Strain Name:
C57BL/6J-MtgxR5020Btlr/Mmmh
Stock Number:
042611-MU
Citation ID:
RRID:MMRRC_042611-MU
Other Names:
R5020 (G1), C57BL/6J-MtgxR5020Btlr
Major Collection:

Strain Information

Lrig2
Name: leucine-rich repeats and immunoglobulin-like domains 2
Synonyms: 4632419I10Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 269473
Homologene: 8882
Mga
Name: MAX gene associated
Synonyms: Mga, D030062C11Rik, C130042M01Rik, Mad5
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 29808
Homologene: 49351
Phf12
Name: PHD finger protein 12
Synonyms: PF1, 2410142K10Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 268448
Homologene: 10841
Rtel1
Name: regulator of telomere elongation helicase 1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 269400
Homologene: 13168
Crybg3
Name: beta-gamma crystallin domain containing 3
Synonyms: Gm9581
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224273
Homologene: 28544
Odf2l
Name: outer dense fiber of sperm tails 2-like
Synonyms: 4733401D09Rik, 9630045K08Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 52184
Homologene: 12011
Map4
Name: microtubule-associated protein 4
Synonyms: MAP 4, Mtap4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17758
HGNC: HGNC:6862
Homologene: 1780
Arid2
Name: AT-rich interaction domain 2
Synonyms: zipzap/p200, 1700124K17Rik, 4432409D24Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 77044
Homologene: 14601
Mettl25
Name: methyltransferase like 25
Synonyms: BC067068
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216292
Homologene: 32774
Rbmxl2
Name: RNA binding motif protein, X-linked-like 2
Synonyms: 1700012H05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 76572
Homologene: 113740
Son
Name: Son DNA binding protein
Synonyms: 2900011L12Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 20658
Homologene: 10551
Ccser2
Name: coiled-coil serine rich 2
Synonyms: 1700012P13Rik, Gcap14, 2900054P12Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 72972
Homologene: 10367
Atp5f1b
Name: ATP synthase F1 subunit beta
Synonyms: Atp5b
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 11947
HGNC: HGNC:830
Homologene: 1273
Zfp219
Name: zinc finger protein 219
Synonyms: 2010302A17Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 69890
VEGA: 14
Homologene: 9504
Spag9
Name: sperm associated antigen 9
Synonyms: 3110018C07Rik, Mapk8ip4, 4831406C20Rik, syd1, JLP, 4733401I23Rik, JIP4
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 70834
Homologene: 2954
Gm14496
Name: predicted gene 14496
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 672125
Homologene: 129606
Numbl
Name: numb-like
Synonyms: nbl
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18223
HGNC: HGNC:8061
Homologene: 68413
Itih5
Name: inter-alpha-trypsin inhibitor, heavy chain 5
Synonyms: 5430408M01Rik, 4631408O11Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 209378
Homologene: 57115
Brca2
Name: breast cancer 2, early onset
Synonyms: RAB163, Fancd1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 12190
HGNC: HGNC:1101
Homologene: 41
Bcl7b
Name: B cell CLL/lymphoma 7B
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 12054
HGNC: HGNC:1005
Homologene: 1290
Actl6a
Name: actin-like 6A
Synonyms: Actl6, Baf53a, 2810432C06Rik, ARP4
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 56456
Homologene: 55811
Tsc1
Name: TSC complex subunit 1
Synonyms: tuberous sclerosis 1, hamartin
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 64930
Homologene: 314
Eif5b
Name: eukaryotic translation initiation factor 5B
Synonyms: IF2, A030003E17Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226982
Homologene: 134613
Mecom
Name: MDS1 and EVI1 complex locus
Synonyms: Evi-1, Jbo, ZNFPR1B1, D630039M04Rik, Prdm3, Mds1, Evi1, MDS1-EVI1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14013
HGNC: HGNC:3498
Homologene: 21086
Nbas
Name: neuroblastoma amplified sequence
Synonyms: 4933425L03Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 71169
VEGA: 12
Homologene: 41073
Anpep
Name: alanyl aminopeptidase, membrane
Synonyms: Apn, aminopeptidase N, Cd13
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 16790
HGNC: HGNC:500
Homologene: 68163
Cdh23
Name: cadherin related 23 (otocadherin)
Synonyms: nmf112, 4930542A03Rik, USH1D, sals, ahl, bob, mdfw, nmf252, nmf181
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 22295
Homologene: 11142
Usf3
Name: upstream transcription factor family member 3
Synonyms: LOC207806, Gm608, LOC385650, 5530400K22Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 207806
Homologene: 19504
Reln
Name: reelin
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 19699
HGNC: HGNC:9957
Homologene: 3699
Uba7
Name: ubiquitin-like modifier activating enzyme 7
Synonyms: Ube1l, 1300004C08Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74153
