Strain Name:
C57BL/6J-MtgxR5448Btlr/Mmmh
Stock Number:
043013-MU
Citation ID:
RRID:MMRRC_043013-MU
Other Names:
R5448 (G1), C57BL/6J-MtgxR5448Btlr
Major Collection:

Strain Information

Prrx1
Name: paired related homeobox 1
Synonyms: K-2, Prx1, mHox, Pmx1, mHox, A230024N07Rik, MHox1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18933
HGNC: HGNC:9142
Homologene: 7896
L3mbtl2
Name: L3MBTL2 polycomb repressive complex 1 subunit
Synonyms: 4732493N06Rik, m4mbt
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 214669
Homologene: 12882
Prpf40a
Name: pre-mRNA processing factor 40A
Synonyms: FBP11, 2810012K09Rik, Fnbp3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 56194
Homologene: 6377
Fbxo38
Name: F-box protein 38
Synonyms: SP329, 6030410I24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 107035
VEGA: 18
Homologene: 34526
Pgr
Name: progesterone receptor
Synonyms: NR3C3, PR, 9930019P03Rik, PR-B, PR-A, ENSMUSG00000074510
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 18667
HGNC: HGNC:8910
Homologene: 713
Scn10a
Name: sodium channel, voltage-gated, type X, alpha
Synonyms: Nav1.8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 20264
VEGA: 9
Homologene: 21300
Shld2
Name: shieldin complex subunit 2
Synonyms: 3110001K24Rik, Fam35a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 75698
VEGA: 14
Homologene: 56840
Klhl2
Name: kelch-like 2, Mayven
Synonyms: ABP-KELCH, 6030411N21Rik, Mav, 8530402H02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 77113
HGNC: HGNC:6353
Homologene: 21416
Wdtc1
Name: WD and tetratricopeptide repeats 1
Synonyms: LOC230796, adp, adipose
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230796
Homologene: 8997
Itgae
Name: integrin alpha E, epithelial-associated
Synonyms: CD103, alpha-E1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 16407
HGNC: HGNC:6147
Homologene: 113560
Maml2
Name: mastermind like transcriptional coactivator 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 270118
Homologene: 134147
Or1e35
Name: olfactory receptor family 1 subfamily E member 35
Synonyms: GA_x6K02T2P1NL-4062605-4061667, MOR135-10, Olfr395
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 259007
Ccdc88b
Name: coiled-coil domain containing 88B
Synonyms: 2610041P08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 78317
VEGA: 19
Homologene: 49992
Ccdc180
Name: coiled-coil domain containing 180
Synonyms: LOC381522, E230008N13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 381522
Homologene: 117988
Tshz3
Name: teashirt zinc finger family member 3
Synonyms: A630038G13Rik, Zfp537, teashirt3, Tsh3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 243931
Homologene: 10835
Plce1
Name: phospholipase C, epsilon 1
Synonyms: 4933403A21Rik, PLCepsilon
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 74055
Homologene: 9478
Rfx6
Name: regulatory factor X, 6
Synonyms: 4930572O07Rik, Rfxdc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 320995
Homologene: 18318
C1galt1
Name: core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase, 1
Synonyms: core 1 beta3-Gal-T, 2210410E06Rik, T-synthase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 94192
Homologene: 10599
Slc24a1
Name: solute carrier family 24 (sodium/potassium/calcium exchanger), member 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 214111
VEGA: 9
Homologene: 3472
Pcnx2
Name: pecanex homolog 2
Synonyms: E330039K12Rik, Pcnxl2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 270109
HGNC: HGNC:8736
Homologene: 8987
Pde6c
Name: phosphodiesterase 6C, cGMP specific, cone, alpha prime
Synonyms: cpfl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 110855
VEGA: 19
HGNC: HGNC:8787
Homologene: 4521
Actn3
Name: actinin alpha 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 11474
HGNC: HGNC:165
