Strain Name:
C57BL/6J-MtgxR5350Btlr/Mmmh
Stock Number:
042929-MU
Citation ID:
RRID:MMRRC_042929-MU
Other Names:
R5350 (G1), C57BL/6J-MtgxR5350Btlr
Major Collection:

Strain Information

Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Cops4
Name: COP9 signalosome subunit 4
Synonyms: COP9 complex S4, D5Ertd774e
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 26891
Homologene: 8067
Cdk13
Name: cyclin dependent kinase 13
Synonyms: 2310015O17Rik, Cdc2l5
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 69562
VEGA: 13
HGNC: HGNC:1733
Homologene: 135707
Nbea
Name: neurobeachin
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 26422
HGNC: HGNC:7648
Homologene: 69190
Rps6kc1
Name: ribosomal protein S6 kinase polypeptide 1
Synonyms: RPK118, B130003F20Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 320119
Homologene: 8244
Ell
Name: elongation factor RNA polymerase II
Synonyms: Men, Ell1, eleven-nineteen lysine-rich leukemia gene
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 13716
Homologene: 4762
Evi5
Name: ecotropic viral integration site 5
Synonyms: NB4S
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14020
HGNC: HGNC:3501
Homologene: 121902
Dusp5
Name: dual specificity phosphatase 5
Synonyms: LOC240672
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 240672
HGNC: HGNC:3071
Homologene: 3256
Bag1
Name: BCL2-associated athanogene 1
Synonyms: Rap46
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 12017
HGNC: HGNC:937
Homologene: 3190
Icam1
Name: intercellular adhesion molecule 1
Synonyms: CD54, MALA-2, Icam-1, Ly-47
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 15894
VEGA: 9
HGNC: HGNC:5344
Homologene: 168
Capns1
Name: calpain, small subunit 1
Synonyms: Capa-4, Capa4, Capn4, D7Ertd146e
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 12336
HGNC: HGNC:1481
Homologene: 1327
Acaca
Name: acetyl-Coenzyme A carboxylase alpha
Synonyms: acetyl-CoA carboxylase, Acc1, LOC327983, Acac, A530025K05Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 107476
HGNC: HGNC:84
Homologene: 31015
Ptprs
Name: protein tyrosine phosphatase receptor type S
Synonyms: PTP-NU3, PTPsigma, RPTPsigma, Ptpt9
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 19280
HGNC: HGNC:9681
Homologene: 20626
Ppip5k2
Name: diphosphoinositol pentakisphosphate kinase 2
Synonyms: Vip2, Hisppd1, Cfap160
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 227399
Homologene: 49409
Svil
Name: supervillin
Synonyms: B430302E16Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225115
Homologene: 25090
Gemin5
Name: gem nuclear organelle associated protein 5
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216766
Homologene: 9155
Atp2b2
Name: ATPase, Ca++ transporting, plasma membrane 2
Synonyms: PMCA2, D6Abb2e, wms, jog, Tmy, Gena300
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 11941
HGNC: HGNC:815
Homologene: 56150
Nr4a2
Name: nuclear receptor subfamily 4, group A, member 2
Synonyms: HZF-3, RNR-1, Nurr1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18227
HGNC: HGNC:7981
Homologene: 4509
Grn
Name: granulin
Synonyms: acrogranulin, epithelin, PC cell-derived growth factor, progranulin, Pgrn
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14824
HGNC: HGNC:4601
Homologene: 1577
Dach1
Name: dachshund family transcription factor 1
Synonyms: Dac, E130112M23Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 13134
HGNC: HGNC:2663
Homologene: 7288
Ttn
Name: titin
Synonyms: connectin, shru, D330041I19Rik, 2310057K23Rik, mdm, D830007G01Rik, 2310074I15Rik, 1100001C23Rik, 2310036G12Rik, L56
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Arap3
Name: ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3
Synonyms: DRAG1, E030006K04Rik, Centd3
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 106952
VEGA: 18
Homologene: 11199
Dnah2
Name: dynein, axonemal, heavy chain 2
Synonyms: D330014H01Rik, 2900022L05Rik, Dnhd3, Dnahc2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 327954
