Strain Name:
C57BL/6J-MtgxR5270Btlr/Mmmh
Stock Number:
042835-MU
Citation ID:
RRID:MMRRC_042835-MU
Other Names:
R5270 (G1), C57BL/6J-MtgxR5270Btlr
Major Collection:

Gene Information

Msx2
Name: msh homeobox 2
Synonyms: Hox8.1, Hox8, Hox-8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 17702
HGNC: HGNC:7392
Homologene: 1837
Adamts20
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 20
Synonyms: ADAMTS-20, bt
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223838
Homologene: 11808
Epha4
Name: Eph receptor A4
Synonyms: Sek, Sek1, Tyro1, Hek8, Cek8, 2900005C20Rik, rb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 13838
HGNC: HGNC:3388
Homologene: 20933
Zfa-ps
Name: zinc finger protein, autosomal, pseudogene
Synonyms: Zfa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 22639
Prkdc
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: DNA-PKcs, slip, DNAPDcs, XRCC7, DNA-PK, DOXNPH, dxnph
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 19090
HGNC: HGNC:9413
Homologene: 5037
Trim6
Name: tripartite motif-containing 6
Synonyms: D7Ertd684e, C430046K18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 94088
Homologene: 14381
Atpif1
Name: ATPase inhibitory factor 1
Synonyms: If1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 11983
HGNC: HGNC:871
Homologene: 40581
Zc3h4
Name: zinc finger CCCH-type containing 4
Synonyms: LOC330474, Bwq1, Kiaa1064-hp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 330474
Homologene: 87150
Akap9
Name: A kinase (PRKA) anchor protein (yotiao) 9
Synonyms: AKAP450, 5730481H23Rik, G1-448-15, repro12, mei2-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100986
HGNC: HGNC:379
Homologene: 134583
Dock4
Name: dedicator of cytokinesis 4
Synonyms: EST N28122, 6330411N01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 238130
VEGA: 12
Homologene: 56680
C77080
Name: expressed sequence C77080
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 97130
Homologene: 45837
Bdp1
Name: B double prime 1, subunit of RNA polymerase III transcription initiation factor IIIB
Synonyms: TFIIIB150, TFIIIB90, TAF3B1, TFC5, Tfnr, G630013P12Rik, B130055N23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 544971
Homologene: 34582
Smtnl2
Name: smoothelin-like 2
Synonyms: D130058I21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 276829
Homologene: 82367
M6pr
Name: mannose-6-phosphate receptor, cation dependent
Synonyms: Mpr46, CD-MPR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 17113
HGNC: HGNC:6752
Homologene: 31086
Zfp146
Name: zinc finger protein 146
Synonyms: OZF
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 26465
Homologene: 117615
Mmp17
Name: matrix metallopeptidase 17
Synonyms: MT4-MMP, membrane type-4 matrix metalloproteinase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 23948
HGNC: HGNC:7163
Homologene: 22669
Csf2rb2
Name: colony stimulating factor 2 receptor, beta 2, low-affinity (granulocyte-macrophage)
Synonyms: AIC2A, Il3r, Il3rb2, Bil3, BetaIl3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 12984
VEGA: 15
HGNC: HGNC:2436
Homologene: 339
Kdm3b
Name: KDM3B lysine (K)-specific demethylase 3B
Synonyms: 5830462I21Rik, JHDM2B, Jmjd1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 277250
VEGA: 18
HGNC: HGNC:1337
Homologene: 41145
Epg5
Name: ectopic P-granules autophagy protein 5 homolog (C. elegans)
Synonyms: 5430411K18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 100502841
VEGA: 18
Homologene: 14575
Acsl5
Name: acyl-CoA synthetase long-chain family member 5
Synonyms: 1700030F05Rik, Facl5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 433256
VEGA: 19
Homologene: 69208
Per1
Name: period circadian clock 1
Synonyms: m-rigui, mPer1, Hftm
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18626
HGNC: HGNC:8845
Homologene: 1966
Ints2
Name: integrator complex subunit 2
Synonyms: 2810417D08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 70422
