Strain Name:
C57BL/6J-MtgxR5446Btlr/Mmmh
Stock Number:
043011-MU
Citation ID:
RRID:MMRRC_043011-MU
Other Names:
R5446 (G1), C57BL/6J-MtgxR5446Btlr
Major Collection:

Gene Information

Cadm1
Name: cell adhesion molecule 1
Synonyms: RA175C, 2900073G06Rik, Igsf4, Igsf4a, RA175A, Necl2, SynCam, Tslc1, 3100001I08Rik, RA175N, SgIGSF, RA175B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 54725
HGNC: HGNC:5951
Homologene: 8641
Stam2
Name: signal transducing adaptor molecule (SH3 domain and ITAM motif) 2
Synonyms: 1200004O12Rik, 5730456G07Rik, Hbp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 56324
Homologene: 68490
Med13l
Name: mediator complex subunit 13-like
Synonyms: Trap240L, 6330591G05Rik, 2210413I17Rik, 9030618F05Rik, Thrap2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 76199
Homologene: 25256
Helz
Name: helicase with zinc finger domain
Synonyms: 3110078M01Rik, 9630002H22Rik, 9430093I07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 78455
Homologene: 8918
Sft2d1
Name: SFT2 domain containing 1
Synonyms: 5630401J11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 106489
Homologene: 34525
M6pr
Name: mannose-6-phosphate receptor, cation dependent
Synonyms: CD-MPR, Mpr46
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 17113
HGNC: HGNC:6752
Homologene: 31086
Npnt
Name: nephronectin
Synonyms: POEM, 1110009H02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 114249
Homologene: 14138
Themis
Name: thymocyte selection associated
Synonyms: Tsepa, E430004N04Rik, Gasp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 210757
Homologene: 72287
Rreb1
Name: ras responsive element binding protein 1
Synonyms: B930013M22Rik, 1110037N09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 68750
Homologene: 2218
Zfp451
Name: zinc finger protein 451
Synonyms: 4930515K21Rik, 4933435G09Rik, Kiaa0576-hp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 98403
Homologene: 9188
Klhl23
Name: kelch-like 23
Synonyms: C130068N17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 277396
Homologene: 18628
Mmp27
Name: matrix metallopeptidase 27
Synonyms: LOC234911
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 234911
Homologene: 23345
Dync2h1
Name: dynein cytoplasmic 2 heavy chain 1
Synonyms: m407Asp, DHC1b, b2b414Clo, Dnchc2, 4432416O06Rik, D030010H02Rik, D330044F14Rik, m152Asp, DHC2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 110350
HGNC: HGNC:2962
Homologene: 14468
Zfp746
Name: zinc finger protein 746
Synonyms: 2810407L07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 69228
Homologene: 19783
Adar
Name: adenosine deaminase, RNA-specific
Synonyms: Adar1p110, ADAR1, Adar1p150, mZaADAR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 56417
HGNC: HGNC:225
Homologene: 9281
Cfap74
Name: cilia and flagella associated protein 74
Synonyms: 2010015L04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 544678
Homologene: 129584
Ttn
Name: titin
Synonyms: D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, mdm, 1100001C23Rik, shru, connectin, L56
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 22138
Homologene: 130650
Bcat2
Name: branched chain aminotransferase 2, mitochondrial
Synonyms: Eca40, Bcat-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 12036
HGNC: HGNC:977
Homologene: 81684
Ankrd52
Name: ankyrin repeat domain 52
Synonyms: G431002C21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 237615
Homologene: 18227
Gm5134
Name: predicted gene 5134
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 333669
Homologene: 129891
Lipm
Name: lipase, family member M
Synonyms: Lipl3, 4632427C23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 78753
VEGA: 19
Homologene: 28267
Mroh2a
Name: maestro heat-like repeat family member 2A
Synonyms: ENSMUSG00000044873, OTTMUSG00000020804, Heatr7b1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 100040766
Homologene: 85300
Synm
Name: synemin, intermediate filament protein
Synonyms: Dmn, 4930412K21Rik, Synemin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 233335
Homologene: 9081
Pwwp2b
Name: PWWP domain containing 2B
Synonyms: D930023J19Rik, Pwwp2, D7Ertd517e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 101631
Homologene: 19056
Prkcg
Name: protein kinase C, gamma
Synonyms: PKCgamma, Pkcc, Prkcc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 18752
HGNC: HGNC:9402
Homologene: 20602
BC051142
Name: cDNA sequence BC051142
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 407788
Homologene: 128168
Ak9
Name: adenylate kinase 9
Synonyms: Akd2, Gm7127, LOC215946, Akd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 633979
Homologene: 67934
Pnpla8
Name: patatin-like phospholipase domain containing 8
Synonyms: iPLA2 gamma, 1200006O19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 67452
Homologene: 12136
Gm5155
Name: predicted gene 5155
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 381852
Homologene: 115938
Serpinb10
Name: serine (or cysteine) peptidase inhibitor, clade B (ovalbumin), member 10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 241197
HGNC: HGNC:8942
Homologene: 68430
Cdh2
Name: cadherin 2
Synonyms: Ncad, N-CAD, N-cadherin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 12558
HGNC: HGNC:1759
Homologene: 20424
Jade2
Name: jade family PHD finger 2
