Strain Name:
C57BL/6J-MtgxR5305Btlr/Mmmh
Stock Number:
042888-MU
Citation ID:
RRID:MMRRC_042888-MU
Other Names:
R5305 (G1), C57BL/6J-MtgxR5305Btlr
Major Collection:

Strain Information

Pitx2
Name: paired-like homeodomain transcription factor 2
Synonyms: Otlx2, Brx1b, Brx1a, Brx1, solurshin, Ptx2, Pitx2c, Pitx2b, Pitx2a, Munc30, Rieg
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18741
HGNC: HGNC:9005
Homologene: 55454
Sema3b
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B
Synonyms: SemA, semaV, sema5, SemA, LUCA-1, Semaa
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20347
Homologene: 20972
Areg
Name: amphiregulin
Synonyms: AR, Sdgf, Mcub
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 11839
HGNC: HGNC:651
Homologene: 1252
Thbs3
Name: thrombospondin 3
Synonyms: Thbs-3, TSP3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 21827
Homologene: 5159
Mycbp2
Name: MYC binding protein 2, E3 ubiquitin protein ligase
Synonyms: Pam, C130061D10Rik, Phr1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 105689
Homologene: 9005
Prex2
Name: phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2
Synonyms: 6230420N16Rik, C030045D06Rik, Depdc2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 109294
Homologene: 23523
Dot1l
Name: DOT1 like histone lysine methyltransferase
Synonyms: mDot1, KMT4
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 208266
Homologene: 32779
Mrpl20
Name: mitochondrial ribosomal protein L20
Synonyms: 2610008D01Rik, 4930425I20Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 66448
Homologene: 9941
Foxj3
Name: forkhead box J3
Synonyms: C330039G02Rik, Fhd6
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230700
Homologene: 35243
Ppard
Name: peroxisome proliferator activator receptor delta
Synonyms: Nr1c2, PPARdelta/beta, Pparb, Peroxisome proliferator-activated receptor beta, NUC1, PPAR-delta, Pparb/d
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 19015
HGNC: HGNC:9235
Homologene: 4544
Auts2
Name: autism susceptibility candidate 2
Synonyms: D830032G16Rik, A730011F23Rik, 2700063G02Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 319974
Homologene: 22907
Mtf2
Name: metal response element binding transcription factor 2
Synonyms: M96, 9230112N11Rik, C76717, Pcl2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 17765
Homologene: 7207
Actb
Name: actin, beta
Synonyms: A-X actin-like protein, Actx, E430023M04Rik, beta-actin
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 11461
HGNC: HGNC:132
Homologene: 110648
Skic3
Name: SKI3 subunit of superkiller complex
Synonyms: Ttc37
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218343
VEGA: 13
Homologene: 40966
Cfap36
Name: cilia and flagella associated protein 36
Synonyms: 4931428D14Rik, Ccdc104
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216618
Homologene: 41646
Polr2e
Name: polymerase (RNA) II (DNA directed) polypeptide E
Synonyms: XAP4, RPB5, 25kDa
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 66420
HGNC: HGNC:9192
Homologene: 2018
Sf3b4
Name: splicing factor 3b, subunit 4
Synonyms: SF3b49, Sap49, 49kDa
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 107701
Homologene: 134086
Spag9
Name: sperm associated antigen 9
Synonyms: 4733401I23Rik, syd1, 4831406C20Rik, Mapk8ip4, JIP4, 3110018C07Rik, JLP
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 70834
Homologene: 2954
Wdr25
Name: WD repeat domain 25
Synonyms: B930090D16Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 212198
VEGA: 12
Homologene: 56993
Sv2a
Name: synaptic vesicle glycoprotein 2a
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 64051
Homologene: 32237
Sgta
Name: small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha
Synonyms: 5330427H01Rik, D10Ertd190e, Sgt
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 52551
VEGA: 10
Homologene: 31122
Top3a
Name: topoisomerase (DNA) III alpha
Synonyms: Top IIIa
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 21975
Homologene: 3394
Zfp574
Name: zinc finger protein 574
Synonyms: A630056B21Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 232976
Homologene: 11238
Atad2b
Name: ATPase family, AAA domain containing 2B
Synonyms: 1110014E10Rik, D530031C13Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 320817
VEGA: 12
Homologene: 86351
Kdm4a
Name: lysine (K)-specific demethylase 4A
Synonyms: Jmjd2, D4Ertd222e, JHDM3A, Jmjd2a
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230674
Homologene: 27780
Nr2e1
Name: nuclear receptor subfamily 2, group E, member 1
Synonyms: Nr2e1, Tlx, Mtll, tailless
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 21907
