Strain Name:
C57BL/6J-MtgxR5420Btlr/Mmmh
Stock Number:
042988-MU
Citation ID:
RRID:MMRRC_042988-MU
Other Names:
R5420 (G1), C57BL/6J-MtgxR5420Btlr
Major Collection:

Gene Information

Trp53
Name: transformation related protein 53
Synonyms: p53, p44
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 22059
Homologene: 460
Nes
Name: nestin
Synonyms: Marc2, RC2, ESTM46, Ifaprc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 18008
HGNC: HGNC:7756
Homologene: 136487
Sash1
Name: SAM and SH3 domain containing 1
Synonyms: 2500002E12Rik, A330076K04Rik, 1100001C18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 70097
Homologene: 69182
Cacybp
Name: calcyclin binding protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12301
Homologene: 7649
Stam2
Name: signal transducing adaptor molecule (SH3 domain and ITAM motif) 2
Synonyms: 5730456G07Rik, 1200004O12Rik, Hbp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 56324
Homologene: 68490
Arhgap21
Name: Rho GTPase activating protein 21
Synonyms: ARHGAP10, 5530401C11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 71435
Homologene: 10822
Fmn2
Name: formin 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 54418
Homologene: 137334
Papola
Name: poly (A) polymerase alpha
Synonyms: PapIII, Plap
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 18789
Homologene: 23389
Dip2b
Name: disco interacting protein 2 homolog B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239667
Homologene: 72227
Rufy3
Name: RUN and FYVE domain containing 3
Synonyms: Rpipx, 6330416M07Rik, D5Bwg0860e, 2810428M05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 52822
Homologene: 87809
Kmt2a
Name: lysine (K)-specific methyltransferase 2A
Synonyms: Cxxc7, All1, Mll1, ALL-1, HTRX1, trithorax Drosophila, Mll
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 214162
HGNC: HGNC:7132
Homologene: 4338
Herc2
Name: HECT and RLD domain containing E3 ubiquitin protein ligase 2
Synonyms: D7H15F37S1, rjs, D7H15F32S1, D15F32S1h, jdf2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 15204
HGNC: HGNC:4868
Homologene: 3430
Rarb
Name: retinoic acid receptor, beta
Synonyms: RAR beta 2, Hap, RARbeta2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 218772
VEGA: 14
HGNC: HGNC:9865
Homologene: 68100
Clec16a
Name: C-type lectin domain family 16, member A
Synonyms: 4932416N17Rik, curt
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 74374
Homologene: 71019
Bdp1
Name: B double prime 1, subunit of RNA polymerase III transcription initiation factor IIIB
Synonyms: Tfnr, TAF3B1, TFIIIB90, TFIIIB150, TFC5, B130055N23Rik, G630013P12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 544971
Homologene: 34582
Thbs1
Name: thrombospondin 1
Synonyms: TSP1, Thbs-1, tbsp1, TSP-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 21825
Homologene: 31142
Crebbp
Name: CREB binding protein
Synonyms: KAT3A, CBP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 12914
HGNC: HGNC:2348
Homologene: 68393
Rasa3
Name: RAS p21 protein activator 3
Synonyms: R-Ras gap, hlb381, GAPIII activator 3, GAPIII, Ras GTPase-activating protein III, scat
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 19414
Homologene: 7217
Glt8d2
Name: glycosyltransferase 8 domain containing 2
Synonyms: 1110021D20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 74782
Homologene: 41826
Alas1
Name: aminolevulinic acid synthase 1
Synonyms: ALAS-N, 5-aminolevulinate synthase, Alas-1, succinyl-CoA: glycine C-succinyl transferase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 11655
HGNC: HGNC:396
Homologene: 55478
Ccdc47
Name: coiled-coil domain containing 47
Synonyms: asp4, 2610204L23Rik, calumin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 67163
Homologene: 41351
Akap2
Name: A kinase (PRKA) anchor protein 2
Synonyms: B230340M18Rik, AKAP-KL
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Ttn
Name: titin
Synonyms: D830007G01Rik, connectin, 1100001C23Rik, 2310036G12Rik, mdm, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru, L56
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Pcdh7
Name: protocadherin 7
Synonyms: BH-protocadherin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 54216
HGNC: HGNC:8659
Homologene: 36101
Eps8l1
Name: EPS8-like 1
Synonyms: EPS8R1, 2310051G19Rik, 4632407K17Rik, DRC3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 67425
Homologene: 15767
Ppfia2
Name: protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2