Synonyms: 1200017K05Rik, Phf15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 76901
Homologene: 9082
St6galnac1
Name: ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 1
Synonyms: ST6GalNAc I, Siat7a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 20445
Homologene: 7937
Vmn2r90
Name: vomeronasal 2, receptor 90
Synonyms: EG626942
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 626942
Arhgap15
Name: Rho GTPase activating protein 15
Synonyms: 5830480G12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 76117
Homologene: 41271
Bscl2
Name: Berardinelli-Seip congenital lipodystrophy 2 (seipin)
Synonyms: Gng3lg, seipin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 14705
Homologene: 32032
Vmn2r112
Name: vomeronasal 2, receptor 112
Synonyms: EG628185
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 628185
Homologene: 86604
Gm9573
Name: predicted gene 9573
Synonyms: Muc21, epiglycanin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 672682
Plekhd1
Name: pleckstrin homology domain containing, family D (with coiled-coil domains) member 1
Synonyms: 3830431G21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 217682
Homologene: 15090
Edil3
Name: EGF-like repeats and discoidin I-like domains 3
Synonyms: Del1, developmental endothelial locus-1, Del-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 13612
HGNC: HGNC:3173
Homologene: 21166
Krt75
Name: keratin 75
Synonyms: Krt2-6hf, K6hf, 4732468K03Rik, Krtcap1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 109052
Homologene: 20983
Olfr1257
Name: olfactory receptor 1257
Synonyms: GA_x6K02T2Q125-51319458-51320387, MOR232-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258984
Homologene: 115501
Mrps9
Name: mitochondrial ribosomal protein S9
Synonyms: 2310002A08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 69527
Homologene: 32565
Paip2b
Name: poly(A) binding protein interacting protein 2B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 232164
Homologene: 25798
Cnbd2
Name: cyclic nucleotide binding domain containing 2
Synonyms: 5430421B09Rik, 4921517L17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 70873
Homologene: 15440
Crb2
Name: crumbs family member 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 241324
Homologene: 45474
Vpreb1
Name: pre-B lymphocyte gene 1
Synonyms: CD179a, Vpreb-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 22362
Homologene: 84741
Zfand2b
Name: zinc finger, AN1 type domain 2B
Synonyms: 1110060O18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 68818
Homologene: 32671
Smok3c
Name: sperm motility kinase 3C
Synonyms: EG622486
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 622486
Homologene: 128738
Pcdhac2
Name: protocadherin alpha subfamily C, 2
Synonyms: CNRc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 353237
HGNC: HGNC:8677
Homologene: 49562
Gm5250
Name: predicted gene 5250
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 383490
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 13,062,169 bp
  • A to G, chromosome 1 at 33,777,528 bp
  • T to A, chromosome 1 at 42,905,463 bp
  • T to C, chromosome 1 at 75,170,480 bp
  • A to T, chromosome 1 at 88,254,965 bp
  • T to C, chromosome 1 at 107,546,922 bp
  • T to C, chromosome 2 at 37,795,449 bp
  • T to A, chromosome 2 at 43,828,760 bp
  • G to A, chromosome 2 at 52,736,293 bp
  • T to A, chromosome 2 at 69,824,238 bp
  • A to T, chromosome 2 at 76,811,243 bp
  • A to T, chromosome 2 at 89,881,549 bp
  • A to C, chromosome 2 at 156,367,661 bp
  • A to G, chromosome 3 at 89,740,179 bp
  • A to G, chromosome 3 at 132,908,369 bp
  • T to A, chromosome 4 at 155,447,609 bp
  • A to G, chromosome 5 at 118,742,397 bp
  • A to C, chromosome 5 at 138,064,633 bp
  • T to C, chromosome 6 at 48,064,173 bp
  • T to C, chromosome 6 at 83,814,862 bp
  • G to T, chromosome 6 at 122,312,384 bp
  • A to T, chromosome 7 at 3,330,264 bp
  • A to T, chromosome 7 at 17,917,465 bp
  • G to A, chromosome 7 at 45,585,145 bp
  • T to C, chromosome 7 at 67,735,974 bp
  • T to A, chromosome 7 at 139,255,150 bp
  • T to C, chromosome 9 at 7,144,217 bp
  • T to A, chromosome 9 at 7,573,515 bp
  • T to C, chromosome 9 at 47,818,847 bp
  • A to G, chromosome 10 at 28,761,345 bp
  • A to G, chromosome 10 at 41,420,509 bp
  • T to C, chromosome 10 at 75,995,836 bp
  • G to T, chromosome 10 at 128,388,561 bp
  • A to G, chromosome 11 at 51,816,959 bp
  • T to C, chromosome 11 at 107,632,204 bp
  • T to A, chromosome 11 at 116,766,269 bp
  • A to G, chromosome 12 at 44,290,585 bp
  • A to G, chromosome 12 at 80,720,636 bp
  • G to A, chromosome 13 at 37,898,497 bp
  • A to C, chromosome 13 at 89,184,838 bp
  • T to C, chromosome 15 at 101,571,067 bp
  • A to T, chromosome 16 at 16,868,690 bp
  • G to T, chromosome 17 at 8,326,961 bp
  • A to C, chromosome 17 at 17,712,202 bp
  • A to G, chromosome 17 at 22,618,250 bp
  • T to C, chromosome 17 at 34,440,893 bp
  • T to A, chromosome 17 at 35,622,503 bp
  • T to C, chromosome 18 at 16,646,627 bp
  • T to A, chromosome 18 at 37,145,200 bp
  • A to G, chromosome 19 at 8,846,200 bp
  • C to T, chromosome 19 at 34,117,887 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5446 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
043011-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.