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Strain Name:
C57BL/6J-MtgxR4930Btlr/Mmmh
Stock Number:
042531-MU
Citation ID:
RRID:MMRRC_042531-MU
Other Names:
R4930 (G1), C57BL/6J-MtgxR4930Btlr
Major Collection:

Strain Information

Colgalt2
Name: collagen beta(1-O)galactosyltransferase 2
Synonyms: Glt25d2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 269132
Homologene: 22865
Frem2
Name: Fras1 related extracellular matrix protein 2
Synonyms: 8430406N05Rik, 6030440P17Rik, my, ne, b2b1562Clo
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 242022
Homologene: 18454
Cacna1g
Name: calcium channel, voltage-dependent, T type, alpha 1G subunit
Synonyms: a1G, Cav3.1d
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12291
HGNC: HGNC:1394
Homologene: 22544
P2rx7
Name: purinergic receptor P2X, ligand-gated ion channel, 7
Synonyms: P2X7 receptor, P2X7R, P2X(7)
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18439
HGNC: HGNC:8537
Homologene: 1925
Cops3
Name: COP9 signalosome subunit 3
Synonyms: Sgn3, COP9 complex S3, Csn3
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 26572
HGNC: HGNC:2239
Homologene: 2710
Arid4b
Name: AT-rich interaction domain 4B
Synonyms: RBBP1L1, SAP180, BRCAA1, Rbp1l1, 6720480E17Rik, 6330417L24Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 94246
Homologene: 12847
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 60,366,719 bp
  • A to G, chromosome 1 at 152,499,959 bp
  • T to C, chromosome 1 at 156,320,929 bp
  • C to T, chromosome 1 at 161,241,693 bp
  • C to T, chromosome 1 at 190,808,944 bp
  • G to T, chromosome 2 at 37,783,314 bp
  • T to C, chromosome 2 at 76,732,370 bp
  • T to A, chromosome 2 at 85,849,893 bp
  • A to C, chromosome 2 at 88,124,340 bp
  • G to A, chromosome 2 at 88,391,940 bp
  • C to A, chromosome 2 at 103,091,881 bp
  • C to A, chromosome 2 at 103,266,857 bp
  • A to T, chromosome 2 at 122,151,647 bp
  • A to G, chromosome 2 at 130,564,942 bp
  • T to A, chromosome 2 at 132,546,874 bp
  • A to T, chromosome 2 at 143,749,178 bp
  • A to G, chromosome 2 at 162,965,772 bp
  • G to C, chromosome 2 at 164,199,805 bp
  • A to C, chromosome 3 at 30,607,042 bp
  • A to C, chromosome 3 at 53,656,315 bp
  • T to G, chromosome 3 at 87,728,594 bp
  • A to G, chromosome 3 at 90,212,999 bp
  • C to T, chromosome 3 at 106,164,208 bp
  • T to A, chromosome 4 at 11,615,579 bp
  • A to G, chromosome 4 at 26,369,184 bp
  • A to G, chromosome 4 at 34,044,236 bp
  • A to T, chromosome 4 at 143,369,868 bp
  • T to A, chromosome 5 at 3,661,948 bp
  • G to A, chromosome 5 at 24,400,781 bp
  • T to C, chromosome 5 at 89,439,589 bp
  • A to G, chromosome 5 at 99,222,404 bp
  • T to A, chromosome 5 at 119,883,025 bp
  • C to T, chromosome 5 at 122,670,479 bp
  • A to G, chromosome 6 at 30,630,468 bp
  • A to T, chromosome 6 at 38,391,540 bp
  • T to A, chromosome 6 at 138,153,509 bp
  • G to A, chromosome 7 at 4,460,916 bp
  • A to C, chromosome 7 at 25,917,323 bp
  • A to T, chromosome 7 at 119,951,681 bp
  • C to T, chromosome 8 at 71,630,504 bp
  • T to A, chromosome 8 at 95,097,294 bp
  • G to T, chromosome 8 at 104,447,948 bp
  • T to A, chromosome 8 at 105,272,698 bp
  • A to G, chromosome 8 at 105,617,182 bp
  • T to A, chromosome 9 at 7,447,640 bp
  • T to C, chromosome 9 at 20,260,017 bp
  • T to A, chromosome 9 at 35,215,030 bp
  • T to C, chromosome 9 at 104,011,723 bp
  • A to G, chromosome 9 at 105,300,066 bp
  • A to G, chromosome 9 at 108,087,825 bp
  • C to T, chromosome 10 at 5,052,777 bp
  • A to G, chromosome 10 at 9,760,866 bp
  • T to C, chromosome 10 at 80,155,355 bp
  • A to C, chromosome 10 at 127,041,355 bp
  • C to A, chromosome 11 at 59,835,367 bp
  • T to C, chromosome 11 at 61,453,465 bp
  • A to C, chromosome 11 at 62,118,958 bp
  • G to A, chromosome 11 at 69,354,208 bp
  • T to C, chromosome 11 at 75,451,924 bp
  • T to A, chromosome 11 at 94,444,073 bp
  • A to T, chromosome 11 at 94,503,157 bp
  • T to C, chromosome 11 at 96,318,836 bp
  • A to T, chromosome 11 at 102,690,285 bp
  • T to C, chromosome 11 at 107,620,168 bp
  • T to A, chromosome 12 at 57,673,823 bp
  • C to T, chromosome 12 at 72,906,234 bp
  • T to C, chromosome 12 at 115,920,304 bp
  • A to T, chromosome 13 at 13,510,011 bp
  • T to C, chromosome 13 at 14,187,477 bp
  • T to A, chromosome 13 at 67,294,100 bp
  • T to C, chromosome 13 at 67,366,946 bp
  • T to C, chromosome 13 at 73,268,913 bp
  • G to T, chromosome 13 at 113,327,662 bp
  • A to T, chromosome 14 at 56,534,781 bp
  • A to G, chromosome 14 at 64,032,206 bp
  • A to G, chromosome 14 at 87,141,166 bp
  • C to A, chromosome 15 at 97,965,028 bp
  • T to G, chromosome 16 at 3,592,435 bp
  • C to A, chromosome 16 at 59,314,873 bp
  • A to G, chromosome 17 at 27,193,832 bp
  • A to G, chromosome 17 at 27,718,162 bp
  • A to G, chromosome 17 at 46,510,959 bp
  • A to T, chromosome 18 at 4,349,215 bp
  • T to C, chromosome 19 at 9,010,967 bp
  • A to T, chromosome 19 at 12,627,012 bp
  • T to C, chromosome 19 at 13,795,551 bp
  • T to A, chromosome 19 at 32,052,906 bp
  • A to G, chromosome 19 at 36,115,033 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4930 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042531-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.