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Strain Name:
C57BL/6J-MtgxR4935Btlr/Mmmh
Stock Number:
042535-MU
Citation ID:
RRID:MMRRC_042535-MU
Other Names:
R4935 (G1), C57BL/6J-MtgxR4935Btlr
Major Collection:

Strain Information

Ank2
Name: ankyrin 2, brain
Synonyms: ankyrin B, Ank-2, Ankyrin-2, Ankyrin-B, Gm4392
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 109676
HGNC: HGNC:493
Angpt2
Name: angiopoietin 2
Synonyms: Ang2, Ang-2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11601
HGNC: HGNC:485
Homologene: 22401
Oxr1
Name: oxidation resistance 1
Synonyms: C7B, C7, 2210416C20Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 170719
VEGA: 15
Homologene: 24993
Adcyap1
Name: adenylate cyclase activating polypeptide 1
Synonyms: PACAP
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 11516
VEGA: 17
HGNC: HGNC:241
Homologene: 869
Ptpn3
Name: protein tyrosine phosphatase, non-receptor type 3
Synonyms: PTPCL, 9530011I20Rik, PTP-H1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 545622
HGNC: HGNC:9655
Homologene: 74451
Frmd5
Name: FERM domain containing 5
Synonyms: 1500032A09Rik, A930004K21Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228564
Homologene: 18206
Usp48
Name: ubiquitin specific peptidase 48
Synonyms: D330022K21Rik, 2810449C13Rik, Usp31
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 170707
Homologene: 12988
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 87,767,042 bp
  • T to A, chromosome 1 at 93,395,314 bp
  • T to A, chromosome 2 at 28,024,742 bp
  • G to A, chromosome 2 at 34,704,492 bp
  • T to C, chromosome 2 at 36,927,701 bp
  • T to G, chromosome 2 at 41,498,393 bp
  • G to A, chromosome 2 at 111,527,448 bp
  • T to C, chromosome 2 at 118,713,136 bp
  • T to A, chromosome 2 at 118,718,915 bp
  • A to G, chromosome 2 at 119,068,957 bp
  • A to G, chromosome 2 at 121,562,924 bp
  • A to G, chromosome 2 at 165,517,658 bp
  • T to C, chromosome 2 at 180,101,268 bp
  • T to C, chromosome 2 at 180,173,960 bp
  • T to C, chromosome 3 at 38,949,503 bp
  • A to T, chromosome 3 at 94,937,958 bp
  • T to C, chromosome 3 at 97,171,744 bp
  • T to C, chromosome 3 at 97,662,355 bp
  • A to G, chromosome 3 at 126,956,064 bp
  • T to C, chromosome 4 at 41,394,939 bp
  • A to T, chromosome 4 at 57,197,568 bp
  • A to C, chromosome 4 at 101,792,055 bp
  • G to A, chromosome 4 at 108,007,561 bp
  • C to A, chromosome 4 at 137,650,358 bp
  • T to A, chromosome 5 at 8,737,773 bp
  • T to C, chromosome 5 at 77,037,353 bp
  • T to C, chromosome 5 at 120,486,721 bp
  • G to A, chromosome 5 at 150,051,632 bp
  • A to T, chromosome 6 at 39,023,047 bp
  • T to A, chromosome 6 at 136,808,683 bp
  • A to T, chromosome 7 at 24,466,716 bp
  • T to C, chromosome 7 at 58,813,764 bp
  • A to T, chromosome 7 at 120,016,477 bp
  • T to C, chromosome 8 at 18,692,115 bp
  • C to T, chromosome 8 at 71,399,802 bp
  • G to A, chromosome 8 at 92,855,438 bp
  • T to G, chromosome 8 at 108,947,850 bp
  • A to G, chromosome 9 at 25,306,172 bp
  • T to A, chromosome 9 at 50,771,943 bp
  • A to G, chromosome 9 at 75,445,877 bp
  • A to G, chromosome 9 at 108,487,501 bp
  • A to G, chromosome 10 at 49,240,730 bp
  • T to A, chromosome 10 at 69,976,203 bp
  • A to G, chromosome 10 at 112,272,228 bp
  • A to T, chromosome 11 at 5,283,422 bp
  • A to G, chromosome 11 at 6,200,442 bp
  • T to G, chromosome 11 at 58,188,118 bp
  • A to G, chromosome 11 at 120,377,091 bp
  • A to G, chromosome 13 at 55,093,185 bp
  • A to T, chromosome 13 at 118,386,895 bp
  • T to A, chromosome 14 at 14,100,401 bp
  • A to G, chromosome 14 at 63,381,262 bp
  • T to C, chromosome 15 at 34,428,685 bp
  • T to C, chromosome 15 at 41,813,584 bp
  • T to C, chromosome 15 at 48,161,084 bp
  • A to T, chromosome 17 at 13,890,966 bp
  • A to G, chromosome 17 at 33,925,622 bp
  • A to C, chromosome 17 at 34,023,042 bp
  • A to G, chromosome 17 at 34,547,603 bp
  • A to C, chromosome 17 at 43,295,239 bp
  • A to T, chromosome 17 at 93,204,113 bp
  • G to A, chromosome 18 at 22,523,312 bp
  • T to C, chromosome 19 at 13,619,702 bp
  • A to G, chromosome 19 at 27,322,932 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4935 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042535-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.