Strain Name:
C57BL/6J-MtgxR5231Btlr/Mmmh
Stock Number:
042803-MU
Citation ID:
RRID:MMRRC_042803-MU
Other Names:
R5231 (G1), C57BL/6J-MtgxR5231Btlr
Major Collection:

Gene Information

Kmt2c
Name: lysine (K)-specific methyltransferase 2C
Synonyms: Mll3, E330008K23Rik, HALR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231051
Homologene: 46480
Frem2
Name: Fras1 related extracellular matrix protein 2
Synonyms: b2b1562Clo, my, 8430406N05Rik, 6030440P17Rik, ne
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 242022
Homologene: 18454
Mmp10
Name: matrix metallopeptidase 10
Synonyms: stromelysin 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 17384
VEGA: 9
HGNC: HGNC:7156
Homologene: 20546
Mycbp2
Name: MYC binding protein 2, E3 ubiquitin protein ligase
Synonyms: Phr1, C130061D10Rik, Pam
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 105689
Homologene: 9005
Trip11
Name: thyroid hormone receptor interactor 11
Synonyms: GMAP-210, 3110031G15Rik, 2610511G22Rik, 6030460N08Rik, TRIP230
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 109181
Homologene: 20897
Vps8
Name: VPS8 CORVET complex subunit
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 209018
Homologene: 44592
Rhot1
Name: ras homolog family member T1
Synonyms: 2210403N23Rik, FLJ11040, Miro1, Arht1, C430039G08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 59040
Homologene: 56803
Mfsd2a
Name: major facilitator superfamily domain containing 2A
Synonyms: Mfsd2, 1700018O18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 76574
Homologene: 19229
Peg10
Name: paternally expressed 10
Synonyms: Rtl2, Mart2, Edr, MyEF-3 like, MyEF-3, HB-1, MEF3L, Mar2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 170676
Homologene: 116067
Isl1
Name: ISL1 transcription factor, LIM/homeodomain
Synonyms: Islet 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 16392
HGNC: HGNC:6132
Homologene: 1661
Csk
Name: c-src tyrosine kinase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12988
VEGA: 9
HGNC: HGNC:2444
Homologene: 55818
4931408C20Rik
Name: RIKEN cDNA 4931408C20 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 210940
Homologene: 86827
Deup1
Name: deuterosome assembly protein 1
Synonyms: Ccdc67, 4933401K09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 234964
Homologene: 18760
Bche
Name: butyrylcholinesterase
Synonyms: C730038G20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 12038
HGNC: HGNC:983
Homologene: 20065
Rnpepl1
Name: arginyl aminopeptidase (aminopeptidase B)-like 1
Synonyms: 1110014H17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 108657
Homologene: 36367
Olfr394
Name: olfactory receptor 394
Synonyms: MOR135-8, GA_x6K02T2P1NL-4043306-4042374
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 259009
Homologene: 133669
Col6a4
Name: collagen, type VI, alpha 4
Synonyms: EG235580, Vwa6, 1110001D15Rik, Dvwa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 68553
Homologene: 130754
Stat2
Name: signal transducer and activator of transcription 2
Synonyms: 1600010G07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 20847
VEGA: 10
Homologene: 3952
Lhcgr
Name: luteinizing hormone/choriogonadotropin receptor
Synonyms: LH-R, Lhr, Gpcr19-rs1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 16867
VEGA: 17
HGNC: HGNC:6585
Homologene: 37276
Ltb
Name: lymphotoxin B
Synonyms: lymphotoxin beta, p33, Tnfsf3, Tnfc, LTbeta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 16994
HGNC: HGNC:6711
Homologene: 1752
Csmd2
Name: CUB and Sushi multiple domains 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 329942
Homologene: 89034
Vmn2r37
Name: vomeronasal 2, receptor 37
Synonyms: V2r14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 22305
Homologene: 113703
Igfn1
Name: immunoglobulin-like and fibronectin type III domain containing 1
Synonyms: 9830123M21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226438
Homologene: 130054
Chil3
Name: chitinase-like 3
Synonyms: Ym1, Chi3l3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 12655
Homologene: 74931
Trim47
Name: tripartite motif-containing 47
Synonyms: 2210023F24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217333
Homologene: 14128
Olfr1499
Name: olfactory receptor 1499
Synonyms: MOR211-2, GA_x6K02T2RE5P-4147744-4146800
