Strain Name:
C57BL/6J-MtgxR5224Btlr/Mmmh
Stock Number:
042797-MU
Citation ID:
RRID:MMRRC_042797-MU
Other Names:
R5224 (G1), C57BL/6J-MtgxR5224Btlr
Major Collection:

Gene Information

Mga
Name: MAX gene associated
Synonyms: Mga, Mad5, C130042M01Rik, D030062C11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 29808
Homologene: 49351
Osbpl1a
Name: oxysterol binding protein-like 1A
Synonyms: LOC328902, G430090F17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 64291
Homologene: 84746
Btbd11
Name: BTB (POZ) domain containing 11
Synonyms: 6330404E16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 74007
Homologene: 72536
Ldlrad3
Name: low density lipoprotein receptor class A domain containing 3
Synonyms: Lrad3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 241576
Homologene: 18377
Camk1g
Name: calcium/calmodulin-dependent protein kinase I gamma
Synonyms: CLICK-III, CaMKIgamma
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 215303
Homologene: 48706
Washc2
Name: WASH complex subunit 2`
Synonyms: C530005J20Rik, D6Wsu116e, Fam21
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 28006
Homologene: 41686
Erp44
Name: endoplasmic reticulum protein 44
Synonyms: 1110001E24Rik, Txndc4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 76299
Homologene: 12638
Suco
Name: SUN domain containing ossification factor
Synonyms: osteopotentia, Opt, AI848100
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226551
HGNC: HGNC:1240
Homologene: 32212
Gphn
Name: gephyrin
Synonyms: geph, 5730552E08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 268566
VEGA: 12
Homologene: 10820
Hnrnpul1
Name: heterogeneous nuclear ribonucleoprotein U-like 1
Synonyms: E1B-AP5, E1BAP5, E130317O14Rik, Hnrnpul, Hnrpul1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 232989
Homologene: 31419
Ppp1r12a
Name: protein phosphatase 1, regulatory subunit 12A
Synonyms: 1200015F06Rik, Mypt1, D10Ertd625e, 5730577I22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 17931
VEGA: 10
HGNC: HGNC:7618
Homologene: 1855
Nav2
Name: neuron navigator 2
Synonyms: POMFIL2, Unc53H2, HELAD1, RAINB2, 5330421F07Rik, Rainb1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 78286
Homologene: 52330
Depdc1b
Name: DEP domain containing 1B
Synonyms: XTP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218581
Homologene: 10157
Cct3
Name: chaperonin containing Tcp1, subunit 3 (gamma)
Synonyms: TriC-P5, Cctg, Tcp1-rs3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 12462
HGNC: HGNC:1616
Homologene: 4373
Tram1
Name: translocating chain-associating membrane protein 1
Synonyms: TRAMP, 1810049E02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 72265
Homologene: 8621
Ahi1
Name: Abelson helper integration site 1
Synonyms: Ahi-1, 1700015F03Rik, D10Bwg0629e, Jouberin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 52906
VEGA: 10
Homologene: 9762
Plpp1
Name: phospholipid phosphatase 1
Synonyms: mPAP, Lipid phosphate phosphatase 1, LPP1, LPP-1, Hpic53, Ppap2a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 19012
VEGA: 13
HGNC: HGNC:9228
Homologene: 134049
Ganab
Name: alpha glucosidase 2 alpha neutral subunit
Synonyms: GluII, G2an
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 14376
VEGA: 19
HGNC: HGNC:4138
Homologene: 5426
Lypd8
Name: LY6/PLAUR domain containing 8
Synonyms: 2210415F13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 70163
Homologene: 52815
Jaml
Name: junction adhesion molecule like
Synonyms: LOC270152, Amica1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 270152
Homologene: 17708
Muc19
Name: mucin 19
Synonyms: apomucin, sld
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239611
Muc5ac
Name: mucin 5, subtypes A and C, tracheobronchial/gastric
Synonyms: MGM, 2210005L13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 17833
HGNC: HGNC:7515
Homologene: 137237
Zfp354a
Name: zinc finger protein 354A
