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Strain Name:
C57BL/6J-MtgxR5162Btlr/Mmmh
Stock Number:
042744-MU
Citation ID:
RRID:MMRRC_042744-MU
Other Names:
R5162 (G1), C57BL/6J-MtgxR5162Btlr
Major Collection:

Strain Information

Faah
Name: fatty acid amide hydrolase
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 14073
HGNC: HGNC:3553
Homologene: 68184
Gcm2
Name: glial cells missing homolog 2
Synonyms: Gcm1-rs2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 107889
VEGA: 13
HGNC: HGNC:4198
Homologene: 3490
Ehmt1
Name: euchromatic histone methyltransferase 1
Synonyms: 9230102N17Rik, KMT1D
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 77683
Homologene: 11698
Spred1
Name: sprouty protein with EVH-1 domain 1, related sequence
Synonyms: Spred-1, 5730461F13Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 114715
Homologene: 24919
Enpp2
Name: ectonucleotide pyrophosphatase/phosphodiesterase 2
Synonyms: Autotaxin, Npps2, PD-Ialpha, Pdnp2, ATX
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 18606
HGNC: HGNC:3357
Homologene: 4526
Tyw5
Name: tRNA-yW synthesizing protein 5
Synonyms: 1110034B05Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 68736
Homologene: 72085
Msh2
Name: mutS homolog 2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 17685
HGNC: HGNC:7325
Homologene: 210
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 13,175,172 bp
  • T to C, chromosome 1 at 57,401,459 bp
  • G to T, chromosome 1 at 193,113,781 bp
  • G to T, chromosome 2 at 24,877,497 bp
  • T to A, chromosome 2 at 25,343,353 bp
  • C to A, chromosome 2 at 77,015,137 bp
  • T to C, chromosome 2 at 117,177,621 bp
  • A to G, chromosome 2 at 119,292,942 bp
  • T to A, chromosome 2 at 139,631,343 bp
  • A to T, chromosome 3 at 62,493,780 bp
  • T to C, chromosome 3 at 79,481,777 bp
  • T to C, chromosome 3 at 88,747,842 bp
  • T to C, chromosome 3 at 108,940,050 bp
  • T to C, chromosome 4 at 101,102,724 bp
  • A to G, chromosome 4 at 106,654,538 bp
  • A to T, chromosome 4 at 116,000,741 bp
  • A to T, chromosome 4 at 144,394,912 bp
  • A to G, chromosome 5 at 27,399,015 bp
  • T to C, chromosome 5 at 135,783,475 bp
  • T to C, chromosome 5 at 136,991,307 bp
  • G to A, chromosome 5 at 142,721,452 bp
  • T to A, chromosome 5 at 145,369,083 bp
  • G to T, chromosome 6 at 41,771,772 bp
  • T to A, chromosome 6 at 50,178,515 bp
  • A to G, chromosome 6 at 57,360,843 bp
  • A to G, chromosome 6 at 92,074,234 bp
  • T to A, chromosome 6 at 137,443,594 bp
  • C to G, chromosome 7 at 24,276,332 bp
  • C to T, chromosome 7 at 27,281,361 bp
  • T to C, chromosome 7 at 46,892,426 bp
  • T to C, chromosome 7 at 97,059,820 bp
  • T to C, chromosome 8 at 27,227,320 bp
  • G to A, chromosome 8 at 45,025,809 bp
  • A to T, chromosome 8 at 56,569,319 bp
  • T to C, chromosome 8 at 106,133,298 bp
  • A to T, chromosome 9 at 31,340,418 bp
  • A to G, chromosome 9 at 104,197,209 bp
  • T to A, chromosome 9 at 124,293,671 bp
  • T to A, chromosome 9 at 124,350,394 bp
  • T to A, chromosome 10 at 41,357,657 bp
  • T to A, chromosome 10 at 85,133,286 bp
  • G to C, chromosome 11 at 50,052,895 bp
  • C to A, chromosome 11 at 102,430,554 bp
  • T to C, chromosome 11 at 121,429,584 bp
  • T to A, chromosome 12 at 73,329,985 bp
  • T to C, chromosome 13 at 41,103,655 bp
  • T to A, chromosome 13 at 100,223,406 bp
  • C to T, chromosome 13 at 100,300,600 bp
  • C to T, chromosome 13 at 104,181,174 bp
  • C to A, chromosome 14 at 51,456,163 bp
  • T to C, chromosome 15 at 54,847,296 bp
  • T to C, chromosome 16 at 16,260,336 bp
  • A to T, chromosome 16 at 36,312,556 bp
  • T to A, chromosome 17 at 36,118,951 bp
  • T to A, chromosome 17 at 37,952,364 bp
  • C to A, chromosome 17 at 87,723,413 bp
  • T to C, chromosome 18 at 24,654,052 bp
  • T to C, chromosome 18 at 64,561,662 bp
  • C to T, chromosome X at 12,040,486 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5162 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042744-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.