Homologene: 10801
Saal1
Name: serum amyloid A-like 1
Synonyms: 5031425D22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 78935
Homologene: 34706
Hcrt
Name: hypocretin
Synonyms: orexin A, orexin B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 15171
HGNC: HGNC:4847
Homologene: 1166
Crb1
Name: crumbs family member 1, photoreceptor morphogenesis associated
Synonyms: A930008G09Rik, 7530426H14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 170788
HGNC: HGNC:2343
Homologene: 8092
Pkdrej
Name: polycystin (PKD) family receptor for egg jelly
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 18766
VEGA: 15
HGNC: HGNC:9015
Homologene: 4427
Wdr17
Name: WD repeat domain 17
Synonyms: 3010002I12Rik, B230207L18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244484
Homologene: 12460
Kcns1
Name: K+ voltage-gated channel, subfamily S, 1
Synonyms: Kv9.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16538
HGNC: HGNC:6300
Homologene: 20517
Gpr35
Name: G protein-coupled receptor 35
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 64095
HGNC: HGNC:4492
Homologene: 3874
Ciz1
Name: CDKN1A interacting zinc finger protein 1
Synonyms: 0610038H21Rik, 2900056O04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 68379
Homologene: 8112
Henmt1
Name: HEN1 methyltransferase homolog 1 (Arabidopsis)
Synonyms: Hen1, 4921515J06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 66715
Homologene: 32638
Dpp6
Name: dipeptidylpeptidase 6
Synonyms: Dpp-6, Peplb, In(5)6H-p, Rw, LOC384168, B930011P16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 13483
HGNC: HGNC:3010
Homologene: 22560
Irs2
Name: insulin receptor substrate 2
Synonyms: Irs-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 384783
HGNC: HGNC:6126
Homologene: 2778
Tbc1d9
Name: TBC1 domain family, member 9
Synonyms: C76116, 4933431N12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 71310
Homologene: 57079
Zfp607a
Name: zinc finger protein 607A
Synonyms: 4732475C15Rik, Zfp607
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 545938
Homologene: 134321
Adam6a
Name: a disintegrin and metallopeptidase domain 6A
Synonyms: Adam6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 238406
HGNC: HGNC:213
Homologene: 128362
Vmn2r104
Name: vomeronasal 2, receptor 104
Synonyms: V2r7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 22313
Homologene: 129751
Sdcbp2
Name: syndecan binding protein (syntenin) 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228765
Homologene: 9240
Gabrb1
Name: gamma-aminobutyric acid (GABA) A receptor, subunit beta 1
Synonyms: Gabrb-1, B230208N19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 14400
HGNC: HGNC:4081
Homologene: 20221
Sntb1
Name: syntrophin, basic 1
Synonyms: beta1-Syntrophin, 59-1 DAP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 20649
Homologene: 9618
Spag4
Name: sperm associated antigen 4
Synonyms: 1700041K21Rik, Sun4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 245865
Homologene: 2343
Prdm9
Name: PR domain containing 9
Synonyms: Meisetz, G1-419-29, repro7, Dsbc1, Rcr1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 213389
Homologene: 104139
Tmem163
Name: transmembrane protein 163
Synonyms: 2610024A01Rik, SV31
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 72160
Homologene: 12798
Pramel27
Name: PRAME like 27
Synonyms: Gm13103
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 194225
Homologene: 129883
Rasgrf1
Name: RAS protein-specific guanine nucleotide-releasing factor 1
Synonyms: CDC25Mm, Grfbeta, CDC25, Grf1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 19417
HGNC: HGNC:9875
Homologene: 74972
Tm9sf1
Name: transmembrane 9 superfamily member 1
Synonyms: MP70, 1200014D02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 74140
Homologene: 4671
Skida1
Name: SKI/DACH domain containing 1
