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Strain Name:
C57BL/6J-MtgxR5055Btlr/Mmmh
Stock Number:
042645-MU
Citation ID:
RRID:MMRRC_042645-MU
Other Names:
R5055 (G1), C57BL/6J-MtgxR5055Btlr
Major Collection:

Strain Information

Myh9
Name: myosin, heavy polypeptide 9, non-muscle
Synonyms: D0Jmb2, E030044M24Rik, NMHC II-A, Myhn-1, Myhn1, myosin IIA, Fltn
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 17886
HGNC: HGNC:7579
Homologene: 129835
Map4k5
Name: mitogen-activated protein kinase kinase kinase kinase 5
Synonyms: MAPKKKK5, GCKR, KHS, 4432415E19Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 399510
HGNC: HGNC:6867
Homologene: 38199
Uso1
Name: USO1 vesicle docking factor
Synonyms: transcytosis associated protein p115, TAP, Vdp
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 56041
Homologene: 2754
Epha2
Name: Eph receptor A2
Synonyms: Sek-2, Eck, Sek2, Myk2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 13836
HGNC: HGNC:3386
Homologene: 20929
Nes
Name: nestin
Synonyms: RC2, Marc2, ESTM46, Ifaprc2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18008
HGNC: HGNC:7756
Homologene: 136487
Prdm16
Name: PR domain containing 16
Synonyms: Mel1, 5730557K01Rik, line 27, csp1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 70673
Homologene: 11139
Phf21a
Name: PHD finger protein 21A
Synonyms: 80kDa, Braf35/HDAC complex (Bhc), PFTF1, Bhc80, D030065N23Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 192285
Homologene: 9597
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 14,875,959 bp
  • T to A, chromosome 1 at 36,273,382 bp
  • T to C, chromosome 1 at 64,079,280 bp
  • G to A, chromosome 1 at 130,811,437 bp
  • G to T, chromosome 1 at 134,955,733 bp
  • C to A, chromosome 1 at 135,402,359 bp
  • T to A, chromosome 1 at 182,571,961 bp
  • T to C, chromosome 2 at 85,887,241 bp
  • T to C, chromosome 2 at 92,351,856 bp
  • C to T, chromosome 2 at 112,831,159 bp
  • T to A, chromosome 2 at 118,718,222 bp
  • A to T, chromosome 2 at 154,013,710 bp
  • G to C, chromosome 2 at 155,632,373 bp
  • G to T, chromosome 2 at 181,086,761 bp
  • T to C, chromosome 3 at 27,372,272 bp
  • A to G, chromosome 3 at 87,977,214 bp
  • T to C, chromosome 3 at 89,023,212 bp
  • T to A, chromosome 3 at 93,651,928 bp
  • A to G, chromosome 4 at 6,990,468 bp
  • T to C, chromosome 4 at 102,195,114 bp
  • G to A, chromosome 4 at 117,868,150 bp
  • G to T, chromosome 4 at 124,743,031 bp
  • A to G, chromosome 4 at 141,309,069 bp
  • A to G, chromosome 4 at 141,466,549 bp
  • T to C, chromosome 4 at 154,335,262 bp
  • G to T, chromosome 5 at 65,266,162 bp
  • C to A, chromosome 5 at 81,646,551 bp
  • A to T, chromosome 5 at 92,192,735 bp
  • A to G, chromosome 5 at 112,875,217 bp
  • G to A, chromosome 6 at 48,464,795 bp
  • G to A, chromosome 6 at 55,068,092 bp
  • A to G, chromosome 6 at 66,536,034 bp
  • A to C, chromosome 6 at 70,687,239 bp
  • A to G, chromosome 6 at 125,100,986 bp
  • T to C, chromosome 6 at 130,629,946 bp
  • C to T, chromosome 6 at 141,487,956 bp
  • G to A, chromosome 7 at 45,406,591 bp
  • A to T, chromosome 7 at 73,480,508 bp
  • A to T, chromosome 7 at 76,413,577 bp
  • A to G, chromosome 7 at 81,169,048 bp
  • A to T, chromosome 7 at 81,974,415 bp
  • T to A, chromosome 7 at 106,865,730 bp
  • A to G, chromosome 7 at 133,714,923 bp
  • A to T, chromosome 7 at 139,661,190 bp
  • A to T, chromosome 7 at 143,072,784 bp
  • T to G, chromosome 8 at 39,623,956 bp
  • A to G, chromosome 9 at 44,818,352 bp
  • A to G, chromosome 9 at 55,500,133 bp
  • T to C, chromosome 9 at 55,859,719 bp
  • A to G, chromosome 9 at 114,390,140 bp
  • A to G, chromosome 9 at 119,522,566 bp
  • A to T, chromosome 10 at 24,000,947 bp
  • A to T, chromosome 10 at 39,923,777 bp
  • A to G, chromosome 10 at 79,784,344 bp
  • A to G, chromosome 10 at 83,556,907 bp
  • A to T, chromosome 10 at 107,534,679 bp
  • C to T, chromosome 10 at 117,744,979 bp
  • T to C, chromosome 10 at 123,196,901 bp
  • G to A, chromosome 11 at 46,123,169 bp
  • A to G, chromosome 11 at 69,520,773 bp
  • T to C, chromosome 11 at 95,039,416 bp
  • A to T, chromosome 12 at 32,833,121 bp
  • A to T, chromosome 12 at 69,831,558 bp
  • A to G, chromosome 13 at 53,342,182 bp
  • T to C, chromosome 13 at 61,196,350 bp
  • T to A, chromosome 13 at 95,015,217 bp
  • T to A, chromosome 13 at 100,539,422 bp
  • T to A, chromosome 14 at 21,516,994 bp
  • C to T, chromosome 14 at 54,468,345 bp
  • A to C, chromosome 15 at 6,585,149 bp
  • C to A, chromosome 15 at 26,552,936 bp
  • G to A, chromosome 15 at 77,764,523 bp
  • A to G, chromosome 15 at 89,282,949 bp
  • T to C, chromosome 15 at 95,473,579 bp
  • T to A, chromosome 16 at 35,844,363 bp
  • A to T, chromosome 16 at 96,427,803 bp
  • A to T, chromosome 17 at 30,918,887 bp
  • A to G, chromosome 17 at 32,398,893 bp
  • G to T, chromosome 17 at 33,912,160 bp
  • G to A, chromosome 17 at 34,448,796 bp
  • T to A, chromosome 18 at 80,456,088 bp
  • G to T, chromosome 19 at 46,322,468 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5055 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042645-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.