HGNC: HGNC:7973
Homologene: 37750
Mgat4c
Name: MGAT4 family, member C
Synonyms: 9130411I17Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 67569
Homologene: 8297
Cubn
Name: cubilin
Synonyms: D2Wsu88e, intrinsic factor-cobalamin receptor
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 65969
HGNC: HGNC:2548
Homologene: 37434
Ptprd
Name: protein tyrosine phosphatase receptor type D
Synonyms: 3000002J10Rik, B230219D21Rik, 1110002J03Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 19266
HGNC: HGNC:9668
Zfp458
Name: zinc finger protein 458
Synonyms: Rslcan-7
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 238690
VEGA: 13
Homologene: 128170
Eps8l1
Name: EPS8-like 1
Synonyms: 4632407K17Rik, EPS8R1, DRC3, 2310051G19Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 67425
Homologene: 15767
Gls2
Name: glutaminase 2 (liver, mitochondrial)
Synonyms: Lga, A330074B06Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216456
Homologene: 40861
Col6a3
Name: collagen, type VI, alpha 3
Synonyms: Col6a-3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12835
HGNC: HGNC:2213
Homologene: 37917
Pcdh10
Name: protocadherin 10
Synonyms: 6430703F07Rik, 6430521D13Rik, OL-pc, Olpc
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18526
Homologene: 74967
Rims1
Name: regulating synaptic membrane exocytosis 1
Synonyms: RIM1, RIM1a, C030033M19Rik, RIM1alpha
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 116837
Homologene: 128399
Obscn
Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Synonyms: LOC380698, OTTMUSG00000005786
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 380698
Homologene: 70869
Gxylt2
Name: glucoside xylosyltransferase 2
Synonyms: LOC232313, Glt8d4
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232313
Homologene: 16823
4930407I10Rik
Name: RIKEN cDNA 4930407I10 gene
Synonyms: LOC328573
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 328573
VEGA: 15
Homologene: 130065
Nlrc3
Name: NLR family, CARD domain containing 3
Synonyms: CLR16.2, D230007K08Rik, Caterpiller 16.2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 268857
Homologene: 18720
Erich6
Name: glutamate rich 6
Synonyms: 4932431H17Rik, Fam194a
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 545527
Homologene: 65043
Nos1ap
Name: nitric oxide synthase 1 (neuronal) adaptor protein
Synonyms: 6330408P19Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 70729
Homologene: 135990
Sytl2
Name: synaptotagmin-like 2
Synonyms: Slp2-a, Slp2, Slp2-d, Slp2-c, Slp2-b
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 83671
Homologene: 131343
Iqsec1
Name: IQ motif and Sec7 domain 1
Synonyms: cI-43, D6Ertd349e, BRAG2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232227
Homologene: 82429
Cttnbp2
Name: cortactin binding protein 2
Synonyms: ORF4, Cortbp2, 9130022E09Rik, 4732477G22Rik, 3010022N24Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 30785
Homologene: 14125
Gm7535
Name: predicted gene 7535
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 665187
VEGA: 17
Vmn1r61
Name: vomeronasal 1 receptor 61
Synonyms: Gm7186
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 636731
Homologene: 41799
Rimbp2
Name: RIMS binding protein 2
Synonyms: A930033C01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231760
Homologene: 14672
Ap1g2
Name: adaptor protein complex AP-1, gamma 2 subunit
Synonyms: gamma 2-adaptin, Adtg2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 11766
HGNC: HGNC:556
Homologene: 49141
Sry
Name: sex determining region of Chr Y
Synonyms: Tdf, Tdy
Type: Gene
Species: Mouse
Chromosome: Y
NCBI: 21674
Tyk2
Name: tyrosine kinase 2
Synonyms: JTK1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 54721
VEGA: 9
Homologene: 20712
Or7a37
Name: olfactory receptor family 7 subfamily A member 37
Synonyms: GA_x6K02T2QGN0-2842591-2841662, MOR139-2, Olfr1353
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 259044
Homologene: 131345
Brinp1
Name: bone morphogenic protein/retinoic acid inducible neural specific 1
Synonyms: Dbccr1, Fam5a, Dbc1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 56710
HGNC: HGNC:2687
Homologene: 8754
Ceacam3
Name: CEA cell adhesion molecule 3
Synonyms: cea12, EG384557, Psg24
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 384557
HGNC: HGNC:1819
Homologene: 86971
Krtap4-22
Name: keratin associated protein 4-22
Synonyms: Gm11595
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 100040276
Homologene: 124486
Asb13
Name: ankyrin repeat and SOCS box-containing 13
Synonyms: 6430573K02Rik, 2210015B19Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 142688
Homologene: 11666
