Strain Name:
C57BL/6J-MtgxR5204Btlr/Mmmh
Stock Number:
042779-MU
Citation ID:
RRID:MMRRC_042779-MU
Other Names:
R5204 (G1), C57BL/6J-MtgxR5204Btlr
Major Collection:

Gene Information

Tln2
Name: talin 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 70549
VEGA: 9
Homologene: 56692
Cdh3
Name: cadherin 3
Synonyms: P-cadherin, Cadp, Pcad
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 12560
HGNC: HGNC:1762
Homologene: 20425
Otx1
Name: orthodenticle homeobox 1
Synonyms: A730044F23Rik, jv
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18423
HGNC: HGNC:8521
Homologene: 7875
Hdac5
Name: histone deacetylase 5
Synonyms: mHDA1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 15184
Homologene: 3995
Aopep
Name: aminopeptidase O
Synonyms: 2010111I01Rik, ApO
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 72061
HGNC: HGNC:1361
Homologene: 66273
Usp15
Name: ubiquitin specific peptidase 15
Synonyms: Gcap18, 4921514G19Rik, E430033I05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 14479
VEGA: 10
Homologene: 101542
Rufy3
Name: RUN and FYVE domain containing 3
Synonyms: Rpipx, D5Bwg0860e, 2810428M05Rik, 6330416M07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 52822
Homologene: 87809
Pi4ka
Name: phosphatidylinositol 4-kinase alpha
Synonyms: Pik4ca
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 224020
HGNC: HGNC:8983
Homologene: 11171
Arid1b
Name: AT rich interactive domain 1B (SWI-like)
Synonyms: 9330189K18Rik, B230217J03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 239985
Homologene: 32344
Zfp866
Name: zinc finger protein 866
Synonyms: D330038O06Rik, 9830167H18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 330788
Clock
Name: circadian locomotor output cycles kaput
Synonyms: bHLHe8, 5330400M04Rik, KAT13D
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 12753
HGNC: HGNC:2082
Homologene: 3603
Pcdh20
Name: protocadherin 20
Synonyms: PCDH13, C630015B17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 219257
Homologene: 11277
Gpx7
Name: glutathione peroxidase 7
Synonyms: 3110050F08Rik, GPX6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 67305
HGNC: HGNC:4559
Homologene: 128491
Mcm6
Name: minichromosome maintenance complex component 6
Synonyms: D1Wsu22e, Mcmd6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 17219
HGNC: HGNC:6949
Homologene: 4322
Abt1
Name: activator of basal transcription 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 30946
Homologene: 134288
Rufy1
Name: RUN and FYVE domain containing 1
Synonyms: ZFYVE12, 3000002E04Rik, Rabip4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216724
Homologene: 23522
Clec4b1
Name: C-type lectin domain family 4, member b1
Synonyms: mDcar2, 1810046I24Rik, 1810046I24Rik, DCAR, DCARbeta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 69810
Homologene: 130350
Ttn
Name: titin
Synonyms: mdm, 2310036G12Rik, D830007G01Rik, shru, L56, 2310074I15Rik, 2310057K23Rik, connectin, D330041I19Rik, 1100001C23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Trpm3
Name: transient receptor potential cation channel, subfamily M, member 3
Synonyms: B930001P07Rik, MLSN2, 6330504P12Rik, LTRPC3, melastatin 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 226025
VEGA: 19
Homologene: 62287
Pkhd1l1
Name: polycystic kidney and hepatic disease 1-like 1
Synonyms: PKHDL1, fibrocystin L, D86 mRNA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 192190
Homologene: 16332
Chrd
Name: chordin
Synonyms: Chd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 12667
HGNC: HGNC:1949
Homologene: 2774
Hrc
Name: histidine rich calcium binding protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 15464
HGNC: HGNC:5178
Homologene: 137234
Klhl5
Name: kelch-like 5
Synonyms: 1300013C10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 71778
HGNC: HGNC:6356
Homologene: 56736
Slco1b2
Name: solute carrier organic anion transporter family, member 1b2
Synonyms: Slc21a10, Slc21a6, mlst-1, Oatp1b2, 7330442B20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 28253
Homologene: 75119
Cav1
Name: caveolin 1, caveolae protein