Synonyms: Liprin-alpha2, E130120L08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 327814
VEGA: 10
HGNC: HGNC:9246
Homologene: 27953
Arhgef25
Name: Rho guanine nucleotide exchange factor (GEF) 25
Synonyms: D10Ertd610e, 2410008H17Rik, GEFT
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 52666
VEGA: 10
Homologene: 32691
Scara5
Name: scavenger receptor class A, member 5
Synonyms: 4932433F15Rik, 4933425F03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 71145
Homologene: 12556
Ecm2
Name: extracellular matrix protein 2, female organ and adipocyte specific
Synonyms: tenonectin, 9030618O22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 407800
VEGA: 13
HGNC: HGNC:3154
Homologene: 1064
Capn3
Name: calpain 3
Synonyms: Capa-3, Lp82, Capa3, p94
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12335
HGNC: HGNC:1480
Homologene: 52
Eps8l3
Name: EPS8-like 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 99662
Homologene: 15878
Ifi206
Name: interferon activated gene 206
Synonyms: Gm4955, Pyblhin-C
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 102639543
HGNC: HGNC:5395
Homologene: 115929
Pappa
Name: pregnancy-associated plasma protein A
Synonyms: 8430414N03Rik, IGFBP-4ase, PAG1, PAPP-A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 18491
HGNC: HGNC:8602
Homologene: 31097
Plbd2
Name: phospholipase B domain containing 2
Synonyms: 66.3 kDa, 1300012G16Rik, p76
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 71772
Homologene: 41528
Mto1
Name: mitochondrial tRNA translation optimization 1
Synonyms: 5730419A02Rik, 2310039H01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 68291
Homologene: 5876
Pla2r1
Name: phospholipase A2 receptor 1
Synonyms: Pla2g1br, PLA2-I receptor, M-type receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18779
HGNC: HGNC:9042
Homologene: 32016
Mbl1
Name: mannose-binding lectin (protein A) 1
Synonyms: MBP-A, MBL-A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 17194
VEGA: 14
HGNC: HGNC:6921
Homologene: 55449
Zfp521
Name: zinc finger protein 521
Synonyms: Evi3, B930086A16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225207
VEGA: 18
Homologene: 9151
Adamts1
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 1
Synonyms: METH1, ADAM-TS1, ADAMTS-1, METH-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 11504
HGNC: HGNC:217
Homologene: 21381
Jade2
Name: jade family PHD finger 2
Synonyms: Phf15, 1200017K05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 76901
Homologene: 9082
Mmp1b
Name: matrix metallopeptidase 1b (interstitial collagenase)
Synonyms: Mcol-B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 83996
HGNC: HGNC:7155
Homologene: 130791
Actn3
Name: actinin alpha 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 11474
HGNC: HGNC:165
Homologene: 862
Rab7b
Name: RAB7B, member RAS oncogene family
Synonyms: 5430435G22Rik, Rab7b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226421
Homologene: 64833
Bpifa6
Name: BPI fold containing family A, member 6
Synonyms: Gm5840
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 545477
Homologene: 129594
Mrpl23
Name: mitochondrial ribosomal protein L23
Synonyms: Rpl23l, L23mrp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 19935
Homologene: 7922
Cyp2c23
Name: cytochrome P450, family 2, subfamily c, polypeptide 23
Synonyms: Cyp2c44
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 226143
Homologene: 115563
Rpain
Name: RPA interacting protein
Synonyms: 2400006N03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 69723
Homologene: 13006
Adgrg6
Name: adhesion G protein-coupled receptor G6
Synonyms: Gpr126, LOC215798, 1190004A11Rik, DREG
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 215798
Homologene: 10724
Thoc2l
Name: THO complex subunit 2-like
Synonyms: BC005561, Gm3179
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100042165
Cyp3a13
Name: cytochrome P450, family 3, subfamily a, polypeptide 13
Synonyms: IIIAm2, steroid inducible
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 13113
HGNC: HGNC:2640
Homologene: 133564
Edil3
Name: EGF-like repeats and discoidin I-like domains 3
Synonyms: Del-1, developmental endothelial locus-1, Del1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 13612
HGNC: HGNC:3173
Homologene: 21166
Fam184a
Name: family with sequence similarity 184, member A
Synonyms: 4930438C08Rik, 3110012E06Rik, 4930589M24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 75906