Synonyms: kid1, Tcf17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 21408
Homologene: 55921
Itpr2
Name: inositol 1,4,5-triphosphate receptor 2
Synonyms: Ip3r2, Itpr5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16439
HGNC: HGNC:6181
Homologene: 37593
Tnr
Name: tenascin R
Synonyms: janusin, restrictin, TN-R, J1-tenascin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 21960
Homologene: 124416
Nlrp9a
Name: NLR family, pyrin domain containing 9A
Synonyms: D7Ertd565e, Nalp9a, Nalp-theta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233001
Homologene: 116072
9930021J03Rik
Name: RIKEN cDNA 9930021J03 gene
Synonyms: Gm9832
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 240613
Homologene: 19046
Lrp1b
Name: low density lipoprotein-related protein 1B
Synonyms: 9630004P12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 94217
HGNC: HGNC:6693
Homologene: 56810
Ephb6
Name: Eph receptor B6
Synonyms: Mep, Cekl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 13848
HGNC: HGNC:3396
Homologene: 20940
Pcdh10
Name: protocadherin 10
Synonyms: 6430703F07Rik, 6430521D13Rik, OL-pc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 18526
Homologene: 74967
Nlrc5
Name: NLR family, CARD domain containing 5
Synonyms: AI451557
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 434341
Homologene: 88935
Csmd3
Name: CUB and Sushi multiple domains 3
Synonyms: 4930500N14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239420
Homologene: 65982
Otoa
Name: otoancorin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 246190
Homologene: 71803
Capn8
Name: calpain 8
Synonyms: nCL-2', nCL-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 170725
HGNC: HGNC:1485
Homologene: 15643
Ugt2b38
Name: UDP glucuronosyltransferase 2 family, polypeptide B38
Synonyms: 9430041C03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100559
Homologene: 137225
Masp1
Name: mannan-binding lectin serine peptidase 1
Synonyms: Crarf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 17174
VEGA: 16
HGNC: HGNC:6901
Homologene: 88793
Pdzrn3
Name: PDZ domain containing RING finger 3
Synonyms: semaphorin cytoplasmic domain-associated protein 3A, 1110020C07Rik, LNX3, Semcap3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 55983
Homologene: 10328
Ubtfl1
Name: upstream binding transcription factor, RNA polymerase I-like 1
Synonyms: B020006M18Rik, Hmgpi
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 546118
Homologene: 110115
Axl
Name: AXL receptor tyrosine kinase
Synonyms: Tyro7, Ark, Ufo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 26362
HGNC: HGNC:905
Homologene: 7583
Gcc2
Name: GRIP and coiled-coil domain containing 2
Synonyms: 0610043A03Rik, 2210420P05Rik, 2600014C01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 70297
Homologene: 45639
Gm7008
Name: predicted gene 7008
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 629820
Scn11a
Name: sodium channel, voltage-gated, type XI, alpha
Synonyms: NaT, SNS2, NaN, NSS2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 24046
VEGA: 9
Homologene: 8041
Nlrp5
Name: NLR family, pyrin domain containing 5
Synonyms: Op1, Mater, Nalp5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 23968
Homologene: 65105
Kbtbd12
Name: kelch repeat and BTB (POZ) domain containing 12
Synonyms: 4833415F11Rik, 4933428M03Rik, Klhdc6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 74589
Homologene: 52613
Fmn1
Name: formin 1
Synonyms: Fmn, formin-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14260
HGNC: HGNC:3768
Homologene: 121778
Rhag
Name: Rhesus blood group-associated A glycoprotein
Synonyms: Rh50A, Rh50, CD241
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 19743
Homologene: 68045
Olfr1058
Name: olfactory receptor 1058
Synonyms: GA_x6K02T2Q125-47855818-47854868, MOR190-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258386
Homologene: 74093
Slc22a2
Name: solute carrier family 22 (organic cation transporter), member 2
