Strain Name:
C57BL/6J-MtgxR5062Btlr/Mmmh
Stock Number:
042652-MU
Citation ID:
RRID:MMRRC_042652-MU
Other Names:
R5062 (G1), C57BL/6J-MtgxR5062Btlr
Major Collection:

Gene Information

Otx1
Name: orthodenticle homeobox 1
Synonyms: A730044F23Rik, jv
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18423
HGNC: HGNC:8521
Homologene: 7875
Limch1
Name: LIM and calponin homology domains 1
Synonyms: 3732412D22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 77569
Homologene: 18953
Nae1
Name: NEDD8 activating enzyme E1 subunit 1
Synonyms: Appbp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234664
HGNC: HGNC:621
Homologene: 68370
Herc3
Name: hect domain and RLD 3
Synonyms: 5730409F18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 73998
HGNC: HGNC:4876
Homologene: 57095
Spns1
Name: spinster homolog 1
Synonyms: 2210013K02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 73658
Homologene: 41530
Slamf6
Name: SLAM family member 6
Synonyms: SF2000, NTB-A, KAL1b, KAL1, Ly108
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 30925
Homologene: 49945
Fryl
Name: FRY like transcription coactivator
Synonyms: 2310004H21Rik, 2510002A14Rik, 9030227G01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 72313
Homologene: 103956
Pcm1
Name: pericentriolar material 1
Synonyms: 9430077F19Rik, 2600002H09Rik, C030044G17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 18536
HGNC: HGNC:8727
Homologene: 4518
Lcmt1
Name: leucine carboxyl methyltransferase 1
Synonyms: Lcmt, LCMT-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 30949
Homologene: 41123
Nisch
Name: nischarin
Synonyms: 1200007D05Rik, 3202002H23Rik, edsn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 64652
Homologene: 136161
Cdca4
Name: cell division cycle associated 4
Synonyms: 2410018C03Rik, Hepp, SEI-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 71963
VEGA: 12
Homologene: 9936
Pi4ka
Name: phosphatidylinositol 4-kinase alpha
Synonyms: Pik4ca
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 224020
HGNC: HGNC:8983
Homologene: 11171
Hectd1
Name: HECT domain E3 ubiquitin protein ligase 1
Synonyms: A630086P08Rik, opm, b2b327Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 207304
VEGA: 12
Homologene: 9115
Supt5
Name: suppressor of Ty 5, DSIF elongation factor subunit
Synonyms: Spt5, Supt5h
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 20924
Homologene: 2384
Kctd3
Name: potassium channel tetramerisation domain containing 3
Synonyms: NY-REN-45, E330032J19Rik, 4930438A20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226823
Homologene: 9395
Senp3
Name: SUMO/sentrin specific peptidase 3
Synonyms: Smt3ip1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 80886
Homologene: 9236
Mc5r
Name: melanocortin 5 receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 17203
VEGA: 18
HGNC: HGNC:6933
Homologene: 4321
Ryr2
Name: ryanodine receptor 2, cardiac
Synonyms: 9330127I20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 20191
VEGA: 13
Homologene: 37423
Thbs1
Name: thrombospondin 1
Synonyms: TSP1, TSP-1, Thbs-1, tbsp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 21825
Homologene: 31142
Bcam
Name: basal cell adhesion molecule
Synonyms: B-CAM, 1200005K12Rik, Lu
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 57278
HGNC: HGNC:6722
Homologene: 21149
Grm7
Name: glutamate receptor, metabotropic 7
Synonyms: mGluR7, Gpr1g, E130018M02Rik, 6330570A01Rik, mGlu7a receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 108073
HGNC: HGNC:4599
Homologene: 20233
Sun2
Name: Sad1 and UNC84 domain containing 2
Synonyms: B230369L08Rik, Unc84b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223697
Homologene: 9113
Vsir
Name: V-set immunoregulatory receptor
Synonyms: Dies1, VISTA, PD-1H, 4632428N05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 74048
