Strain Name:
C57BL/6J-MtgxR5123Btlr/Mmmh
Stock Number:
042711-MU
Citation ID:
RRID:MMRRC_042711-MU
Other Names:
R5123 (G1), C57BL/6J-MtgxR5123Btlr
Major Collection:

Gene Information

Myo10
Name: myosin X
Synonyms: myosin-X, D15Ertd600e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 17909
HGNC: HGNC:7593
Homologene: 36328
Epha2
Name: Eph receptor A2
Synonyms: Sek-2, Eck, Sek2, Myk2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 13836
HGNC: HGNC:3386
Homologene: 20929
Col3a1
Name: collagen, type III, alpha 1
Synonyms: Col3a-1, Tsk-2, Tsk2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12825
HGNC: HGNC:2201
Homologene: 55433
Trim59
Name: tripartite motif-containing 59
Synonyms: 2700022F13Rik, TSBF1, Mrf1, 2310035M22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 66949
Homologene: 32644
Gnaq
Name: guanine nucleotide binding protein, alpha q polypeptide
Synonyms: G alpha q, Gq, Galphaq, 6230401I02Rik, 1110005L02Rik, Dsk10, Dsk1, GqI
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 14682
HGNC: HGNC:4390
Homologene: 1566
Adsl
Name: adenylosuccinate lyase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 11564
HGNC: HGNC:291
Homologene: 12
Net1
Name: neuroepithelial cell transforming gene 1
Synonyms: mNET1, Net1 homolog, 9530071N24Rik, 0610025H04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 56349
VEGA: 13
Homologene: 4283
Setd2
Name: SET domain containing 2
Synonyms: 4921524K10Rik, KMT3A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235626
Homologene: 56493
Cdc27
Name: cell division cycle 27
Synonyms: APC3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217232
HGNC: HGNC:1728
Homologene: 960
Traf3
Name: TNF receptor-associated factor 3
Synonyms: LAP1, CAP-1, CD40bp, CRAF1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 22031
Homologene: 7981
Sgo2a
Name: shugoshin 2A
Synonyms: 5730576N04Rik, Tripin, 1110007N04Rik, D1Ertd8e, Sgol2, Sgol2a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 68549
Homologene: 51867
Apob
Name: apolipoprotein B
Synonyms: apob-100, apob-48
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 238055
HGNC: HGNC:603
Homologene: 328
Zfp74
Name: zinc finger protein 74
Synonyms: Zfp66, KRAB8, 2810054M15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 72723
Homologene: 51861
Hjurp
Name: Holliday junction recognition protein
Synonyms: C330011F01Rik, A730008H23Rik, 6430706D22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 381280
Homologene: 10184
Gcnt2
Name: glucosaminyl (N-acetyl) transferase 2, I-branching enzyme
Synonyms: IGnTC, IGnTB, IGnTA, 5330430K10Rik, IGnT
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 14538
VEGA: 13
HGNC: HGNC:4204
Homologene: 41535
Eif2b3
Name: eukaryotic translation initiation factor 2B, subunit 3
Synonyms: 1190002P15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 108067
HGNC: HGNC:3259
Homologene: 7005
Stk11
Name: serine/threonine kinase 11
Synonyms: Lkb1, Par-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 20869
Homologene: 393
Eml5
Name: echinoderm microtubule associated protein like 5
Synonyms: C130068M19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 319670
VEGA: 12
Homologene: 26807
Zfp280b
Name: zinc finger protein 280B
Synonyms: D10Jhu82e, Suhw2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 64453
VEGA: 10
Homologene: 62794
Abca3
Name: ATP-binding cassette, sub-family A (ABC1), member 3
Synonyms: ABC-C, Abc3, 1810036E22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 27410
HGNC: HGNC:33
Homologene: 37437
Trappc11
Name: trafficking protein particle complex 11
Synonyms: D030016E14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 320714
Homologene: 11076
Olfr1301
Name: olfactory receptor 1301
Synonyms: GA_x6K02T2Q125-72805651-72806589, MOR248-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258889
Homologene: 74057
Dscam
Name: DS cell adhesion molecule
Synonyms: 4932410A21Rik, Down syndrome cell adhesion molecule
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 13508
HGNC: HGNC:3039
Homologene: 74393
Clca3a2
Name: chloride channel accessory 3A2
Synonyms: Clca2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 80797
Homologene: 77224
Spata6
Name: spermatogenesis associated 6
Synonyms: Hash, 1700062C23Rik, KRP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 67946
Homologene: 10410
Haus5
Name: HAUS augmin-like complex, subunit 5
Synonyms: 2310022K01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 71909
Homologene: 18969
Vmn2r111
Name: vomeronasal 2, receptor 111
Synonyms: EG210876
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 210876
Homologene: 86604
Ttc21a
Name: tetratricopeptide repeat domain 21A
Synonyms: 4921538N17Rik, Thm2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 74052
Homologene: 14728
Zfp607a
Name: zinc finger protein 607A
Synonyms: 4732475C15Rik, Zfp607
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 545938
Homologene: 134321
Wdr66