Synonyms: Oct2, Orct2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 20518
VEGA: 17
Homologene: 68293
Rftn1
Name: raftlin lipid raft linker 1
Synonyms: 2310015N21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 76438
Homologene: 18745
Dtx3l
Name: deltex 3-like, E3 ubiquitin ligase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 209200
Homologene: 51375
Sgms2
Name: sphingomyelin synthase 2
Synonyms: 4933405A16Rik, 5133401H06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 74442
Homologene: 32705
Stx19
Name: syntaxin 19
Synonyms: A030009B12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 68159
Homologene: 55777
Gatd3a
Name: glutamine amidotransferase like class 1 domain containing 3A
Synonyms: D10Jhu81e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 28295
VEGA: 10
Homologene: 3416
Atp10d
Name: ATPase, class V, type 10D
Synonyms: IMAGE:1069176, D5Buc24e, 9830145H18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231287
Gm10499
Name: predicted gene 10499
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 69717
HGNC: HGNC:4931
Homologene: 128352
Gm13054
Name: predicted gene 13054
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Gm11505
Name: predicted gene 11505
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 13,578,125 bp
  • A to G, chromosome 1 at 159,923,315 bp
  • A to G, chromosome 1 at 161,834,705 bp
  • A to C, chromosome 1 at 182,596,989 bp
  • A to T, chromosome 1 at 193,355,034 bp
  • G to A, chromosome 2 at 41,110,840 bp
  • A to G, chromosome 2 at 86,385,849 bp
  • G to T, chromosome 2 at 102,113,609 bp
  • A to G, chromosome 2 at 113,365,125 bp
  • A to G, chromosome 2 at 119,923,453 bp
  • A to G, chromosome 3 at 45,392,814 bp
  • G to A, chromosome 3 at 88,297,225 bp
  • T to A, chromosome 3 at 131,342,117 bp
  • A to T, chromosome 4 at 48,279,435 bp
  • T to C, chromosome 4 at 148,001,316 bp
  • C to T, chromosome 5 at 72,269,326 bp
  • A to G, chromosome 5 at 87,423,742 bp
  • C to T, chromosome 6 at 41,614,261 bp
  • T to A, chromosome 6 at 88,617,699 bp
  • C to T, chromosome 6 at 101,153,311 bp
  • T to G, chromosome 6 at 116,209,004 bp
  • T to A, chromosome 6 at 146,166,651 bp
  • T to A, chromosome 7 at 23,417,976 bp
  • G to A, chromosome 7 at 25,745,175 bp
  • G to A, chromosome 7 at 25,760,807 bp
  • T to C, chromosome 7 at 25,786,944 bp
  • A to G, chromosome 7 at 26,557,292 bp
  • T to C, chromosome 7 at 49,551,725 bp
  • A to G, chromosome 7 at 121,139,793 bp
  • T to A, chromosome 7 at 141,793,971 bp
  • G to T, chromosome 8 at 94,494,316 bp
  • G to T, chromosome 9 at 18,410,030 bp
  • T to A, chromosome 9 at 45,104,266 bp
  • T to A, chromosome 9 at 119,754,792 bp
  • A to G, chromosome 10 at 20,987,022 bp
  • T to A, chromosome 10 at 58,286,160 bp
  • A to G, chromosome 10 at 78,163,533 bp
  • CGTGACCTTTCTGGT to CGT, chromosome 10 at 85,645,522 bp
  • A to G, chromosome 10 at 108,261,025 bp
  • T to A, chromosome 11 at 51,070,049 bp
  • T to A, chromosome 11 at 58,386,808 bp
  • T to C, chromosome 11 at 87,796,457 bp
  • T to C, chromosome 12 at 40,223,343 bp
  • T to A, chromosome 12 at 78,590,587 bp
  • A to G, chromosome 13 at 108,384,820 bp
  • T to A, chromosome 13 at 112,851,512 bp
  • T to C, chromosome 15 at 47,888,684 bp
  • A to T, chromosome 15 at 91,928,025 bp
  • G to T, chromosome 16 at 23,494,695 bp
  • A to G, chromosome 16 at 35,938,793 bp
  • T to G, chromosome 16 at 62,822,574 bp
  • A to G, chromosome 17 at 12,586,832 bp
  • G to A, chromosome 17 at 36,143,339 bp
  • T to C, chromosome 17 at 40,828,504 bp
  • A to G, chromosome 17 at 50,107,219 bp
  • T to C, chromosome 18 at 12,933,696 bp
  • T to A, chromosome 19 at 8,910,591 bp
  • A to T, chromosome 19 at 29,719,050 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5224 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042797-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.