Strain Name:
C57BL/6J-MtgxR5054Btlr/Mmmh
Stock Number:
042644-MU
Citation ID:
RRID:MMRRC_042644-MU
Other Names:
R5054 (G1), C57BL/6J-MtgxR5054Btlr
Major Collection:

Gene Information

Tgfa
Name: transforming growth factor alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 21802
Homologene: 2431
Eif2s2
Name: eukaryotic translation initiation factor 2, subunit 2 (beta)
Synonyms: EIF2B, 38kDa, 2810026E11Rik, D2Ertd303e, EIF2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 67204
HGNC: HGNC:3266
Homologene: 2904
Fzr1
Name: fizzy and cell division cycle 20 related 1
Synonyms: Cdh1, Fyr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 56371
Homologene: 9444
Slc1a6
Name: solute carrier family 1 (high affinity aspartate/glutamate transporter), member 6
Synonyms: EAAT4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 20513
VEGA: 10
Homologene: 21055
Rad51c
Name: RAD51 paralog C
Synonyms: Rad51l2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 114714
HGNC: HGNC:9820
Homologene: 14238
Mtor
Name: mechanistic target of rapamycin kinase
Synonyms: 2610315D21Rik, FKBP-rapamycin-associated protein FRAP, Frap1, RAPT1, RAFT1, mechanistic target of rapamycin (serine/threonine kinase), flat
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 56717
HGNC: HGNC:3942
Homologene: 3637
Mat2b
Name: methionine adenosyltransferase II, beta
Synonyms: MATIIbeta, 2410018D16Rik, 1110064C04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 108645
HGNC: HGNC:6905
Homologene: 8323
Atad5
Name: ATPase family, AAA domain containing 5
Synonyms: C130052G03Rik, LOC237877
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 237877
Homologene: 32611
Kazn
Name: kazrin, periplakin interacting protein
Synonyms: 9030409G11Rik, 2310007B04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 71529
Homologene: 16235
Birc6
Name: baculoviral IAP repeat-containing 6
Synonyms: apollon, D630005A10Rik, A430040A19Rik, Bruce, A430032G04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 12211
Homologene: 7248
Pds5a
Name: PDS5 cohesin associated factor A
Synonyms: 9030416H16Rik, E230024D05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 71521
Homologene: 22877
Mdm2
Name: transformed mouse 3T3 cell double minute 2
Synonyms: 1700007J15Rik, Mdm-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 17246
HGNC: HGNC:6973
Homologene: 1793
Zfp985
Name: zinc finger protein 985
Synonyms: Gm13154
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 433804
Homologene: 133076
Slc12a3
Name: solute carrier family 12, member 3
Synonyms: TSC, NCC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 20497
Homologene: 287
Rims2
Name: regulating synaptic membrane exocytosis 2
Synonyms: 2810036I15Rik, RIM2, Syt3-rs
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 116838
VEGA: 15
Homologene: 81639
Tubb3
Name: tubulin, beta 3 class III
Synonyms: Tuj1, 3200002H15Rik, betaIII-tubulin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 22152
Homologene: 68503
Bcam
Name: basal cell adhesion molecule
Synonyms: 1200005K12Rik, Lu, B-CAM
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 57278
HGNC: HGNC:6722
Homologene: 21149
Tbr1
Name: T-box brain gene 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 21375
Homologene: 4807
Themis
Name: thymocyte selection associated
Synonyms: Tsepa, E430004N04Rik, Gasp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 210757
Homologene: 72287
Dync2h1
Name: dynein cytoplasmic 2 heavy chain 1
Synonyms: 4432416O06Rik, DHC2, b2b414Clo, Dnchc2, m407Asp, m152Asp, D030010H02Rik, D330044F14Rik, DHC1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 110350
HGNC: HGNC:2962
Homologene: 14468
Kif13a
Name: kinesin family member 13A
Synonyms: 4930505I07Rik, N-3 kinesin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 16553
VEGA: 13
Homologene: 22589
Rps10
Name: ribosomal protein S10
Synonyms: 2210402A09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 67097
Homologene: 788
Rnf219
Name: ring finger protein 219
Synonyms: 2610206B13Rik, 2810449K13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 72486
VEGA: 14
Homologene: 11578
Ppp1r12b
Name: protein phosphatase 1, regulatory subunit 12B
Synonyms: 9530009M10Rik, 1810037O03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 329251
HGNC: HGNC:7619
Homologene: 135710
Rundc1
Name: RUN domain containing 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 217201
Homologene: 15095
Pigo
Name: phosphatidylinositol glycan anchor biosynthesis, class O
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 56703
Homologene: 31761