Homologene: 11600
Zfp677
Name: zinc finger protein 677
Synonyms: A830058L05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 210503
Homologene: 138402
Olfr866
Name: olfactory receptor 866
Synonyms: MOR145-5, GA_x6K02T2PVTD-13768406-13767468
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258551
Homologene: 133687
Rdh16f2
Name: RDH16 family member 2
Synonyms: BC089597
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216454
Homologene: 129514
Vmn1r223
Name: vomeronasal 1 receptor 223
Synonyms: Gm11330
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 100036518
Homologene: 110880
Cideb
Name: cell death-inducing DNA fragmentation factor, alpha subunit-like effector B
Synonyms: 1110030C18Rik, CIDE-B, DFFA-like B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 12684
HGNC: HGNC:1977
Homologene: 7666
Scrn1
Name: secernin 1
Synonyms: 2810019K23Rik, 6330535A03Rik, SES1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 69938
Homologene: 8853
Pick1
Name: protein interacting with C kinase 1
Synonyms: Prkcabp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 18693
HGNC: HGNC:9394
Homologene: 7470
Lipi
Name: lipase, member I
Synonyms: D930038D03Rik, lpd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 320355
Homologene: 77864
Nfkbie
Name: nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, epsilon
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 18037
VEGA: 17
HGNC: HGNC:7799
Homologene: 36160
D030055H07Rik
Name: RIKEN cDNA D030055H07 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 654798
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 131,698,426 bp
  • T to C, chromosome 1 at 160,208,344 bp
  • T to A, chromosome 1 at 173,481,033 bp
  • A to T, chromosome 1 at 174,698,778 bp
  • G to T, chromosome 2 at 20,881,086 bp
  • G to A, chromosome 2 at 52,736,293 bp
  • G to T, chromosome 2 at 60,495,256 bp
  • A to T, chromosome 2 at 76,811,243 bp
  • G to T, chromosome 2 at 118,113,155 bp
  • T to C, chromosome 2 at 120,495,296 bp
  • T to A, chromosome 2 at 153,989,330 bp
  • A to T, chromosome 3 at 87,977,002 bp
  • A to T, chromosome 3 at 107,883,985 bp
  • G to A, chromosome 4 at 57,856,062 bp
  • T to A, chromosome 4 at 57,856,434 bp
  • T to G, chromosome 4 at 65,335,780 bp
  • T to A, chromosome 5 at 57,720,187 bp
  • T to C, chromosome 5 at 88,640,659 bp
  • T to A, chromosome 5 at 104,518,359 bp
  • A to G, chromosome 5 at 120,494,482 bp
  • T to G, chromosome 5 at 137,898,981 bp
  • T to A, chromosome 6 at 54,512,063 bp
  • T to G, chromosome 7 at 4,470,161 bp
  • A to T, chromosome 7 at 56,203,830 bp
  • A to G, chromosome 7 at 142,536,137 bp
  • C to T, chromosome 8 at 13,576,365 bp
  • T to C, chromosome 9 at 7,384,897 bp
  • T to C, chromosome 9 at 20,027,059 bp
  • A to T, chromosome 9 at 44,848,336 bp
  • T to A, chromosome 9 at 78,452,827 bp
  • G to T, chromosome 9 at 106,157,707 bp
  • G to T, chromosome 9 at 106,234,159 bp
  • G to A, chromosome 10 at 8,746,186 bp
  • A to T, chromosome 10 at 14,426,986 bp
  • A to G, chromosome 10 at 53,633,657 bp
  • T to C, chromosome 10 at 82,652,682 bp
  • A to T, chromosome 10 at 106,835,701 bp
  • A to T, chromosome 10 at 127,187,274 bp
  • C to T, chromosome 10 at 127,877,074 bp
  • T to C, chromosome 11 at 51,818,607 bp
  • T to C, chromosome 11 at 69,588,320 bp
  • A to T, chromosome 11 at 70,977,690 bp
  • C to T, chromosome 11 at 106,210,350 bp
  • A to G, chromosome 12 at 105,806,495 bp
  • A to G, chromosome 13 at 23,249,505 bp
  • A to T, chromosome 13 at 49,527,734 bp
  • T to C, chromosome 13 at 89,131,772 bp
  • T to C, chromosome 13 at 100,066,043 bp
  • T to A, chromosome 14 at 16,434,249 bp
  • C to T, chromosome 14 at 41,157,196 bp
  • A to C, chromosome 14 at 55,758,291 bp
  • CG to C, chromosome 14 at 65,759,662 bp
  • A to G, chromosome 15 at 79,248,840 bp
  • C to T, chromosome 15 at 100,205,173 bp
  • C to T, chromosome 16 at 4,107,458 bp
  • G to A, chromosome 16 at 10,731,679 bp
  • A to G, chromosome 16 at 75,555,869 bp
  • A to T, chromosome 16 at 85,799,609 bp
  • T to C, chromosome 17 at 21,397,913 bp
  • T to A, chromosome 17 at 45,560,206 bp
  • A to T, chromosome 18 at 13,844,087 bp
  • TGCGCAGC to T, chromosome 19 at 4,865,344 bp
  • A to C, chromosome 19 at 44,015,664 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5420 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042988-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.