Strain Name:
C57BL/6J-MtgxR5424Btlr/Mmmh
Stock Number:
042990-MU
Citation ID:
RRID:MMRRC_042990-MU
Other Names:
R5424 (G1), C57BL/6J-MtgxR5424Btlr
Major Collection:

Gene Information

Epha2
Name: Eph receptor A2
Synonyms: Sek-2, Myk2, Eck, Sek2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 13836
HGNC: HGNC:3386
Homologene: 20929
Nes
Name: nestin
Synonyms: RC2, Ifaprc2, ESTM46, Marc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 18008
HGNC: HGNC:7756
Homologene: 136487
Mtmr7
Name: myotubularin related protein 7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 54384
HGNC: HGNC:7454
Homologene: 99732
Ddx54
Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 54
Synonyms: 2410015A15Rik, APR-5, DP97
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 71990
Homologene: 5590
Senp7
Name: SUMO1/sentrin specific peptidase 7
Synonyms: 2900036C23Rik, 6030449K19Rik, 2410152H17Rik, 2810413I22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 66315
Homologene: 10778
Actb
Name: actin, beta
Synonyms: E430023M04Rik, Actx, A-X actin-like protein, beta-actin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 11461
HGNC: HGNC:132
Homologene: 110648
Rnf123
Name: ring finger protein 123
Synonyms: KPC1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 84585
Homologene: 11112
Gas8
Name: growth arrest specific 8
Synonyms: Gas11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 104346
HGNC: HGNC:4166
Homologene: 1135
Zfp622
Name: zinc finger protein 622
Synonyms: D15Ertd806e, 1110033B05Rik, ZPR9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 52521
VEGA: 15
Homologene: 74377
Cdc45
Name: cell division cycle 45
Synonyms: Cdc45l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 12544
HGNC: HGNC:1739
Homologene: 2616
Mgat4a
Name: mannoside acetylglucosaminyltransferase 4, isoenzyme A
Synonyms: 9530018I07Rik, GnT-IVa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 269181
HGNC: HGNC:7047
Homologene: 8153
Clca2
Name: chloride channel accessory 2
Synonyms: Clca5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 229933
HGNC: HGNC:2016
Homologene: 4765
Ermard
Name: ER membrane associated RNA degradation
Synonyms: 2410011O22Rik, 2210404J11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 381062
Homologene: 19936
Fchsd1
Name: FCH and double SH3 domains 1
Synonyms: A030002D08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 319262
Homologene: 14127
Aire
Name: autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 11634
HGNC: HGNC:360
Homologene: 327
Nelfa
Name: negative elongation factor complex member A, Whsc2
Synonyms: Nelf-A, Whsc2, Whsc2h
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 24116
Homologene: 68478
Kdelr2
Name: KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 2
Synonyms: 1110007A14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 66913
HGNC: HGNC:6305
Homologene: 129146
Gfra2
Name: glial cell line derived neurotrophic factor family receptor alpha 2
Synonyms: GFR alpha-2, GFR alpha 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 14586
VEGA: 14
HGNC: HGNC:4244
Homologene: 1145
Marveld2
Name: MARVEL (membrane-associating) domain containing 2
Synonyms: Tric-c, Tric-a, Tricellulin, Tric-b, Tric, Mrvldc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 218518
VEGA: 13
Homologene: 27037
Lama2
Name: laminin, alpha 2
Synonyms: mer, merosin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 16773
HGNC: HGNC:6482
Homologene: 37306
Dnah14
Name: dynein, axonemal, heavy chain 14
Synonyms: Gm980, A230079K17Rik, Dnahc14, LOC381311
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 240960
HGNC: HGNC:2945
Homologene: 90078
Zfp646
Name: zinc finger protein 646
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 233905
Homologene: 8802
Wdr17
Name: WD repeat domain 17
Synonyms: 3010002I12Rik, B230207L18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 244484
Homologene: 12460
Ndrg2
Name: N-myc downstream regulated gene 2