Homologene: 2502
Wdfy4
Name: WD repeat and FYVE domain containing 4
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 545030
Homologene: 83473
Ankrd34c
Name: ankyrin repeat domain 34C
Synonyms: B230218L05Rik, LOC330998
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 330998
Homologene: 19814
Stxbp5
Name: syntaxin binding protein 5 (tomosyn)
Synonyms: 4930565N16Rik, LGL3, 0710001E20Rik, tomosyn 1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 78808
Homologene: 16402
Nlrp2
Name: NLR family, pyrin domain containing 2
Synonyms: E330007A02Rik, Nalp2, Pan1, Nbs1, PYPAF2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 232827
Homologene: 56789
Mmp3
Name: matrix metallopeptidase 3
Synonyms: stromelysin-1, stromelysin 1, progelatinase, Stmy1, STR-1, Str1, SLN1, SLN-1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17392
VEGA: 9
HGNC: HGNC:7173
Homologene: 20545
Grin2b
Name: glutamate receptor, ionotropic, NMDA2B (epsilon 2)
Synonyms: NMDAR2B, Nmdar2b, GluN2B, NR2B, GluRepsilon2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14812
HGNC: HGNC:4586
Homologene: 646
Spsb3
Name: splA/ryanodine receptor domain and SOCS box containing 3
Synonyms: SSB3, 3300001M01Rik, Tce1, 2310012N15Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 79043
Homologene: 12322
Phlpp2
Name: PH domain and leucine rich repeat protein phosphatase 2
Synonyms: Phlppl, C130044A18Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244650
Homologene: 71015
Vsig10l
Name: V-set and immunoglobulin domain containing 10 like
Synonyms: 2210412E05Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 75690
Homologene: 35386
Plch2
Name: phospholipase C, eta 2
Synonyms: A930027K05Rik, PLCeta2, Plcl4
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 269615
Homologene: 85172
Tgfbr3
Name: transforming growth factor, beta receptor III
Synonyms: TBRIII, 1110036H20Rik, betaglycan
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 21814
Homologene: 2436
Alppl2
Name: alkaline phosphatase, placental-like 2
Synonyms: D1Ertd816e, Akp5
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 11650
Homologene: 129600
Inhca
Name: inhibitor of carbonic anhydrase
Synonyms: mICA, 1300017J02Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 71775
Homologene: 826
Or6p1
Name: olfactory receptor family 6 subfamily P member 1
Synonyms: Olfr414, MOR103-10, GA_x6K02T2P20D-20749615-20748662
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 258756
Homologene: 17383
Arhgap31
Name: Rho GTPase activating protein 31
Synonyms: CdGAP
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 12549
Homologene: 10644
Or4g16
Name: olfactory receptor family 4 subfamily G member 16
Synonyms: MOR245-12, GA_x6K02T2Q125-72357646-72358581, Olfr1279
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258388
Homologene: 121520
Vmn1r184
Name: vomeronasal 1 receptor, 184
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 100312477
Homologene: 74353
Cpxm1
Name: carboxypeptidase X, M14 family member 1
Synonyms: Cpx-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 56264
Homologene: 10485
Zfp946
Name: zinc finger protein 946
Synonyms: 1300003B13Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 74149
Tcerg1l
Name: transcription elongation regulator 1-like
Synonyms: 5730476P14Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 70571
Homologene: 52165
Ikbke
Name: inhibitor of kappaB kinase epsilon
Synonyms: IKKepsilon, IKK-i
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 56489
Homologene: 23168
Clec2i
Name: C-type lectin domain family 2, member i
Synonyms: Dcl1, Clrg, OCILrP2, Clr-g
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 93675
Homologene: 136309
P4ha2
Name: procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha II polypeptide
Synonyms: P4hl
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18452
HGNC: HGNC:8547
Homologene: 55806
Acadsb
Name: acyl-Coenzyme A dehydrogenase, short/branched chain
Synonyms: 1300003O09Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 66885
HGNC: HGNC:91
Homologene: 1216
Pcsk4
Name: proprotein convertase subtilisin/kexin type 4
Synonyms: PC4, SPC5
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 18551
HGNC: HGNC:8746
Homologene: 22495
Mmp24
Name: matrix metallopeptidase 24
Synonyms: MT5-MMP, Membrane type 5-MMP
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17391
HGNC: HGNC:7172
Homologene: 21331
Phospho2
Name: phosphatase, orphan 2
Synonyms: 1700048E23Rik, Phos2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 73373
Homologene: 41756
Rasa4
Name: RAS p21 protein activator 4
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 54153
Homologene: 5080
Nedd9
Name: neural precursor cell expressed, developmentally down-regulated gene 9
Synonyms: Cas-L, CasL, HEF1, E230025G09Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 18003