Homologene: 81923
Casp2
Name: caspase 2
Synonyms: Ich-1, Caspase-2, Nedd2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12366
HGNC: HGNC:1503
Homologene: 7254
Nectin2
Name: nectin cell adhesion molecule 2
Synonyms: nectin-2, MPH, Cd112, Pvs, Pvrl2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 19294
HGNC: HGNC:9707
Homologene: 86092
Peak1
Name: pseudopodium-enriched atypical kinase 1
Synonyms: 1110049L02Rik, NKF3 kinase family member, C230081A13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 244895
Homologene: 18259
Asxl3
Name: ASXL transcriptional regulator 3
Synonyms: LOC381127, D930044O18Rik, D430002O22Rik, C230079D11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 211961
Homologene: 19371
Clu
Name: clusterin
Synonyms: testosterone repressed prostate message-2, SP-40, complement lysis inhibitor, Sgp-2, ApoJ, Apolipoprotein J, Cli, Sugp-2, Sgp2, D14Ucla3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 12759
HGNC: HGNC:2095
Homologene: 1382
Umod
Name: uromodulin
Synonyms: Tamm-Horsfall glycoprotein, uromucoid, urehr4, Urehd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 22242
Homologene: 2522
Ncoa1
Name: nuclear receptor coactivator 1
Synonyms: SRC-a/NCoA-1, SRC-1, SRC1, steroid receptor coactivator-1, KAT13A, bHLHe74
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 17977
VEGA: 12
HGNC: HGNC:7668
Homologene: 7859
Atp6v0a4
Name: ATPase, H+ transporting, lysosomal V0 subunit A4
Synonyms: V-ATPase alpha 4, Atp6n1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 140494
HGNC: HGNC:866
Homologene: 39904
Pkhd1
Name: polycystic kidney and hepatic disease 1
Synonyms: tigmin, FPC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 241035
HGNC: HGNC:9016
Homologene: 16336
Lamc3
Name: laminin gamma 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 23928
HGNC: HGNC:6494
Homologene: 21222
Zfp646
Name: zinc finger protein 646
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233905
Homologene: 8802
Sharpin
Name: SHANK-associated RH domain interacting protein
Synonyms: SIPL1, 0610041B22Rik, cpdm
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 106025
Homologene: 11918
Neb
Name: nebulin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 17996
HGNC: HGNC:7720
Homologene: 136285
Col6a3
Name: collagen, type VI, alpha 3
Synonyms: Col6a-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12835
HGNC: HGNC:2213
Homologene: 37917
Mroh2a
Name: maestro heat-like repeat family member 2A
Synonyms: ENSMUSG00000044873, OTTMUSG00000020804, Heatr7b1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 100040766
Homologene: 85300
Ttc21a
Name: tetratricopeptide repeat domain 21A
Synonyms: 4921538N17Rik, Thm2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 74052
Homologene: 14728
Fshr
Name: follicle stimulating hormone receptor
Synonyms: follicle-stimulating hormone receptor, Follitropin receptor, FSH-R
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 14309
VEGA: 17
HGNC: HGNC:3969
Homologene: 117
Abca6
Name: ATP-binding cassette, sub-family A (ABC1), member 6
Synonyms: 6330565N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 76184
HGNC: HGNC:36
Homologene: 71264
Tns1
Name: tensin 1
Synonyms: 1200014E20Rik, E030018G17Rik, 1110018I21Rik, Tns, E030037J05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 21961
Homologene: 11219
Cpne9
Name: copine family member IX
Synonyms: A730016F12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 211232
Homologene: 84686
Fam186a
Name: family with sequence similarity 186, member A
Synonyms: LOC380973, 1700030F18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 72277
Phgdh
Name: 3-phosphoglycerate dehydrogenase
Synonyms: SERA, PGDH, PGD, PGAD, A10, 3-PGDH, 3PGDH