Synonyms: Ndr2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 29811
VEGA: 14
Homologene: 22785
Cpne8
Name: copine VIII
Synonyms: 1200003E11Rik, 1500031E20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 66871
Homologene: 12049
Ifit2
Name: interferon-induced protein with tetratricopeptide repeats 2
Synonyms: Ifi54
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 15958
HGNC: HGNC:5409
Homologene: 1187
Lrp1b
Name: low density lipoprotein-related protein 1B
Synonyms: 9630004P12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 94217
HGNC: HGNC:6693
Homologene: 56810
Tnn
Name: tenascin N
Synonyms: Tnw, tenascin-W
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 329278
Homologene: 18634
Mroh2b
Name: maestro heat-like repeat family member 2B
Synonyms: 4930455B06Rik, Heatr7b2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 223825
VEGA: 15
Homologene: 128807
Scn5a
Name: sodium channel, voltage-gated, type V, alpha
Synonyms: SkM2, Nav1.5c, mH1, Nav1.5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 20271
Homologene: 22738
Ada
Name: adenosine deaminase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 11486
HGNC: HGNC:186
Homologene: 37249
Tgm7
Name: transglutaminase 7
Synonyms: TGz
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 640543
Homologene: 14192
Whrn
Name: whirlin
Synonyms: Dfnb31, Ush2d, C430046P22Rik, wi, 1110035G07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 73750
Homologene: 18739
Lipg
Name: lipase, endothelial
Synonyms: 3110013K01Rik, endothelial lipase, EL, mEDL
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 16891
VEGA: 18
HGNC: HGNC:6623
Homologene: 21218
Abca14
Name: ATP-binding cassette, sub-family A (ABC1), member 14
Synonyms: 1700110B15Rik, 4930539G24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 67928
Homologene: 86128
Slc51a
Name: solute carrier family 51, alpha subunit
Synonyms: D630035O19Rik, OSTalpha, Osta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 106407
Homologene: 44941
Rpl22l1
Name: ribosomal protein L22 like 1
Synonyms: 3110001N18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 68028
Homologene: 134718
Adam22
Name: a disintegrin and metallopeptidase domain 22
Synonyms: 2900022I03Rik, MDC2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 11496
HGNC: HGNC:201
Homologene: 37898
Fam186a
Name: family with sequence similarity 186, member A
Synonyms: 1700030F18Rik, LOC380973
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 72277
Atp8a1
Name: ATPase, aminophospholipid transporter (APLT), class I, type 8A, member 1
Synonyms: Atp3a2, B230107D19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 11980
Homologene: 48402
Tbxas1
Name: thromboxane A synthase 1, platelet
Synonyms: CYP5A1, CYP5, TXAS, TXS
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 21391
Homologene: 130979
Obox5
Name: oocyte specific homeobox 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 252829
Homologene: 44937
Olfr689
Name: olfactory receptor 689
Synonyms: GA_x6K02T2PBJ9-7942985-7943947, MOR40-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 258745
Homologene: 128074
AA986860
Name: expressed sequence AA986860
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 212439
Homologene: 49741
Plscr4
Name: phospholipid scramblase 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 235527
Homologene: 23217
Tcl1b5
Name: T cell leukemia/lymphoma 1B, 5
Synonyms: D12Ertd644e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 27382
VEGA: 12
Homologene: 83274
Olfr1058
Name: olfactory receptor 1058
Synonyms: MOR190-2, GA_x6K02T2Q125-47855818-47854868
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258386
Homologene: 74093
Gm2128
Name: predicted gene 2128
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 100039269
Homologene: 45597
Lrrc8b
Name: leucine rich repeat containing 8 family, member B
Synonyms: 2210408K08Rik, R75581
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 433926
Homologene: 9103
Nipa1