HGNC: HGNC:2948
Homologene: 72110
Acacb
Name: acetyl-Coenzyme A carboxylase beta
Synonyms: Accb, Acc2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100705
HGNC: HGNC:85
Homologene: 74382
Glce
Name: glucuronyl C5-epimerase
Synonyms: heparan sulfate-glucuronic acid C5-epimerase, Hsepi, C130034A12Rik, 1110017N23Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 93683
Homologene: 14111
Pcnx4
Name: pecanex homolog 4
Synonyms: 1810048J11Rik, Pcnxl4
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 67708
VEGA: 12
Homologene: 23366
Nav3
Name: neuron navigator 3
Synonyms: Pomfil1p, POMFIL1, 4732483H20Rik, unc53H3, steerin 3, 9630020C08Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 260315
Homologene: 56688
Ppargc1b
Name: peroxisome proliferative activated receptor, gamma, coactivator 1 beta
Synonyms: PGC-1beta/ERRL1, 4631412G21Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 170826
Homologene: 15776
Or7e169
Name: olfactory receptor family 7 subfamily E member 169
Synonyms: GA_x6K02T2PVTD-13586614-13585661, MOR146-2, Olfr860
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258521
Homologene: 134093
Adamts16
Name: ADAM metallopeptidase with thrombospondin type 1 motif 16
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 271127
Homologene: 15146
Stx2
Name: syntaxin 2
Synonyms: Syn-2, repro34, G1-536-1, Epim
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 13852
HGNC: HGNC:3403
Homologene: 37559
Abca9
Name: ATP-binding cassette, sub-family A member 9
Synonyms: D630040K07Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217262
HGNC: HGNC:39
Homologene: 33332
Psd3
Name: pleckstrin and Sec7 domain containing 3
Synonyms: 4931420C21Rik, EFA6D
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234353
Homologene: 87257
Jag2
Name: jagged 2
Synonyms: Serh, D12Ggc2e
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 16450
VEGA: 12
HGNC: HGNC:6189
Homologene: 1677
Or52s1b
Name: olfactory receptor family 52 subfamily S member 1B
Synonyms: GA_x6K02T2PBJ9-5889409-5888465, MOR24-1P, Olfr591
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258139
Homologene: 79429
Stk-ps2
Name: serine/threonine kinase 2
Synonyms: Gm4776
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 212225
Atg2a
Name: autophagy related 2A
Synonyms: 1810013C15Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 329015
Homologene: 86985
Disp2
Name: dispatched RND transporter family member 2
Synonyms: DispB, B230210L08Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 214240
Homologene: 24916
Dnah7b
Name: dynein, axonemal, heavy chain 7B
Synonyms: LOC227058, Dnahc7b
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 227058
Homologene: 41287
Tyms
Name: thymidylate synthase
Synonyms: TS
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 22171
Homologene: 834
H2-Q10
Name: histocompatibility 2, Q region locus 10
Synonyms: H-2Q10, Qa10, Q10
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 15007
Homologene: 128352
Or2v2
Name: olfactory receptor family 2 subfamily V member 2
Synonyms: GA_x6K02T2QP88-6321048-6321995, MOR276-2, Olfr1396
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 258334
Homologene: 28537
Bmp8a
Name: bone morphogenetic protein 8a
Synonyms: osteogenic protein 2, OP2, Bmp7r1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 12163
Homologene: 135983
Tmprss11d
Name: transmembrane protease, serine 11d
Synonyms: AsP
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231382
Homologene: 74519
Or4a73
Name: olfactory receptor family 4 subfamily A member 73
Synonyms: GA_x6K02T2Q125-51034790-51033846, MOR231-9, Olfr1246
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258788
Homologene: 74246
Rit2
Name: Ras-like without CAAX 2
Synonyms: Roc2, Rin
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 19762
Homologene: 2198
Prkar1b
Name: protein kinase, cAMP dependent regulatory, type I beta
Synonyms: RIbeta
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 19085
