Strain Name:
C57BL/6J-MtgxR5073Btlr/Mmmh
Stock Number:
042662-MU
Citation ID:
RRID:MMRRC_042662-MU
Other Names:
R5073 (G1), C57BL/6J-MtgxR5073Btlr
Major Collection:

Gene Information

Gene Symbol: Rptor [MGI:1921620] (Mus musculus (mouse))
Name: regulatory associated protein of MTOR, complex 1
Synonyms: Rap, 4932417H02Rik, raptor
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Rgs9 [MGI:1338824] (Mus musculus (mouse))
Name: regulator of G-protein signaling 9
Synonyms: RGS9-1, Rgs9-2
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Dysf [MGI:1349385] (Mus musculus (mouse))
Name: dysferlin
Synonyms: 2310004N10Rik
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Nudt1 [MGI:109280] (Mus musculus (mouse))
Name: nudix (nucleoside diphosphate linked moiety X)-type motif 1
Synonyms: 8-oxo-7,8-dihydro-2'-dGTPase, Mth1
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Fryl [MGI:1919563] (Mus musculus (mouse))
Name: FRY like transcription coactivator
Synonyms: 2510002A14Rik, 2310004H21Rik, 9030227G01Rik
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Pck1 [MGI:97501] (Mus musculus (mouse))
Name: phosphoenolpyruvate carboxykinase 1, cytosolic
Synonyms: PEPCK, Pck-1
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Nudt5 [MGI:1858232] (Mus musculus (mouse))
Name: nudix (nucleoside diphosphate linked moiety X)-type motif 5
Chromosome: 2
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 14,754,313 bp
  • C to A, chromosome 1 at 43,954,736 bp
  • T to C, chromosome 1 at 72,339,029 bp
  • T to C, chromosome 1 at 93,022,505 bp
  • T to A, chromosome 2 at 5,864,387 bp
  • A to G, chromosome 2 at 32,673,261 bp
  • A to T, chromosome 2 at 36,251,260 bp
  • A to T, chromosome 2 at 63,979,693 bp
  • A to T, chromosome 2 at 77,124,378 bp
  • A to T, chromosome 2 at 86,978,322 bp
  • A to G, chromosome 2 at 91,054,570 bp
  • A to G, chromosome 2 at 120,491,820 bp
  • A to G, chromosome 2 at 120,963,486 bp
  • A to G, chromosome 2 at 164,490,304 bp
  • A to T, chromosome 2 at 169,962,573 bp
  • A to G, chromosome 2 at 173,156,977 bp
  • G to T, chromosome 3 at 41,287,934 bp
  • A to T, chromosome 3 at 65,819,794 bp
  • A to T, chromosome 3 at 108,079,233 bp
  • A to G, chromosome 3 at 108,911,168 bp
  • A to G, chromosome 3 at 116,124,388 bp
  • A to T, chromosome 3 at 122,933,946 bp
  • A to G, chromosome 4 at 25,254,780 bp
  • A to G, chromosome 4 at 64,020,411 bp
  • A to G, chromosome 4 at 115,247,663 bp
  • A to G, chromosome 4 at 120,717,517 bp
  • A to G, chromosome 4 at 135,705,146 bp
  • C to A, chromosome 4 at 149,469,138 bp
  • C to G, chromosome 4 at 155,656,776 bp
  • G to A, chromosome 5 at 24,438,762 bp
  • G to A, chromosome 5 at 25,516,883 bp
  • G to A, chromosome 5 at 30,903,059 bp
  • G to A, chromosome 5 at 73,074,767 bp
  • A to T, chromosome 5 at 114,452,952 bp
  • T to A, chromosome 5 at 115,946,843 bp
  • T to A, chromosome 5 at 137,795,519 bp
  • A to G, chromosome 5 at 137,835,412 bp
  • G to T, chromosome 5 at 140,331,907 bp
  • C to A, chromosome 5 at 147,683,939 bp
  • C to T, chromosome 6 at 52,620,094 bp
  • A to T, chromosome 6 at 55,345,535 bp
  • A to T, chromosome 6 at 84,137,272 bp
  • T to C, chromosome 6 at 113,310,254 bp