Name: non imprinted in Prader-Willi/Angelman syndrome 1 homolog (human)
Synonyms: 1110027G09Rik, A830014A18Rik, Spg6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 233280
Homologene: 42327
Srcin1
Name: SRC kinase signaling inhibitor 1
Synonyms: p140Cap, P140
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 56013
Homologene: 10324
Pla1a
Name: phospholipase A1 member A
Synonyms: Ps-pla1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 85031
VEGA: 16
Homologene: 9294
Gm10770
Name: predicted gene 10770
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 544945
VEGA: 2
Ighg2b
Name: immunoglobulin heavy constant gamma 2B
Synonyms: IgG2b, gamma2b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 16016
Impdh2-ps
Name: inosine monophosphate dehydrogenase 2, pseudogene
Synonyms: Gm15210, ENSMUSG00000071041
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 100042069
HGNC: HGNC:6053
Homologene: 48919
Pcdhga7
Name: protocadherin gamma subfamily A, 7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 93715
HGNC: HGNC:8705
Homologene: 36377
Slc2a6
Name: solute carrier family 2 (facilitated glucose transporter), member 6
Synonyms: F630103L12Rik, Glut6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 227659
Homologene: 69236
Gm2617
Name: predicted gene 2617
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 37,466,555 bp
  • C to A, chromosome 1 at 130,742,941 bp
  • T to A, chromosome 1 at 160,122,702 bp
  • T to G, chromosome 1 at 181,763,310 bp
  • G to A, chromosome 2 at 27,025,317 bp
  • G to A, chromosome 2 at 41,498,337 bp
  • G to A, chromosome 2 at 86,385,840 bp
  • T to A, chromosome 2 at 121,099,041 bp
  • T to C, chromosome 2 at 150,179,028 bp
  • T to A, chromosome 2 at 163,728,125 bp
  • T to C, chromosome 3 at 28,806,898 bp
  • G to T, chromosome 3 at 87,978,824 bp
  • A to G, chromosome 3 at 145,084,181 bp
  • T to C, chromosome 4 at 63,418,452 bp
  • T to A, chromosome 4 at 141,318,940 bp
  • C to A, chromosome 5 at 8,090,182 bp
  • C to T, chromosome 5 at 33,921,845 bp
  • T to C, chromosome 5 at 67,812,100 bp
  • T to C, chromosome 5 at 105,480,703 bp
  • T to C, chromosome 5 at 120,619,861 bp
  • A to G, chromosome 5 at 142,905,551 bp
  • A to C, chromosome 5 at 143,418,144 bp
  • A to G, chromosome 6 at 39,027,905 bp
  • T to A, chromosome 7 at 15,758,882 bp
  • C to T, chromosome 7 at 41,155,593 bp
  • C to A, chromosome 7 at 55,979,475 bp
  • T to C, chromosome 7 at 105,314,571 bp
  • T to C, chromosome 7 at 120,211,554 bp
  • C to A, chromosome 7 at 127,882,703 bp
  • A to G, chromosome 8 at 40,606,830 bp
  • C to T, chromosome 8 at 54,681,399 bp
  • G to T, chromosome 8 at 100,031,509 bp
  • C to A, chromosome 8 at 123,526,512 bp
  • T to A, chromosome 9 at 92,490,022 bp
  • T to A, chromosome 9 at 108,052,005 bp
  • T to C, chromosome 9 at 119,501,734 bp
  • G to A, chromosome 10 at 26,984,396 bp
  • A to T, chromosome 10 at 78,036,719 bp
  • T to A, chromosome 11 at 97,537,059 bp
  • T to C, chromosome 12 at 105,180,016 bp
  • T to C, chromosome 12 at 113,307,930 bp
  • T to C, chromosome 13 at 100,612,187 bp
  • A to G, chromosome 14 at 51,908,885 bp
  • A to T, chromosome 14 at 70,895,847 bp
  • A to T, chromosome 15 at 4,941,612 bp
  • T to C, chromosome 15 at 25,984,769 bp
  • T to A, chromosome 15 at 90,516,057 bp
  • G to T, chromosome 15 at 99,945,763 bp
  • C to T, chromosome 16 at 18,795,897 bp
  • C to A, chromosome 16 at 32,478,747 bp
  • T to A, chromosome 16 at 38,414,775 bp
  • A to G, chromosome 16 at 56,186,108 bp
  • T to A, chromosome 17 at 15,059,770 bp
  • G to A, chromosome 18 at 37,715,335 bp
  • C to T, chromosome 18 at 37,959,873 bp
  • T to A, chromosome 18 at 74,954,253 bp
  • T to C, chromosome 19 at 9,323,956 bp
  • T to A, chromosome 19 at 34,574,058 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5424 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042990-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.