VEGA: 13
HGNC: HGNC:7733
Homologene: 4669
Plekho1
Name: pleckstrin homology domain containing, family O member 1
Synonyms: Jza2, JZA-20, CKIP-1, 2810052M02Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 67220
Homologene: 9448
Septin12
Name: septin 12
Synonyms: 4933413B09Rik, Sept12, 1700028G04Rik, Septin12
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 71089
VEGA: 16
Homologene: 69435
Txndc9
Name: thioredoxin domain containing 9
Synonyms: ATP binding protein associated with cell differentiation, Apacd
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 98258
Homologene: 4225
Gm26672
Name: predicted gene, 26672
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Igkv4-80
Name: immunoglobulin kappa variable 4-80
Synonyms: Gm16729
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 545848
Gm8126
Name: predicted gene 8126
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 108168152
Tcstv2c
Name: two cell stage variable group member 2C
Synonyms: Gm20767
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 639910
VEGA: 13
Gm21915
Name: predicted gene, 21915
Type: Gene
Species: Mouse
Chromosome: 9
Gemin7
Name: gem nuclear organelle associated protein 7
Synonyms: 2400008I04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 69731
Homologene: 11668
Psma4
Name: proteasome subunit alpha 4
Synonyms: C9
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 26441
HGNC: HGNC:9533
Homologene: 2083
Atp5f1d
Name: ATP synthase F1 subunit delta
Synonyms: 1500000I11Rik, 0610008F14Rik, Atp5d
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 66043
HGNC: HGNC:837
Homologene: 37514
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 37,995,712 bp
  • A to T, chromosome 1 at 38,019,069 bp
  • C to T, chromosome 1 at 87,089,709 bp
  • T to A, chromosome 1 at 131,273,660 bp
  • T to G, chromosome 1 at 174,430,671 bp
  • T to A, chromosome 2 at 10,240,504 bp
  • T to A, chromosome 2 at 28,676,519 bp
  • T to A, chromosome 2 at 69,795,979 bp
  • T to C, chromosome 2 at 111,306,292 bp
  • A to T, chromosome 2 at 119,951,173 bp
  • A to T, chromosome 2 at 130,395,977 bp
  • A to G, chromosome 2 at 155,810,284 bp
  • A to G, chromosome 2 at 181,322,514 bp
  • A to G, chromosome 2 at 181,991,359 bp
  • A to G, chromosome 3 at 29,961,106 bp
  • A to G, chromosome 3 at 32,720,507 bp
  • A to G, chromosome 3 at 95,989,539 bp
  • T to C, chromosome 3 at 104,457,901 bp
  • T to C, chromosome 3 at 145,139,786 bp
  • T to G, chromosome 4 at 155,007,083 bp
  • A to T, chromosome 5 at 22,034,638 bp
  • T to C, chromosome 5 at 107,214,970 bp
  • G to A, chromosome 5 at 135,171,163 bp
  • A to C, chromosome 5 at 136,101,299 bp
  • A to T, chromosome 5 at 150,560,436 bp
  • A to C, chromosome 6 at 69,016,665 bp
  • T to A, chromosome 6 at 128,893,658 bp
  • T to C, chromosome 6 at 135,733,407 bp
  • C to T, chromosome 7 at 5,328,077 bp
  • T to A, chromosome 7 at 19,565,423 bp
  • T to A, chromosome 7 at 26,267,530 bp
  • T to C, chromosome 7 at 27,276,835 bp
  • C to A, chromosome 7 at 43,465,317 bp
  • T to A, chromosome 7 at 79,833,727 bp
  • C to T, chromosome 7 at 107,210,207 bp
  • A to T, chromosome 7 at 131,441,200 bp
  • A to G, chromosome 7 at 138,361,877 bp
  • T to A, chromosome 8 at 109,940,082 bp
  • T to A, chromosome 9 at 7,445,984 bp
  • T to C, chromosome 9 at 40,670,670 bp
  • C to A, chromosome 9 at 54,952,772 bp
  • T to C, chromosome 9 at 89,729,706 bp
  • T to C, chromosome 9 at 103,282,502 bp
  • C to T, chromosome 9 at 107,978,914 bp
  • A to G, chromosome 9 at 110,068,800 bp
  • T to C, chromosome 10 at 9,762,922 bp
  • G to T, chromosome 10 at 60,308,032 bp
  • C to A, chromosome 10 at 80,145,429 bp
  • T to C, chromosome 10 at 80,326,035 bp
  • A to G, chromosome 10 at 105,826,207 bp
  • T to A, chromosome 10 at 128,088,416 bp
  • T to C, chromosome 11 at 54,131,190 bp
  • T to C, chromosome 11 at 78,023,796 bp
  • T to A, chromosome 11 at 94,097,786 bp
  • T to A, chromosome 12 at 13,374,712 bp
  • A to G, chromosome 13 at 41,315,794 bp
  • T to C, chromosome 13 at 120,155,116 bp
  • A to G, chromosome 14 at 33,079,935 bp
  • T to C, chromosome 14 at 36,940,177 bp
  • A to C, chromosome 14 at 43,261,569 bp
  • C to T, chromosome 14 at 52,009,655 bp
  • T to A, chromosome 15 at 96,371,988 bp
  • T to C, chromosome 16 at 4,993,756 bp
  • T to C, chromosome 16 at 38,603,076 bp
  • T to A, chromosome 16 at 44,215,526 bp
  • C to T, chromosome 16 at 59,554,796 bp
  • T to A, chromosome 16 at 91,656,375 bp
  • G to T, chromosome 17 at 22,455,603 bp
  • T to G, chromosome 17 at 24,887,062 bp
  • G to A, chromosome 18 at 37,749,470 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5020 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042611-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.