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Strain Name:
C57BL/6J-MtgxR5484Btlr/Mmmh
Stock Number:
043045-MU
Citation ID:
RRID:MMRRC_043045-MU
Other Names:
R5484 (G1), C57BL/6J-MtgxR5484Btlr
Major Collection:

Strain Information

Tgfb1
Name: transforming growth factor, beta 1
Synonyms: TGF-beta 1, Tgfb-1, Tgfb, TGFbeta1, TGF-beta1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 21803
Homologene: 540
Chd7
Name: chromodomain helicase DNA binding protein 7
Synonyms: GENA 60, GENA 47, Gena 52, Cyn, A730019I05Rik, WBE1, Whi, Todo, Obt, Mt, Lda, Flo, Edy, Dz, Cycn
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 320790
Homologene: 19067
Sh3gl2
Name: SH3-domain GRB2-like 2
Synonyms: EEN-B1, endophilin I, Sh3d2a, 9530001L19Rik, B930049H17Rik, endophilin A1, EEN1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 20404
Homologene: 20652
Mga
Name: MAX gene associated
Synonyms: Mga, Mad5, C130042M01Rik, D030062C11Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 29808
Homologene: 49351
Cyrib
Name: CYFIP related Rac1 interactor B
Synonyms: 0910001A06Rik, Fam49b
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223601
VEGA: 15
Homologene: 9599
Zfp341
Name: zinc finger protein 341
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228807
Homologene: 13127
Tcf7l2
Name: transcription factor 7 like 2, T cell specific, HMG box
Synonyms: TCF4E, TCF4B, mTcf-4E, mTcf-4B, Tcf-4, Tcf4
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 21416
Homologene: 7564
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 78,476,196 bp
  • A to G, chromosome 1 at 88,258,664 bp
  • T to C, chromosome 1 at 150,426,888 bp
  • T to C, chromosome 1 at 150,675,540 bp
  • A to G, chromosome 1 at 151,718,086 bp
  • T to A, chromosome 1 at 173,641,074 bp
  • C to T, chromosome 2 at 31,393,054 bp
  • T to G, chromosome 2 at 119,916,626 bp
  • T to C, chromosome 2 at 151,471,931 bp
  • T to C, chromosome 2 at 154,625,001 bp
  • T to C, chromosome 2 at 164,597,729 bp
  • A to T, chromosome 3 at 95,337,258 bp
  • G to C, chromosome 4 at 8,828,258 bp
  • A to G, chromosome 4 at 83,693,962 bp
  • T to C, chromosome 4 at 85,398,923 bp
  • T to C, chromosome 4 at 98,774,785 bp
  • A to T, chromosome 4 at 114,845,051 bp
  • T to A, chromosome 5 at 26,117,932 bp
  • T to C, chromosome 5 at 72,358,484 bp
  • C to G, chromosome 5 at 113,614,929 bp
  • T to C, chromosome 5 at 125,787,733 bp
  • C to A, chromosome 5 at 142,100,186 bp
  • T to C, chromosome 5 at 143,928,125 bp
  • T to C, chromosome 5 at 145,243,635 bp
  • T to A, chromosome 6 at 18,427,690 bp
  • C to A, chromosome 6 at 29,917,155 bp
  • T to G, chromosome 6 at 38,389,122 bp
  • T to A, chromosome 6 at 48,834,533 bp
  • G to T, chromosome 6 at 73,092,116 bp
  • C to A, chromosome 6 at 85,868,012 bp
  • T to C, chromosome 7 at 8,380,124 bp
  • A to C, chromosome 7 at 25,688,149 bp
  • G to A, chromosome 7 at 30,720,672 bp
  • T to C, chromosome 7 at 41,649,853 bp
  • A to T, chromosome 7 at 44,999,958 bp
  • A to G, chromosome 9 at 58,290,782 bp
  • C to G, chromosome 9 at 86,545,288 bp
  • A to T, chromosome 9 at 106,501,908 bp
  • A to T, chromosome 9 at 107,946,146 bp
  • A to G, chromosome 10 at 52,358,846 bp
  • GC to G, chromosome 10 at 59,892,752 bp
  • A to G, chromosome 10 at 79,742,054 bp
  • T to C, chromosome 10 at 85,146,724 bp
  • T to C, chromosome 10 at 85,928,537 bp
  • T to A, chromosome 10 at 103,025,316 bp
  • G to A, chromosome 11 at 58,923,825 bp
  • A to G, chromosome 11 at 67,251,818 bp
  • G to A, chromosome 11 at 78,404,822 bp
  • A to G, chromosome 11 at 85,914,404 bp
  • T to C, chromosome 11 at 95,387,805 bp
  • A to T, chromosome 11 at 110,184,073 bp
  • G to A, chromosome 12 at 76,578,400 bp
  • T to C, chromosome 12 at 101,575,916 bp
  • A to G, chromosome 13 at 38,184,038 bp
  • A to G, chromosome 13 at 55,826,353 bp
  • G to T, chromosome 13 at 56,363,174 bp
  • C to T, chromosome 13 at 67,481,254 bp
  • A to T, chromosome 14 at 69,963,542 bp
  • A to G, chromosome 15 at 4,908,981 bp
  • T to A, chromosome 15 at 63,943,207 bp
  • T to C, chromosome 15 at 85,931,282 bp
  • T to C, chromosome 15 at 89,164,209 bp
  • A to G, chromosome 16 at 23,429,275 bp
  • T to C, chromosome 17 at 56,104,932 bp
  • T to C, chromosome 17 at 56,575,346 bp
  • T to A, chromosome 18 at 36,998,103 bp
  • T to A, chromosome 18 at 49,889,464 bp
  • A to G, chromosome 19 at 10,207,325 bp
  • G to A, chromosome 19 at 12,035,828 bp
  • T to C, chromosome 19 at 42,147,833 bp
  • C to A, chromosome 19 at 55,919,508 bp
  • C to T, chromosome 19 at 56,479,828 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5484 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043045-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.