syt14r, Strep14, Syt14l
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 238266
VEGA: 12
Homologene: 12902
Slco1b2
Name: solute carrier organic anion transporter family, member 1b2
Synonyms: Oatp1b2, Slc21a10, mlst-1, Slc21a6, 7330442B20Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 28253
Homologene: 75119
Pde6c
Name: phosphodiesterase 6C, cGMP specific, cone, alpha prime
Synonyms: cpfl1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 110855
VEGA: 19
HGNC: HGNC:8787
Homologene: 4521
Ofcc1
Name: orofacial cleft 1 candidate 1
Synonyms: ojoplano, Opo
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218165
VEGA: 13
Homologene: 125376
Or10ag53
Name: olfactory receptor family 10 subfamily AG member 53
Synonyms: MOR273-4P, Olfr1530-ps1, MOR273-4P, MOR264-20, Olfr1115, GA_x6K02T2Q125-48736906-48737886
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258294
Homologene: 27114
Pde7b
Name: phosphodiesterase 7B
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 29863
HGNC: HGNC:8792
Homologene: 32194
Smarca5
Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5
Synonyms: D330027N15Rik, 4933427E24Rik, Snf2h, MommeD4, D030040M08Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 93762
Homologene: 55764
Or5m12
Name: olfactory receptor family 5 subfamily M member 12
Synonyms: GA_x6K02T2Q125-47384320-47383337, Olfr1024, MOR197-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 257900
Homologene: 128271
Vill
Name: villin-like
Synonyms: Villp
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 22351
Homologene: 22650
Zfp974
Name: zinc finger protein 974
Synonyms: 1700049G17Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 73430
Slc5a9
Name: solute carrier family 5 (sodium/glucose cotransporter), member 9
Synonyms: SGLT4
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230612
Homologene: 17081
Cr2
Name: complement receptor 2
Synonyms: Cr-1, Cr1, CD35, CD21, Cr-2, C3DR
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12902
HGNC: HGNC:2336
Homologene: 55611
Itga3
Name: integrin alpha 3
Synonyms: VLA-3 alpha 3, alpha3-integrin
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16400
HGNC: HGNC:6139
Homologene: 21129
Nkx3-1
Name: NK3 homeobox 1
Synonyms: NKX3.1, bagpipe, Bax, Nkx-3.1, NKX3A
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 18095
HGNC: HGNC:7838
Homologene: 4494
Appl2
Name: adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2
Synonyms: Dip3b
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216190
Homologene: 10046
Ctsm
Name: cathepsin M
Synonyms: 1600027J17Rik, Cat M, Catm
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 64139
VEGA: 13
Homologene: 75181
Or1l4
Name: olfactory receptor family 1 subfamily L member 4
Synonyms: MOR138-1, Olfr365, GA_x6K02T2NLDC-33885305-33886243
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258656
Homologene: 74156
Vamp8
Name: vesicle-associated membrane protein 8
Synonyms: endobrevin
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 22320
Homologene: 37846
Gm10729
Name: predicted gene 10729
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Tlx1
Name: T cell leukemia, homeobox 1
Synonyms: Hox-11, Hox11
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 21908
HGNC: HGNC:5056
Homologene: 4031
Slc25a31
Name: solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 31
Synonyms: 1700034J06Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 73333
Homologene: 69485
Tas2r114
Name: taste receptor, type 2, member 114
Synonyms: mt2r46, Tas2r14, T2R14, mGR14
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 387346
Homologene: 128355
Asmt
Name: acetylserotonin O-methyltransferase
Synonyms: Hiomt
Type: Gene
Species: Mouse
Chromosome: X
NCBI: 107626
VEGA: X
HGNC: HGNC:750
A230020J21Rik
Name: RIKEN cDNA A230020J21 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 100125339
Gm10244
Name: predicted gene 10244
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 621427
Gm16505
Name: predicted gene 16505
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 100039182
VEGA: 13
Gm6457
Name: predicted pseudogene 6457
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 623867
VEGA: 18
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 134,300,514 bp
  • A to T, chromosome 1 at 153,481,149 bp
  • A to G, chromosome 1 at 155,567,648 bp
  • A to T, chromosome 1 at 159,284,589 bp
  • A to G, chromosome 1 at 191,025,567 bp
  • A to G, chromosome 1 at 195,176,585 bp
  • T to C, chromosome 2 at 20,880,468 bp
  • A to G, chromosome 2 at 29,881,833 bp
  • A to G, chromosome 2 at 37,202,045 bp
  • A to T, chromosome 2 at 59,936,726 bp
  • C to T, chromosome 2 at 85,904,748 bp
  • A to T, chromosome 2 at 87,252,647 bp
  • T to G, chromosome 2 at 126,594,133 bp
  • A to G, chromosome 3 at 40,721,545 bp
  • C to T, chromosome 3 at 51,694,510 bp
  • C to A, chromosome 4 at 111,891,744 bp
  • T to C, chromosome 5 at 140,885,945 bp
  • A to T, chromosome 6 at 39,420,005 bp
  • G to A, chromosome 6 at 72,385,539 bp
  • A to G, chromosome 6 at 106,525,387 bp
  • A to T, chromosome 6 at 131,689,795 bp
  • T to C, chromosome 6 at 140,007,748 bp
  • T to G, chromosome 6 at 141,656,743 bp
  • A to G, chromosome 7 at 27,910,819 bp
  • T to C, chromosome 7 at 127,538,547 bp
  • C to A, chromosome 7 at 134,744,976 bp
  • C to A, chromosome 8 at 80,704,697 bp
  • T to C, chromosome 9 at 53,562,207 bp
  • T to C, chromosome 9 at 119,063,341 bp
  • C to A, chromosome 10 at 4,356,535 bp
  • T to C, chromosome 10 at 20,548,077 bp
  • A to T, chromosome 10 at 50,981,324 bp
  • G to C, chromosome 10 at 67,548,305 bp
  • G to A, chromosome 10 at 83,641,058 bp
  • A to G, chromosome 10 at 88,433,913 bp
  • T to C, chromosome 11 at 77,832,412 bp
  • T to C, chromosome 11 at 95,068,301 bp
  • A to G, chromosome 12 at 74,129,495 bp
  • A to T, chromosome 13 at 3,361,125 bp
  • G to A, chromosome 13 at 40,015,388 bp
  • A to G, chromosome 13 at 61,538,401 bp
  • G to A, chromosome 14 at 69,190,998 bp
  • G to T, chromosome 15 at 28,235,792 bp
  • A to T, chromosome 18 at 14,570,444 bp
  • A to G, chromosome 18 at 36,578,734 bp
  • T to A, chromosome 19 at 4,729,430 bp
  • T to A, chromosome 19 at 5,475,120 bp
  • T to A, chromosome 19 at 38,133,151 bp
  • T to C, chromosome 19 at 45,049,815 bp
  • G to T, chromosome 19 at 45,150,979 bp
  • G to T, chromosome X at 170,677,029 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4889 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042494-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.