HGNC: HGNC:9390
Homologene: 37665
Ankrd55
Name: ankyrin repeat domain 55
Synonyms: C030011J08Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 77318
VEGA: 13
Homologene: 12674
Smo
Name: smoothened, frizzled class receptor
Synonyms: E130215L21Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 319757
Homologene: 4115
Cmtm3
Name: CKLF-like MARVEL transmembrane domain containing 3
Synonyms: 9430096L06Rik, BNAS2, Cklfsf3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 68119
Homologene: 11486
Coq3
Name: coenzyme Q3 methyltransferase
Synonyms: C77934
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230027
Homologene: 9683
Fv1
Name: Friend virus susceptibility 1
Synonyms: Rv-1, Fv-1, Rv1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 14349
Kat2b-ps
Name: K(lysine) acetyltransferase 2B, pseudogene
Synonyms: P/CAF pseudogene, Pcaf-ps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100504388
Brip1os
Name: BRCA1 interacting protein C-terminal helicase 1, opposite strand
Synonyms: 4632419I22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 74038
Vps29
Name: VPS29 retromer complex component
Synonyms: PEP11, 2010015D08Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 56433
Homologene: 9433
Duxf3
Name: double homeobox family member 3
Synonyms: 4933403O03Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 74399
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 46,029,564 bp
  • G to T, chromosome 1 at 46,233,689 bp
  • C to T, chromosome 1 at 97,721,128 bp
  • G to A, chromosome 1 at 190,799,466 bp
  • A to G, chromosome 2 at 57,111,865 bp
  • T to A, chromosome 2 at 76,754,824 bp
  • T to A, chromosome 2 at 89,591,088 bp
  • A to G, chromosome 2 at 118,787,575 bp
  • T to C, chromosome 3 at 56,019,424 bp
  • A to T, chromosome 4 at 21,900,416 bp
  • C to T, chromosome 4 at 40,948,007 bp
  • A to T, chromosome 4 at 123,313,295 bp
  • A to T, chromosome 4 at 123,527,458 bp
  • G to T, chromosome 4 at 147,870,089 bp
  • T to C, chromosome 5 at 30,064,116 bp
  • T to C, chromosome 5 at 86,338,887 bp
  • T to C, chromosome 5 at 93,391,185 bp
  • T to A, chromosome 5 at 100,518,539 bp
  • C to T, chromosome 5 at 107,815,678 bp
  • C to T, chromosome 5 at 114,244,551 bp
  • C to A, chromosome 5 at 122,359,714 bp
  • T to C, chromosome 5 at 128,991,091 bp
  • C to T, chromosome 5 at 139,106,628 bp
  • A to T, chromosome 6 at 29,754,467 bp
  • A to T, chromosome 6 at 113,759,238 bp
  • C to A, chromosome 7 at 30,190,126 bp
  • T to A, chromosome 7 at 103,173,559 bp
  • T to A, chromosome 8 at 67,908,861 bp
  • T to A, chromosome 8 at 70,539,789 bp
  • T to C, chromosome 8 at 104,343,833 bp
  • A to T, chromosome 9 at 19,846,616 bp
  • C to A, chromosome 9 at 21,027,886 bp
  • A to T, chromosome 9 at 62,060,305 bp
  • C to A, chromosome 10 at 58,231,093 bp
  • T to C, chromosome 10 at 109,880,654 bp
  • T to C, chromosome 11 at 49,113,052 bp
  • A to G, chromosome 11 at 58,141,586 bp
  • G to T, chromosome 11 at 69,516,036 bp
  • A to T, chromosome 11 at 84,215,873 bp
  • A to G, chromosome 11 at 86,285,146 bp
  • T to A, chromosome 11 at 102,436,244 bp
  • A to G, chromosome 11 at 110,115,538 bp
  • A to C, chromosome 12 at 72,579,364 bp
  • A to T, chromosome 12 at 112,908,922 bp
  • T to C, chromosome 13 at 17,803,930 bp
  • T to A, chromosome 13 at 70,753,196 bp
  • T to G, chromosome 13 at 112,336,226 bp
  • G to T, chromosome 14 at 97,969,959 bp
  • G to T, chromosome 17 at 35,470,124 bp
  • C to T, chromosome 17 at 56,435,716 bp
  • A to G, chromosome 18 at 5,049,339 bp
  • A to G, chromosome 18 at 31,316,852 bp
  • A to G, chromosome 18 at 37,982,035 bp
  • A to G, chromosome 18 at 61,309,063 bp
  • T to A, chromosome 19 at 6,251,338 bp
  • T to C, chromosome 19 at 53,541,234 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5350 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042929-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.