  • T to C, chromosome 6 at 121,222,903 bp
  • T to A, chromosome 6 at 139,720,147 bp
  • G to A, chromosome 7 at 98,073,218 bp
  • A to T, chromosome 7 at 141,859,262 bp
  • A to C, chromosome 7 at 144,896,839 bp
  • A to G, chromosome 8 at 3,159,475 bp
  • A to G, chromosome 8 at 4,301,741 bp
  • T to A, chromosome 8 at 54,690,236 bp
  • T to A, chromosome 8 at 66,386,368 bp
  • A to G, chromosome 8 at 83,233,547 bp
  • C to A, chromosome 8 at 95,841,650 bp
  • A to G, chromosome 8 at 110,538,473 bp
  • A to T, chromosome 8 at 123,318,140 bp
  • T to C, chromosome 9 at 18,754,822 bp
  • T to G, chromosome 9 at 80,288,008 bp
  • G to T, chromosome 9 at 103,046,726 bp
  • G to A, chromosome 9 at 108,563,336 bp
  • G to A, chromosome 9 at 108,574,366 bp
  • A to G, chromosome 10 at 31,357,920 bp
  • G to T, chromosome 10 at 75,519,393 bp
  • C to T, chromosome 10 at 76,222,556 bp
  • C to A, chromosome 10 at 79,918,762 bp
  • A to G, chromosome 10 at 80,784,646 bp
  • C to T, chromosome 10 at 81,090,137 bp
  • A to T, chromosome 10 at 86,863,558 bp
  • CACTCGTCATACT to CACT, chromosome 10 at 89,430,958 bp
  • A to T, chromosome 10 at 93,514,042 bp
  • T to C, chromosome 10 at 127,707,419 bp
  • T to A, chromosome 11 at 36,068,381 bp
  • A to G, chromosome 11 at 49,095,534 bp
  • A to G, chromosome 11 at 82,037,158 bp
  • A to G, chromosome 11 at 85,371,132 bp
  • A to G, chromosome 11 at 95,750,064 bp
  • G to A, chromosome 11 at 96,251,782 bp
  • T to A, chromosome 11 at 99,243,970 bp
  • T to G, chromosome 11 at 101,261,188 bp
  • C to A, chromosome 11 at 109,227,331 bp
  • G to A, chromosome 11 at 114,767,227 bp
  • T to C, chromosome 11 at 119,896,479 bp
  • C to T, chromosome 11 at 120,763,186 bp
  • G to A, chromosome 12 at 8,005,219 bp
  • G to A, chromosome 13 at 102,738,883 bp
  • A to G, chromosome 14 at 31,162,962 bp
  • G to A, chromosome 14 at 51,920,455 bp
  • T to A, chromosome 14 at 52,463,575 bp
  • A to G, chromosome 14 at 57,566,007 bp
  • G to A, chromosome 15 at 34,123,762 bp
  • A to T, chromosome 15 at 37,397,234 bp
  • A to T, chromosome 15 at 64,787,358 bp
  • A to C, chromosome 15 at 66,735,252 bp
  • A to G, chromosome 15 at 81,022,426 bp
  • C to T, chromosome 15 at 82,403,753 bp
  • A to G, chromosome 16 at 3,746,840 bp
  • A to T, chromosome 16 at 4,696,425 bp
  • A to T, chromosome 16 at 5,088,878 bp
  • C to A, chromosome 16 at 32,754,529 bp
  • A to T, chromosome 16 at 35,834,707 bp
  • A to G, chromosome 16 at 87,427,740 bp
  • C to T, chromosome 17 at 24,374,300 bp
  • T to C, chromosome 17 at 78,281,347 bp
  • C to A, chromosome 17 at 83,463,577 bp
  • C to A, chromosome 18 at 34,651,689 bp
  • A to G, chromosome 18 at 37,715,972 bp
  • T to A, chromosome 19 at 9,003,231 bp
  • A to G, chromosome 19 at 37,210,808 bp
  • A to G, chromosome X at 7,944,797 bp
  • A to G, chromosome X at 106,109,768 bp
  • C to T, chromosome X at 112,349,843 bp
  • T to C, chromosome X at 120,312,846 bp
  • C to T, chromosome X at 135,842,597 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5073 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042662-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.