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 236539
HGNC: HGNC:8923
Homologene: 39318
Fscn2
Name: fascin actin-bundling protein 2
Synonyms: C630046B20Rik, ahl8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 238021
HGNC: HGNC:3960
Homologene: 22722
Lrfn2
Name: leucine rich repeat and fibronectin type III domain containing 2
Synonyms: 5730420O05Rik, SALM1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 70530
Homologene: 19182
Nlrp5
Name: NLR family, pyrin domain containing 5
Synonyms: Op1, Mater, Nalp5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 23968
Homologene: 65105
Cyp3a13
Name: cytochrome P450, family 3, subfamily a, polypeptide 13
Synonyms: IIIAm2, steroid inducible
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 13113
HGNC: HGNC:2640
Homologene: 133564
Artn
Name: artemin
Synonyms: neublastin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 11876
HGNC: HGNC:727
Homologene: 7631
Spef1
Name: sperm flagellar 1
Synonyms: 4931426K16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 70997
Homologene: 15741
Pak1ip1
Name: PAK1 interacting protein 1
Synonyms: 5830431I15Rik, 5930415H02Rik, p21-activated protein kinase-interacting protein 1, PIP1, Gdpd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 68083
Homologene: 39574
Klra9
Name: killer cell lectin-like receptor subfamily A, member 9
Synonyms: Ly49I, LY49I1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16640
Homologene: 110821
Glyat
Name: glycine-N-acyltransferase
Synonyms: A330009E03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 107146
Homologene: 64840
Sema4c
Name: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4C
Synonyms: M-Sema F, Semaf, Semacl1, Semai, Semacl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 20353
Homologene: 23047
Klhl30
Name: kelch-like 30
Synonyms: 4631423F02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 70788
Homologene: 18891
Tmem200a
Name: transmembrane protein 200A
Synonyms: C030003D03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 77220
VEGA: 10
Homologene: 14175
Ptgs1
Name: prostaglandin-endoperoxide synthase 1
Synonyms: Cox-1, cyclooxygenase 1, Pghs1, COX1, Cox-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 19224
HGNC: HGNC:9604
Homologene: 743
Tbx5
Name: T-box 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 21388
Homologene: 160
Olfr727
Name: olfactory receptor 727
Synonyms: GA_x6K02T2PMLR-5817082-5818056, MOR246-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 258316
Homologene: 84571
Cd177
Name: CD177 antigen
Synonyms: Pdp3, 1190003K14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 68891
Homologene: 49628
Akr1b10
Name: aldo-keto reductase family 1, member B10 (aldose reductase)
Synonyms: 2310005E10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 67861
Homologene: 128412
Mknk2
Name: MAP kinase-interacting serine/threonine kinase 2
Synonyms: Mnk2, 2010016G11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 17347
HGNC: HGNC:7111
Homologene: 49674
Gm5724
Name: predicted gene 5724
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 435927
Ppp2r2b
Name: protein phosphatase 2, regulatory subunit B, beta
Synonyms: 6330404L05Rik, PP2A-PR55B, PR55-BETA, SCA12, 2900026H06Rik, E130009M08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 72930
HGNC: HGNC:9305
Homologene: 55833
Plat
Name: plasminogen activator, tissue
Synonyms: t-PA, tPA, D8Ertd2e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 18791
HGNC: HGNC:9051
Homologene: 717
Olfr103
Name: olfactory receptor 103
Synonyms: MOR250-3, GA_x6K02T2PSCP-1798423-1797482, MOR250-8_p
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 258830
Homologene: 115555
Ccdc137
Name: coiled-coil domain containing 137
Synonyms: 3110023B02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 67291