Homologene: 862
Or4c112
Name: olfactory receptor family 4 subfamily C member 112
Synonyms: GA_x6K02T2Q125-50504545-50503631, MOR233-4, Olfr1217
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258903
Homologene: 73985
Cdadc1
Name: cytidine and dCMP deaminase domain containing 1
Synonyms: NYD-SP15, 2310010M10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 71891
VEGA: 14
Homologene: 12794
Tmem8b
Name: transmembrane protein 8B
Synonyms: 4930500O05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242409
Homologene: 72894
Clip2
Name: CAP-GLY domain containing linker protein 2
Synonyms: CLIP-115, WSCR4, Cyln2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 269713
HGNC: HGNC:2586
Homologene: 20718
Trappc1
Name: trafficking protein particle complex 1
Synonyms: MUM2, BET5, D11Ertd172e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 245828
Homologene: 41425
4930505A04Rik
Name: RIKEN cDNA 4930505A04 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 75087
Homologene: 18988
Krt1
Name: keratin 1
Synonyms: Krt2-1, Krt-2.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 16678
VEGA: 15
HGNC: HGNC:6412
Homologene: 38146
Slfn3
Name: schlafen 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 20557
Homologene: 133236
Ubash3b
Name: ubiquitin associated and SH3 domain containing, B
Synonyms: 2810457I06Rik, TULA-2, Sts-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 72828
Homologene: 13152
Zdhhc22
Name: zinc finger, DHHC-type containing 22
Synonyms: LOC238331
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 238331
VEGA: 12
Homologene: 45486
Ppl
Name: periplakin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 19041
VEGA: 16
HGNC: HGNC:9273
Homologene: 2026
Rdh16f2
Name: RDH16 family member 2
Synonyms: BC089597
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216454
Homologene: 129514
Dnase1l1
Name: deoxyribonuclease 1-like 1
Synonyms: G4.8, Dnl1ll, 2310005K03Rik, Dnase1ll
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 69537
HGNC: HGNC:2957
Homologene: 4896
Gm9887
Name: predicted gene 9887
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 163,248,298 bp
  • G to T, chromosome 2 at 53,156,926 bp
  • T to G, chromosome 2 at 89,023,501 bp
  • G to A, chromosome 4 at 43,673,992 bp
  • A to T, chromosome 4 at 45,920,913 bp
  • G to A, chromosome 4 at 133,294,297 bp
  • T to C, chromosome 5 at 134,514,048 bp
  • C to T, chromosome 6 at 7,866,658 bp
  • A to G, chromosome 7 at 36,771,229 bp
  • C to A, chromosome 8 at 64,822,990 bp
  • T to C, chromosome 8 at 125,888,149 bp
  • A to G, chromosome 9 at 8,922,637 bp
  • A to C, chromosome 9 at 13,706,467 bp
  • A to G, chromosome 9 at 41,037,435 bp
  • A to G, chromosome 9 at 64,948,327 bp
  • A to T, chromosome 9 at 119,687,947 bp
  • T to C, chromosome 10 at 51,683,637 bp
  • C to T, chromosome 10 at 127,877,063 bp
  • C to T, chromosome 11 at 30,426,349 bp
  • T to C, chromosome 11 at 69,325,535 bp
  • T to C, chromosome 11 at 73,133,908 bp
  • T to C, chromosome 11 at 73,906,611 bp
  • T to A, chromosome 11 at 83,214,605 bp
  • C to A, chromosome 12 at 69,372,091 bp
  • T to C, chromosome 12 at 86,988,567 bp
  • G to A, chromosome 14 at 34,268,370 bp
  • T to A, chromosome 14 at 59,573,826 bp
  • T to C, chromosome 15 at 81,684,333 bp
  • T to A, chromosome 15 at 101,849,029 bp
  • C to T, chromosome 16 at 5,107,566 bp
  • A to T, chromosome 18 at 62,522,457 bp
  • T to C, chromosome 19 at 4,863,211 bp
  • T to A, chromosome 19 at 6,854,580 bp
  • A to G, chromosome 19 at 38,133,175 bp
  • T to C, chromosome 19 at 38,779,917 bp
  • C to T, chromosome X at 74,277,038 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5448 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043013-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.