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 258792
Homologene: 17393
Vmn2r60
Name: vomeronasal 2, receptor 60
Synonyms: EG637898, Casr-rs3, Gprc2a-rs3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 637898
Homologene: 129683
Plscr1
Name: phospholipid scramblase 1
Synonyms: Tras2, MuPLSCR2, Tras1, MmTRA1b, MmTRA1a, NOR1, TRA1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 22038
HGNC: HGNC:9092
Homologene: 41418
Olfr181
Name: olfactory receptor 181
Synonyms: GA_x54KRFPKG5P-55145984-55145034, MOR184-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 259001
Homologene: 74112
Ttll1
Name: tubulin tyrosine ligase-like 1
Synonyms: 6330444E16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 319953
HGNC: HGNC:1312
Homologene: 8174
Klk1b16
Name: kallikrein 1-related peptidase b16
Synonyms: mGk-16, Klk16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 16615
HGNC: HGNC:6364
Homologene: 68141
Zfp354b
Name: zinc finger protein 354B
Synonyms: Kid2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 27274
Homologene: 32187
9130019O22Rik
Name: RIKEN cDNA 9130019O22 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 78921
Homologene: 118005
Adat3
Name: adenosine deaminase, tRNA-specific 3
Synonyms: A430024H01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 100113398
VEGA: 10
Homologene: 50671
Olfr589
Name: olfactory receptor 589
Synonyms: GA_x6K02T2PBJ9-5871256-5870303, MOR32-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 259054
Homologene: 133046
Ighv1-53
Name: immunoglobulin heavy variable 1-53
Synonyms: V23-D-J-C mu, AB069917
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 780931
Igkv2-109
Name: immunoglobulin kappa variable 2-109
Synonyms: ENSMUSG00000060986
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 628268
Ear-ps2
Name: eosinophil-associated, ribonuclease A family, pseudogene 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 93743
Ighv1-62-3
Name: immunoglobulin heavy variable 1-62-3
Synonyms: Gm9235
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 668549
Rpl15-ps6
Name: ribosomal protein L15, pseudogene 6
Synonyms: Gm10020
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 100039748
VEGA: 15
Pdcd5-ps
Name: programmed cell death 5, pseudogene
Synonyms: Gm37810, Gm3837
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 100042424
HGNC: HGNC:8764
Homologene: 10506
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 26,683,951 bp
  • G to T, chromosome 1 at 92,911,270 bp
  • T to C, chromosome 1 at 135,966,736 bp
  • T to C, chromosome 1 at 189,277,652 bp
  • T to C, chromosome 3 at 53,522,295 bp
  • G to A, chromosome 3 at 73,700,861 bp
  • A to G, chromosome 3 at 106,155,729 bp
  • A to G, chromosome 4 at 122,959,301 bp
  • A to G, chromosome 4 at 128,546,049 bp
  • A to T, chromosome 5 at 25,315,473 bp
  • C to T, chromosome 6 at 4,756,939 bp
  • T to A, chromosome 6 at 68,302,445 bp
  • C to A, chromosome 7 at 9,206,595 bp
  • A to T, chromosome 7 at 42,137,024 bp
  • T to A, chromosome 7 at 44,137,347 bp
  • A to T, chromosome 7 at 103,154,968 bp
  • T to C, chromosome 7 at 127,385,414 bp
  • T to C, chromosome 9 at 7,502,500 bp
  • G to T, chromosome 9 at 15,575,199 bp
  • G to T, chromosome 9 at 57,630,378 bp
  • C to A, chromosome 9 at 92,266,731 bp
  • T to C, chromosome 9 at 106,025,531 bp
  • T to C, chromosome 10 at 80,606,426 bp
  • T to C, chromosome 10 at 128,281,242 bp
  • A to C, chromosome 11 at 50,923,090 bp
  • C to A, chromosome 11 at 73,887,955 bp
  • T to C, chromosome 11 at 80,227,334 bp
  • C to A, chromosome 11 at 116,106,622 bp
  • T to C, chromosome 12 at 101,885,601 bp
  • C to A, chromosome 12 at 115,158,605 bp
  • A to T, chromosome 12 at 115,461,051 bp
  • T to C, chromosome 13 at 116,301,657 bp
  • G to A, chromosome 14 at 44,047,060 bp
  • A to C, chromosome 14 at 103,346,214 bp
  • T to C, chromosome 15 at 52,478,044 bp
  • T to A, chromosome 15 at 83,489,466 bp
  • A to T, chromosome 16 at 21,576,725 bp
  • T to C, chromosome 16 at 58,925,714 bp
  • C to T, chromosome 17 at 35,195,826 bp
  • T to A, chromosome 17 at 88,755,611 bp
  • A to T, chromosome 19 at 13,815,347 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5231 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042803-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.