Synonyms: Cav-1, caveolin-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12389
HGNC: HGNC:1527
Homologene: 1330
Nrxn1
Name: neurexin I
Synonyms: alpha-latrotoxin receptor (calcium-dependent), neurexin I beta, neurexin I beta, neurexin I alpha, neurexin I alpha, neurexin I beta, neurexin I alpha, 1700062G21Rik, 9330127H16Rik, A230068P09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 18189
HGNC: HGNC:8008
Homologene: 21005
Sema5a
Name: sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A
Synonyms: semF, Semaf, M-Sema D, 9130201M22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 20356
VEGA: 15
Homologene: 2949
Pcdhb12
Name: protocadherin beta 12
Synonyms: PcdhbL, Pcdh3, Pcdhb5F
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93883
Homologene: 87126
Hivep3
Name: human immunodeficiency virus type I enhancer binding protein 3
Synonyms: Krc, Schnurri-3, 2900056N03Rik, E030045D18Rik, Shn3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 16656
Homologene: 7803
Cd7
Name: CD7 antigen
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 12516
HGNC: HGNC:1695
Homologene: 4471
Bivm
Name: basic, immunoglobulin-like variable motif containing
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 246229
Homologene: 9783
Tmem30c
Name: transmembrane protein 30C
Synonyms: 4933401B01Rik, 4933409A18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 71027
Homologene: 78138
Olfr609
Name: olfactory receptor 609
Synonyms: MOR9-1, GA_x6K02T2PBJ9-6207735-6206776
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 259086
Homologene: 64952
Slc4a1ap
Name: solute carrier family 4 (anion exchanger), member 1, adaptor protein
Synonyms: kanadaptin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 20534
Homologene: 7543
Tor3a
Name: torsin family 3, member A
Synonyms: Adir
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 30935
Homologene: 23359
Igkv4-80
Name: immunoglobulin kappa variable 4-80
Synonyms: Gm16729
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 545848
Gm26850
Name: predicted gene, 26850
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Gm15419
Name: predicted gene 15419
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Gm27029
Name: predicted gene, 27029
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to C, chromosome 1 at 44,138,578 bp
  • A to G, chromosome 1 at 128,333,638 bp
  • A to G, chromosome 1 at 156,655,700 bp
  • T to A, chromosome 2 at 76,730,212 bp
  • A to G, chromosome 3 at 63,963,746 bp
  • T to C, chromosome 4 at 108,403,315 bp
  • T to A, chromosome 4 at 120,103,856 bp
  • T to C, chromosome 5 at 31,527,363 bp
  • G to T, chromosome 5 at 65,131,438 bp
  • A to T, chromosome 5 at 76,243,170 bp
  • C to T, chromosome 5 at 88,648,309 bp
  • T to C, chromosome 6 at 17,339,255 bp
  • A to C, chromosome 6 at 69,016,665 bp
  • T to A, chromosome 6 at 123,071,535 bp
  • A to T, chromosome 6 at 141,652,415 bp
  • A to G, chromosome 7 at 45,335,704 bp
  • C to T, chromosome 7 at 103,492,540 bp
  • T to A, chromosome 8 at 11,398,651 bp
  • A to G, chromosome 8 at 69,766,040 bp
  • T to C, chromosome 8 at 106,544,239 bp
  • T to A, chromosome 9 at 67,354,482 bp
  • A to T, chromosome 10 at 123,113,640 bp
  • C to A, chromosome 11 at 21,997,037 bp
  • C to T, chromosome 11 at 50,406,434 bp
  • A to T, chromosome 11 at 101,406,926 bp
  • G to A, chromosome 11 at 102,195,804 bp
  • A to T, chromosome 11 at 121,038,034 bp
  • G to A, chromosome 13 at 23,422,668 bp
  • G to A, chromosome 13 at 63,033,090 bp
  • G to T, chromosome 14 at 88,468,915 bp
  • G to T, chromosome 15 at 32,686,647 bp
  • A to C, chromosome 15 at 44,547,041 bp
  • A to T, chromosome 16 at 17,359,045 bp
  • A to T, chromosome 16 at 20,736,072 bp
  • T to C, chromosome 16 at 57,270,022 bp
  • T to C, chromosome 17 at 5,343,041 bp
  • A to T, chromosome 17 at 90,162,364 bp
  • T to A, chromosome 18 at 37,436,089 bp
  • T to A, chromosome 19 at 22,448,341 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5204 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042779-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.