Klra1
Name: killer cell lectin-like receptor, subfamily A, member 1
Synonyms: Ly49A, Ly49o129
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 16627
Homologene: 110821
Dock3
Name: dedicator of cyto-kinesis 3
Synonyms: Moca, PBP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 208869
HGNC: HGNC:2989
Homologene: 21030
Obscn
Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Synonyms: LOC380698, OTTMUSG00000005786
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 380698
Homologene: 70869
Fmnl1
Name: formin-like 1
Synonyms: formin-related gene in leukocytes, 8030453N10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 57778
HGNC: HGNC:1212
Homologene: 135620
Pam
Name: peptidylglycine alpha-amidating monooxygenase
Synonyms: PHM
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 18484
HGNC: HGNC:8596
Homologene: 37369
Rpl22l1
Name: ribosomal protein L22 like 1
Synonyms: 3110001N18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 68028
Homologene: 134718
Kcna2
Name: potassium voltage-gated channel, shaker-related subfamily, member 2
Synonyms: Akr6a4, Mk-2, Kca1-2, Kv1.2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 16490
HGNC: HGNC:6220
Homologene: 21034
Ptar1
Name: protein prenyltransferase alpha subunit repeat containing 1
Synonyms: 2010013L17Rik, 4930428J16Rik, 1700084D09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 72351
VEGA: 19
Homologene: 34254
Vmn1r222
Name: vomeronasal 1 receptor 222
Synonyms: V1rh16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 171274
Homologene: 110880
Adam28
Name: a disintegrin and metallopeptidase domain 28
Synonyms: C130072N01Rik, Dtgn1, D430033C21Rik, MDC-L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 13522
VEGA: 14
HGNC: HGNC:206
Homologene: 40705
Fndc7
Name: fibronectin type III domain containing 7
Synonyms: E230011A21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 320181
Homologene: 18551
Ccdc8
Name: coiled-coil domain containing 8
Synonyms: ENSMUSG00000041117
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 434130
Homologene: 49977
Ogg1
Name: 8-oxoguanine DNA-glycosylase 1
Synonyms: Mmh
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 18294
HGNC: HGNC:8125
Homologene: 1909
Serpini2
Name: serine (or cysteine) peptidase inhibitor, clade I, member 2
Synonyms: 1810006A24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 67931
HGNC: HGNC:8945
Homologene: 21248
Nostrin
Name: nitric oxide synthase trafficker
Synonyms: mDaIP2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 329416
Homologene: 43142
Dytn
Name: dystrotelin
Synonyms: LOC241073
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 241073
Homologene: 104042
Klk14
Name: kallikrein related-peptidase 14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 317653
HGNC: HGNC:6362
Homologene: 69348
Ssrp1
Name: structure specific recognition protein 1
Synonyms: Hmgox, Hmgi-rs3, T160, Hmg1-rs1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 20833
Homologene: 110735
Gpr45
Name: G protein-coupled receptor 45
Synonyms: 9230112G11Rik, PSP24alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 93690
HGNC: HGNC:4503
Homologene: 5228
Kcna7
Name: potassium voltage-gated channel, shaker-related subfamily, member 7
Synonyms: Kv1.7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 16495
HGNC: HGNC:6226
Homologene: 7791
Zfp184
Name: zinc finger protein 184 (Kruppel-like)
Synonyms: 4930500C15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 193452
Homologene: 113602
4921517D22Rik
Name: RIKEN cDNA 4921517D22 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 70900
VEGA: 13
Homologene: 87278
Vmn2r95
Name: vomeronasal 2, receptor 95
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 328759
Homologene: 115024
Ssx2ip
Name: synovial sarcoma, X 2 interacting protein
Synonyms: Adip
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 99167
Homologene: 8522
Zfp444
Name: zinc finger protein 444
Synonyms: 2810031J10Rik, 6230401O10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 72667
Homologene: 10139
Mgat4d
Name: MGAT4 family, member C
Synonyms: 4933434I20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 67555
Homologene: 128538
Adamtsl2
Name: ADAMTS-like 2
Synonyms: A930008K15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 77794
Homologene: 8798
Gmppa
Name: GDP-mannose pyrophosphorylase A
Synonyms: 1810012N01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 69080
Homologene: 8346
Cyp2a5
Name: cytochrome P450, family 2, subfamily a, polypeptide 5
Synonyms: Coh
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 13087
