Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR5120Btlr/Mmmh
Stock Number:
042708-MU
Citation ID:
RRID:MMRRC_042708-MU
Other Names:
R5120 (G1), C57BL/6J-MtgxR5120Btlr
Major Collection:

Strain Information

Ep400
Name: E1A binding protein p400
Synonyms: p400, mDomino, 1700020J09Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 75560
Homologene: 38779
Plekha7
Name: pleckstrin homology domain containing, family A member 7
Synonyms: A430081P20Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233765
Homologene: 52172
Ctnna1
Name: catenin alpha 1
Synonyms: alpha(E)-catenin, alpha E catenin, Catna1, 2010010M04Rik, catenin (cadherin associated protein), alpha 1
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 12385
VEGA: 18
HGNC: HGNC:2509
Homologene: 1433
Camsap3
Name: calmodulin regulated spectrin-associated protein family, member 3
Synonyms: Nezha, 2310057J16Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 69697
Homologene: 18966
Cwf19l2
Name: CWF19 like cell cycle control factor 2
Synonyms: 3230401L03Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 244672
Homologene: 12366
Trim6
Name: tripartite motif-containing 6
Synonyms: D7Ertd684e, C430046K18Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 94088
Homologene: 14381
Kif16b
Name: kinesin family member 16B
Synonyms: N-3 kinesin, 8430434E15Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16558
Homologene: 135708
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 40,854,901 bp
  • T to C, chromosome 1 at 63,623,043 bp
  • T to A, chromosome 1 at 135,973,502 bp
  • T to C, chromosome 1 at 139,753,103 bp
  • T to C, chromosome 2 at 24,405,438 bp
  • T to C, chromosome 2 at 51,932,423 bp
  • T to C, chromosome 2 at 111,409,240 bp
  • A to T, chromosome 2 at 112,130,558 bp
  • A to G, chromosome 2 at 131,179,818 bp
  • G to A, chromosome 2 at 136,083,229 bp
  • A to C, chromosome 2 at 142,848,339 bp
  • A to T, chromosome 2 at 146,143,160 bp
  • G to T, chromosome 2 at 146,412,084 bp
  • G to A, chromosome 2 at 181,797,620 bp
  • A to T, chromosome 3 at 93,148,896 bp
  • T to A, chromosome 3 at 96,578,479 bp
  • G to C, chromosome 3 at 108,393,120 bp
  • T to C, chromosome 3 at 124,292,085 bp
  • T to C, chromosome 3 at 154,311,695 bp
  • T to C, chromosome 3 at 155,129,384 bp
  • T to C, chromosome 4 at 48,459,272 bp
  • C to A, chromosome 4 at 83,558,526 bp
  • A to G, chromosome 4 at 99,897,491 bp
  • G to T, chromosome 4 at 121,039,772 bp
  • C to A, chromosome 4 at 126,035,827 bp
  • T to C, chromosome 4 at 127,051,437 bp
  • A to G, chromosome 5 at 21,269,936 bp
  • T to C, chromosome 5 at 52,582,882 bp
  • T to C, chromosome 5 at 52,660,661 bp
  • A to G, chromosome 5 at 90,264,002 bp
  • T to A, chromosome 5 at 101,868,106 bp
  • G to T, chromosome 5 at 110,756,358 bp
  • C to G, chromosome 5 at 113,957,398 bp
  • T to C, chromosome 5 at 136,064,191 bp
  • A to T, chromosome 6 at 72,632,974 bp
  • A to G, chromosome 6 at 97,134,315 bp
  • T to A, chromosome 6 at 131,678,816 bp
  • T to A, chromosome 6 at 142,656,618 bp
  • A to G, chromosome 7 at 25,010,640 bp
  • C to T, chromosome 7 at 43,999,022 bp
  • A to T, chromosome 7 at 44,256,971 bp
  • C to T, chromosome 7 at 44,862,403 bp
  • C to T, chromosome 7 at 101,977,437 bp
  • T to A, chromosome 7 at 104,228,240 bp
  • C to T, chromosome 7 at 116,140,719 bp
  • T to C, chromosome 7 at 122,071,750 bp
  • T to C, chromosome 7 at 140,099,652 bp
  • G to A, chromosome 8 at 3,600,680 bp
  • G to T, chromosome 8 at 23,180,202 bp
  • T to A, chromosome 8 at 25,934,488 bp
  • T to C, chromosome 8 at 37,526,266 bp
  • T to C, chromosome 8 at 61,945,906 bp
  • C to A, chromosome 8 at 70,048,667 bp
  • A to T, chromosome 8 at 72,859,341 bp
  • T to C, chromosome 8 at 83,566,977 bp
  • T to A, chromosome 8 at 116,515,698 bp
  • C to T, chromosome 9 at 3,418,761 bp
  • T to C, chromosome 9 at 30,921,576 bp
  • T to A, chromosome 9 at 39,702,070 bp
  • T to G, chromosome 9 at 46,242,737 bp
  • T to C, chromosome 9 at 105,669,009 bp
  • T to A, chromosome 10 at 128,314,995 bp
  • A to G, chromosome 11 at 59,210,235 bp
  • A to T, chromosome 11 at 62,780,725 bp
  • A to C, chromosome 11 at 73,786,964 bp
  • A to G, chromosome 11 at 82,960,928 bp
  • A to G, chromosome 11 at 101,325,714 bp
  • A to T, chromosome 11 at 107,073,385 bp
  • A to G, chromosome 11 at 120,811,391 bp
  • T to C, chromosome 12 at 21,343,019 bp
  • A to G, chromosome 12 at 75,632,638 bp
  • C to A, chromosome 12 at 85,139,348 bp
  • G to T, chromosome 12 at 115,145,786 bp
  • T to A, chromosome 13 at 6,662,656 bp
  • C to A, chromosome 13 at 49,901,948 bp
  • T to A, chromosome 13 at 81,669,192 bp
  • C to T, chromosome 14 at 20,650,654 bp
  • A to T, chromosome 15 at 6,827,275 bp
  • G to A, chromosome 15 at 48,673,495 bp
  • C to A, chromosome 15 at 89,094,305 bp
  • T to C, chromosome 15 at 96,419,542 bp
  • T to C, chromosome 15 at 98,898,157 bp
  • C to A, chromosome 16 at 4,044,088 bp
  • T to C, chromosome 16 at 30,565,502 bp
  • A to G, chromosome 16 at 88,751,570 bp
  • T to A, chromosome 17 at 24,518,744 bp
  • T to C, chromosome 17 at 36,325,156 bp
  • A to T, chromosome 17 at 38,067,266 bp
  • A to T, chromosome 17 at 80,408,248 bp
  • G to A, chromosome 18 at 35,182,554 bp
  • G to A, chromosome 18 at 35,979,810 bp
  • A to T, chromosome 19 at 7,304,274 bp
  • C to T, chromosome 19 at 12,461,429 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5120 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042708-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.