Synonyms: 5730507N06Rik, 2810030E01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 72668
Homologene: 66327
Vmn1r47
Name: vomeronasal 1 receptor 47
Synonyms: V1ra4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 113846
Homologene: 137651
Hrh1
Name: histamine receptor H1
Synonyms: Bphs, Hir
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 15465
HGNC: HGNC:5182
Homologene: 668
Cyp3a25
Name: cytochrome P450, family 3, subfamily a, polypeptide 25
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 56388
Homologene: 135775
Macir
Name: macrophage immunometabolism regulator
Synonyms: D1Ertd622e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 52392
Homologene: 13239
Ly9
Name: lymphocyte antigen 9
Synonyms: T100, Lgp100, CD229, SLAMF3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 17085
HGNC: HGNC:6730
Homologene: 1759
Zfp353-ps
Name: zinc finger protein 353, pseudogene
Synonyms: Zfp353
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234203
Rrh
Name: retinal pigment epithelium derived rhodopsin homolog
Synonyms: Peropsin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 20132
Homologene: 55977
Nit2
Name: nitrilase family, member 2
Synonyms: 1190017B19Rik, D16Ertd502e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 52633
VEGA: 16
Homologene: 6520
B230104I21Rik
Name: RIKEN cDNA B230104I21 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 77,506,607 bp
  • A to T, chromosome 1 at 92,982,577 bp
  • T to C, chromosome 1 at 97,645,995 bp
  • G to A, chromosome 1 at 127,491,552 bp
  • T to A, chromosome 1 at 139,236,864 bp
  • T to C, chromosome 1 at 171,601,162 bp
  • C to A, chromosome 2 at 18,047,649 bp
  • C to A, chromosome 2 at 32,374,499 bp
  • A to G, chromosome 2 at 151,584,892 bp
  • G to T, chromosome 2 at 156,065,933 bp
  • C to A, chromosome 2 at 164,168,329 bp
  • G to A, chromosome 3 at 108,960,214 bp
  • T to C, chromosome 3 at 129,813,349 bp
  • T to C, chromosome 4 at 129,224,212 bp
  • A to G, chromosome 4 at 132,533,300 bp
  • A to G, chromosome 4 at 143,851,898 bp
  • A to G, chromosome 4 at 154,349,593 bp
  • T to C, chromosome 5 at 3,954,399 bp
  • C to T, chromosome 5 at 27,634,534 bp
  • A to T, chromosome 5 at 72,108,326 bp
  • G to A, chromosome 5 at 129,601,933 bp
  • A to T, chromosome 5 at 145,981,502 bp
  • A to T, chromosome 6 at 90,022,543 bp
  • C to A, chromosome 6 at 114,481,218 bp
  • G to A, chromosome 6 at 122,315,089 bp
  • A to G, chromosome 7 at 16,434,515 bp
  • A to T, chromosome 7 at 27,878,305 bp
  • G to T, chromosome 7 at 30,162,475 bp
  • G to T, chromosome 7 at 46,701,733 bp
  • G to A, chromosome 7 at 104,225,812 bp
  • G to A, chromosome 8 at 11,006,678 bp
  • G to T, chromosome 8 at 42,081,535 bp
  • G to T, chromosome 8 at 54,643,186 bp
  • A to G, chromosome 8 at 83,233,654 bp
  • A to C, chromosome 9 at 90,026,694 bp
  • A to G, chromosome 10 at 52,543,456 bp
  • T to C, chromosome 11 at 69,103,598 bp
  • T to A, chromosome 11 at 72,399,917 bp
  • T to C, chromosome 11 at 86,215,795 bp
  • T to C, chromosome 11 at 100,761,997 bp
  • A to G, chromosome 12 at 40,733,271 bp
  • A to T, chromosome 12 at 113,544,127 bp
  • C to A, chromosome 13 at 53,472,537 bp
  • A to G, chromosome 13 at 100,099,097 bp
  • G to T, chromosome 14 at 55,636,481 bp
  • C to G, chromosome 15 at 55,642,795 bp
  • A to T, chromosome 15 at 78,291,982 bp
  • A to T, chromosome 15 at 85,818,327 bp
  • G to A, chromosome 15 at 94,282,519 bp
  • T to C, chromosome 16 at 15,734,955 bp
  • G to A, chromosome 16 at 57,157,131 bp
  • G to T, chromosome 17 at 15,553,363 bp
  • A to T, chromosome 17 at 20,038,266 bp
  • T to C, chromosome 18 at 34,827,414 bp
  • T to A, chromosome 18 at 77,983,563 bp
  • T to C, chromosome 19 at 55,294,218 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5270 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042835-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.