Homologene: 85917
Tlr12
Name: toll-like receptor 12
Synonyms: LOC384059, Tlr11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 384059
Homologene: 135964
Impa2
Name: inositol (myo)-1(or 4)-monophosphatase 2
Synonyms: 2210415D20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 114663
VEGA: 18
HGNC: HGNC:6051
Homologene: 22799
Btbd7
Name: BTB (POZ) domain containing 7
Synonyms: E130118E17Rik, 5730507E09Rik, FUP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 238386
VEGA: 12
Homologene: 34300
Sephs2
Name: selenophosphate synthetase 2
Synonyms: Sps2, Ysg3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 20768
Homologene: 7550
H1f0
Name: H1 histone family, member 0
Synonyms: H1fv, D130017D06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 14958
VEGA: 15
HGNC: HGNC:4714
Homologene: 136788
Serpina16
Name: serine (or cysteine) peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 16
Synonyms: Gm46, LOC194604
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 194604
Homologene: 52303
Hbb-bh1
Name: hemoglobin Z, beta-like embryonic chain
Synonyms: betaH1, beta Hl globin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 15132
HGNC: HGNC:4830
Homologene: 110794
Gm17472
Name: predicted gene, 17472
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
Gm5039
Name: predicted gene 5039
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 266459
VEGA: 12
Tmppe
Name: transmembrane protein with metallophosphoesterase domain
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 100504715
VEGA: 9
Homologene: 62675
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 43,032,649 bp
  • C to A, chromosome 1 at 63,661,159 bp
  • C to A, chromosome 1 at 75,439,371 bp
  • C to A, chromosome 1 at 97,821,917 bp
  • G to T, chromosome 1 at 134,955,733 bp
  • G to A, chromosome 2 at 27,101,720 bp
  • A to T, chromosome 2 at 61,806,002 bp
  • A to T, chromosome 2 at 69,175,713 bp
  • C to A, chromosome 2 at 85,037,966 bp
  • A to C, chromosome 2 at 154,892,670 bp
  • T to G, chromosome 3 at 28,806,836 bp
  • T to A, chromosome 3 at 75,259,477 bp
  • A to T, chromosome 3 at 107,104,340 bp
  • A to G, chromosome 3 at 108,881,347 bp
  • T to C, chromosome 3 at 146,430,917 bp
  • A to T, chromosome 4 at 43,021,337 bp
  • T to A, chromosome 4 at 128,617,270 bp
  • T to C, chromosome 4 at 142,108,646 bp
  • A to T, chromosome 4 at 147,582,981 bp
  • T to A, chromosome 4 at 148,556,855 bp
  • A to G, chromosome 5 at 65,637,814 bp
  • T to C, chromosome 6 at 42,981,004 bp
  • G to C, chromosome 6 at 86,270,082 bp
  • G to A, chromosome 6 at 113,328,365 bp
  • T to A, chromosome 6 at 130,375,284 bp
  • T to A, chromosome 7 at 6,189,793 bp
  • T to C, chromosome 7 at 16,995,045 bp
  • T to A, chromosome 7 at 19,756,860 bp
  • C to G, chromosome 7 at 26,841,104 bp
  • G to A, chromosome 7 at 43,692,077 bp
  • G to A, chromosome 7 at 45,406,591 bp
  • C to T, chromosome 7 at 103,841,856 bp
  • C to A, chromosome 7 at 127,273,392 bp
  • G to A, chromosome 8 at 83,368,208 bp
  • A to G, chromosome 8 at 94,346,351 bp
  • T to C, chromosome 8 at 123,420,868 bp
  • T to C, chromosome 9 at 7,085,007 bp
  • T to C, chromosome 9 at 106,937,906 bp
  • A to G, chromosome 9 at 114,405,958 bp
  • A to G, chromosome 10 at 28,761,237 bp
  • A to G, chromosome 10 at 78,814,602 bp
  • G to A, chromosome 10 at 81,371,419 bp
  • A to G, chromosome 10 at 117,690,351 bp
  • T to A, chromosome 11 at 40,680,042 bp
  • T to C, chromosome 11 at 59,073,617 bp
  • T to A, chromosome 11 at 80,094,676 bp
  • T to C, chromosome 11 at 87,397,754 bp
  • T to C, chromosome 11 at 101,425,141 bp
  • T to C, chromosome 11 at 103,193,491 bp
  • T to C, chromosome 12 at 88,321,301 bp
  • T to C, chromosome 12 at 102,838,212 bp
  • C to T, chromosome 12 at 103,674,930 bp
  • G to T, chromosome 13 at 21,959,282 bp
  • A to G, chromosome 13 at 23,232,731 bp
  • A to G, chromosome 13 at 46,802,646 bp
  • A to G, chromosome 13 at 59,689,501 bp
  • C to T, chromosome 14 at 68,617,715 bp
  • C to T, chromosome 14 at 104,508,030 bp
  • A to T, chromosome 15 at 39,517,869 bp
  • G to A, chromosome 15 at 79,028,773 bp
  • G to T, chromosome 17 at 18,451,446 bp
  • A to G, chromosome 17 at 27,630,480 bp
  • A to G, chromosome 17 at 74,655,325 bp
  • C to A, chromosome 18 at 67,306,727 bp
  • G to T, chromosome 19 at 23,694,365 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5054 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042644-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.