Homologene: 34992
Tmem140
Name: transmembrane protein 140
Synonyms: 1110007F12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 68487
Homologene: 10116
Tmem200b
Name: transmembrane protein 200B
Synonyms: EG623230
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 623230
VEGA: 4
Homologene: 79738
Gm6158
Name: predicted gene 6158
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 620499
VEGA: 14
Gm7255
Name: predicted gene 7255
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 638950
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 20,585,711 bp
  • C to T, chromosome 1 at 36,552,978 bp
  • A to T, chromosome 1 at 73,952,864 bp
  • GCCC to GC, chromosome 1 at 88,232,257 bp
  • A to C, chromosome 1 at 90,779,352 bp
  • A to G, chromosome 1 at 91,355,578 bp
  • A to G, chromosome 1 at 171,936,533 bp
  • A to C, chromosome 1 at 188,995,693 bp
  • A to T, chromosome 2 at 31,905,667 bp
  • A to G, chromosome 2 at 36,237,282 bp
  • A to C, chromosome 2 at 52,280,501 bp
  • T to C, chromosome 2 at 118,121,237 bp
  • A to G, chromosome 2 at 131,173,281 bp
  • T to A, chromosome 3 at 98,328,339 bp
  • A to T, chromosome 4 at 117,927,676 bp
  • A to G, chromosome 4 at 131,922,537 bp
  • C to T, chromosome 5 at 66,969,235 bp
  • T to C, chromosome 5 at 73,075,893 bp
  • T to A, chromosome 5 at 119,836,922 bp
  • A to C, chromosome 5 at 137,898,899 bp
  • G to T, chromosome 6 at 34,392,106 bp
  • G to A, chromosome 6 at 34,872,962 bp
  • A to T, chromosome 6 at 38,074,183 bp
  • C to T, chromosome 6 at 42,269,272 bp
  • C to T, chromosome 6 at 58,855,760 bp
  • A to G, chromosome 6 at 110,646,136 bp
  • T to A, chromosome 6 at 113,304,488 bp
  • T to C, chromosome 6 at 130,179,109 bp
  • A to C, chromosome 6 at 141,767,454 bp
  • C to T, chromosome 7 at 19,738,273 bp
  • T to A, chromosome 7 at 19,760,101 bp
  • A to T, chromosome 7 at 23,435,910 bp
  • C to A, chromosome 7 at 24,744,316 bp
  • C to T, chromosome 7 at 28,329,015 bp
  • T to C, chromosome 7 at 47,502,928 bp
  • G to A, chromosome 7 at 119,472,421 bp
  • A to C, chromosome 7 at 123,410,830 bp
  • G to A, chromosome 7 at 126,374,329 bp
  • G to A, chromosome 7 at 127,880,499 bp
  • A to G, chromosome 8 at 22,772,311 bp
  • T to C, chromosome 8 at 41,259,260 bp
  • A to T, chromosome 8 at 104,516,702 bp
  • G to T, chromosome 9 at 56,260,289 bp
  • T to C, chromosome 9 at 119,956,971 bp
  • T to A, chromosome 10 at 25,993,915 bp
  • A to G, chromosome 10 at 60,364,263 bp
  • G to A, chromosome 10 at 80,671,769 bp
  • C to A, chromosome 11 at 21,997,037 bp
  • A to G, chromosome 11 at 69,678,964 bp
  • A to T, chromosome 11 at 110,177,066 bp
  • A to C, chromosome 11 at 120,366,749 bp
  • A to G, chromosome 11 at 120,462,515 bp
  • A to G, chromosome 12 at 4,259,333 bp
  • A to T, chromosome 12 at 51,744,879 bp
  • T to C, chromosome 12 at 112,821,863 bp
  • T to C, chromosome 13 at 11,700,354 bp
  • A to G, chromosome 13 at 41,008,145 bp
  • A to T, chromosome 14 at 24,070,090 bp
  • T to A, chromosome 14 at 31,172,440 bp
  • T to A, chromosome 14 at 50,127,437 bp
  • A to C, chromosome 14 at 65,979,728 bp
  • T to C, chromosome 15 at 76,347,611 bp
  • T to C, chromosome 15 at 79,728,536 bp
  • A to C, chromosome 15 at 99,944,646 bp
  • G to T, chromosome 16 at 17,309,397 bp
  • A to T, chromosome 17 at 37,336,931 bp
  • A to G, chromosome 17 at 49,070,500 bp
  • A to T, chromosome 17 at 88,986,046 bp
  • T to A, chromosome 18 at 22,522,718 bp
  • C to A, chromosome 18 at 42,688,461 bp
  • T to C, chromosome 18 at 68,339,281 bp
  • A to C, chromosome 19 at 12,650,263 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5062 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042652-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.