Erlec1
Name: endoplasmic reticulum lectin 1
Synonyms: 4933407N01Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 66753
Homologene: 9247
Crebzf
Name: CREB/ATF bZIP transcription factor
Synonyms: LAZipII, LAZip, 1110034C16Rik, Zf, 6330417B10Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233490
Homologene: 10926
Rab3c
Name: RAB3C, member RAS oncogene family
Synonyms: 3110037E15Rik, 3110015B08Rik, 2700062I01Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 67295
VEGA: 13
Homologene: 23420
Prss30
Name: serine protease 30
Synonyms: 2010015P21Rik, Disp, Disp1, Tmprss8
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 30943
Homologene: 88826
Cdnf
Name: cerebral dopamine neurotrophic factor
Synonyms: Armetl1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227526
Homologene: 27965
Acot8
Name: acyl-CoA thioesterase 8
Synonyms: PTE-2, Pte1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 170789
Homologene: 3991
Ppp1r27
Name: protein phosphatase 1, regulatory subunit 27
Synonyms: toonin, 1110033I14Rik, Dysfip1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 68701
Homologene: 45933
Gstm2-ps1
Name: glutathione S-transferase mu 2 (muscle), pseudogene 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 433943
Gm12854
Name: predicted gene 12854
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 102637129
Homologene: 137318
Zscan4c
Name: zinc finger and SCAN domain containing 4C
Synonyms: LOC245109
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 245109
Homologene: 85986
Gm28529
Name: predicted gene 28529
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 11,107,678 bp
  • T to A, chromosome 1 at 22,596,542 bp
  • G to C, chromosome 1 at 90,801,531 bp
  • T to A, chromosome 1 at 170,349,399 bp
  • A to G, chromosome 2 at 3,519,347 bp
  • C to A, chromosome 2 at 13,388,939 bp
  • A to G, chromosome 2 at 164,795,765 bp
  • A to G, chromosome 3 at 45,392,660 bp
  • T to G, chromosome 3 at 58,625,116 bp
  • T to A, chromosome 3 at 89,217,976 bp
  • G to C, chromosome 3 at 96,173,642 bp
  • A to G, chromosome 3 at 96,185,458 bp
  • T to G, chromosome 3 at 129,215,840 bp
  • C to T, chromosome 4 at 68,798,818 bp
  • T to C, chromosome 4 at 75,982,626 bp
  • A to G, chromosome 4 at 116,067,284 bp
  • T to C, chromosome 4 at 118,160,501 bp
  • T to A, chromosome 4 at 119,619,958 bp
  • G to A, chromosome 4 at 155,803,705 bp
  • G to T, chromosome 5 at 91,144,449 bp
  • A to G, chromosome 5 at 108,104,499 bp
  • T to A, chromosome 5 at 116,633,465 bp
  • A to G, chromosome 5 at 128,797,381 bp
  • T to G, chromosome 5 at 131,443,794 bp
  • A to G, chromosome 5 at 142,904,230 bp
  • G to T, chromosome 6 at 18,381,098 bp
  • G to A, chromosome 6 at 90,662,878 bp
  • T to G, chromosome 6 at 100,787,218 bp
  • A to G, chromosome 7 at 4,477,896 bp
  • A to G, chromosome 7 at 5,610,815 bp
  • G to A, chromosome 7 at 11,009,535 bp
  • T to A, chromosome 7 at 17,151,576 bp
  • T to A, chromosome 7 at 25,081,090 bp
  • A to T, chromosome 7 at 42,613,478 bp
  • A to G, chromosome 7 at 90,381,863 bp
  • T to C, chromosome 7 at 90,444,134 bp
  • T to C, chromosome 9 at 21,109,381 bp
  • T to C, chromosome 9 at 107,603,337 bp
  • A to T, chromosome 10 at 42,571,487 bp
  • A to T, chromosome 10 at 78,970,556 bp
  • A to G, chromosome 10 at 80,038,063 bp
  • C to T, chromosome 10 at 80,790,793 bp
  • T to A, chromosome 10 at 81,046,247 bp
  • T to A, chromosome 10 at 102,389,279 bp
  • T to G, chromosome 10 at 128,204,709 bp
  • G to T, chromosome 11 at 29,244,068 bp
  • T to C, chromosome 11 at 30,943,771 bp
  • T to C, chromosome 11 at 59,012,715 bp
  • A to T, chromosome 11 at 60,762,539 bp
  • C to A, chromosome 11 at 94,069,012 bp
  • G to A, chromosome 11 at 99,772,555 bp
  • A to G, chromosome 11 at 120,550,917 bp
  • C to T, chromosome 12 at 4,965,855 bp
  • C to A, chromosome 12 at 109,026,440 bp
  • A to T, chromosome 13 at 3,643,479 bp
  • T to C, chromosome 13 at 67,256,318 bp
  • G to A, chromosome 13 at 76,147,767 bp
  • T to A, chromosome 13 at 110,181,077 bp
  • A to G, chromosome 14 at 55,099,076 bp
  • A to C, chromosome 14 at 103,346,321 bp
  • G to A, chromosome 15 at 82,059,219 bp
  • A to G, chromosome 16 at 3,949,429 bp
  • T to C, chromosome 17 at 17,911,799 bp
  • T to G, chromosome 17 at 23,972,776 bp
  • A to G, chromosome 17 at 28,298,858 bp
  • G to A, chromosome 18 at 6,490,690 bp
  • T to A, chromosome Y at 2,662,982 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5305 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042888-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.