Name: WD repeat domain 66
Synonyms: 4930415N18Rik, 4933428F06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 269701
Homologene: 16964
Prss1
Name: protease, serine 1 (trypsin 1)
Synonyms: PRSS1, Try-1, Try1, Trygn16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 114228
Homologene: 88408
Usp13
Name: ubiquitin specific peptidase 13 (isopeptidase T-3)
Synonyms: IsoT-3, ISOT3, 2700071E21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 72607
Homologene: 68372
Tkt
Name: transketolase
Synonyms: p68
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 21881
Homologene: 68154
Cacnb3
Name: calcium channel, voltage-dependent, beta 3 subunit
Synonyms: Cchb3, Beta3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 12297
VEGA: 15
HGNC: HGNC:1403
Homologene: 20187
Vmn2r17
Name: vomeronasal 2, receptor 17
Synonyms: EG384221
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 384221
Homologene: 104825
D7Ertd443e
Name: DNA segment, Chr 7, ERATO Doi 443, expressed
Synonyms: 4933400E14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 71007
Homologene: 18998
Pramel11
Name: PRAME like 11
Synonyms: Gm13099, Pramef6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 195555
Homologene: 133722
Adh6b
Name: alcohol dehydrogenase 6B (class V)
Synonyms: Adh5b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 100417514
Homologene: 139477
Pcsk4
Name: proprotein convertase subtilisin/kexin type 4
Synonyms: PC4, SPC5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 18551
HGNC: HGNC:8746
Homologene: 22495
Atp6ap1l
Name: ATPase, H+ transporting, lysosomal accessory protein 1-like
Synonyms: EG435376
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 435376
Homologene: 28130
Igsf5
Name: immunoglobulin superfamily, member 5
Synonyms: 2010003D20Rik, Igsf5, Jam4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 72058
HGNC: HGNC:5952
Homologene: 12437
Rnf133
Name: ring finger protein 133
Synonyms: Greul2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 386611
Homologene: 44549
Slc2a5
Name: solute carrier family 2 (facilitated glucose transporter), member 5
Synonyms: GLUT5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 56485
Homologene: 74459
Filip1l
Name: filamin A interacting protein 1-like
Synonyms: 4631422O05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 78749
Homologene: 37121
Vmn1r238
Name: vomeronasal 1 receptor, 238
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 100312476
Homologene: 128342
Gm3150
Name: predicted gene 3150
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 100041120
Olfr1273-ps
Name: olfactory receptor 1273, pseudogene
Synonyms: GA_x6K02T2Q125-51650793-51649870, MOR227-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258977
Homologene: 133649
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 45,333,596 bp
  • A to T, chromosome 1 at 58,016,567 bp
  • A to T, chromosome 1 at 88,275,050 bp
  • C to A, chromosome 2 at 90,296,168 bp
  • T to A, chromosome 2 at 111,754,552 bp
  • A to T, chromosome 3 at 32,915,798 bp
  • T to C, chromosome 3 at 69,037,734 bp
  • A to G, chromosome 3 at 138,357,689 bp
  • T to A, chromosome 3 at 144,806,343 bp
  • T to G, chromosome 4 at 111,768,795 bp
  • T to A, chromosome 4 at 117,022,211 bp
  • T to A, chromosome 4 at 141,308,865 bp
  • A to T, chromosome 4 at 143,897,136 bp
  • C to A, chromosome 4 at 150,139,805 bp
  • A to G, chromosome 5 at 109,427,908 bp
  • A to G, chromosome 5 at 123,273,633 bp
  • A to T, chromosome 6 at 23,649,260 bp
  • A to G, chromosome 6 at 41,463,197 bp
  • A to G, chromosome 7 at 27,879,098 bp
  • A to T, chromosome 7 at 29,934,733 bp
  • A to T, chromosome 7 at 30,654,226 bp
  • A to G, chromosome 7 at 134,349,668 bp
  • T to C, chromosome 8 at 47,513,402 bp
  • G to T, chromosome 9 at 110,617,527 bp
  • A to G, chromosome 9 at 119,952,212 bp
  • A to G, chromosome 10 at 76,039,349 bp
  • G to A, chromosome 10 at 80,127,941 bp
  • C to A, chromosome 10 at 80,322,145 bp
  • T to C, chromosome 11 at 104,508,398 bp
  • T to A, chromosome 12 at 8,007,630 bp
  • T to C, chromosome 12 at 98,874,512 bp
  • G to A, chromosome 12 at 111,243,518 bp
  • C to T, chromosome 13 at 3,886,623 bp
  • T to A, chromosome 13 at 40,918,355 bp
  • T to A, chromosome 13 at 90,898,898 bp
  • T to C, chromosome 14 at 30,565,646 bp
  • A to G, chromosome 15 at 25,726,483 bp
  • T to C, chromosome 15 at 72,913,366 bp
  • T to A, chromosome 15 at 80,952,294 bp
  • A to G, chromosome 15 at 98,639,869 bp
  • A to C, chromosome 16 at 57,570,662 bp
  • A to G, chromosome 16 at 96,373,079 bp
  • T to C, chromosome 16 at 96,772,437 bp
  • T to A, chromosome 17 at 22,571,143 bp
  • G to A, chromosome 17 at 24,384,460 bp
  • T to C, chromosome 18 at 3,123,243 bp
  • A to G, chromosome 19 at 16,332,085 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5123 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042711-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.