Strain Name:
C57BL/6J-MtgxR5660Btlr/Mmmh
Stock Number:
043173-MU
Citation ID:
RRID:MMRRC_043173-MU
Other Names:
R5660 (G1), C57BL/6J-MtgxR5660Btlr
Major Collection:

Strain Information

Matr3
Name: matrin 3
Synonyms: D030046F20Rik, 2810017I02Rik, 1110061A14Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 17184
HGNC: HGNC:6912
Homologene: 7830
Sema6d
Name: sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D
Synonyms: 1110067B02Rik, Sema6D-6, Sema6D-5, Sema6D-4, Sema6D-2, Sema6D-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 214968
Homologene: 16195
Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1-n, BPAG1, Bpag1, Bpag, ah, bullous pemphigoid antigen 1, athetoid, Macf2, 2310001O04Rik, nmf203, A830042E19Rik, nmf339
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Abcc8
Name: ATP-binding cassette, sub-family C member 8
Synonyms: SUR1, Sur, D930031B21Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20927
HGNC: HGNC:59
Homologene: 68048
Helb
Name: helicase (DNA) B
Synonyms: D10Ertd664e
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 117599
Homologene: 50463
Cad
Name: carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
Synonyms: 2410008J01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 69719
HGNC: HGNC:1424
Homologene: 1412
Smg1
Name: SMG1 nonsense mediated mRNA decay associated PI3K related kinase
Synonyms: C130002K18Rik, 5430435M13Rik, 2610207I05Rik, SMG1 homolog, phosphatidylinositol 3-kinase-related kinase (C. elegans)
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233789
Homologene: 56697
Rps6ka5
Name: ribosomal protein S6 kinase, polypeptide 5
Synonyms: 6330404E13Rik, MSK1, 3110005L17Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 73086
VEGA: 12
Homologene: 48302
Atg2b
Name: autophagy related 2B
Synonyms: C630028L02Rik, C030004M05Rik, 2410024A21Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 76559
VEGA: 12
Homologene: 9974
Uvssa
Name: UV stimulated scaffold protein A
Synonyms: D330017J19Rik, 4933407H18Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 71101
Homologene: 13807
Cftr
Name: cystic fibrosis transmembrane conductance regulator
Synonyms: Abcc7
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12638
HGNC: HGNC:1884
Homologene: 55465
Galnt4
Name: polypeptide N-acetylgalactosaminyltransferase 4
Synonyms: ppGaNTase-T4
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 14426
Homologene: 74511
Msh6
Name: mutS homolog 6
Synonyms: Msh6, GTBP, Gtmbp
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 17688
VEGA: 17
HGNC: HGNC:7329
Homologene: 149
Abcf1
Name: ATP-binding cassette, sub-family F member 1
Synonyms: Abc50, D17Wsu166e, GCN20
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224742
HGNC: HGNC:70
Homologene: 849
Flt3
Name: FMS-like tyrosine kinase 3
Synonyms: CD135, Flk-2, Flt-3, Flk2, wmfl
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14255
HGNC: HGNC:3765
Homologene: 3040
Adar
Name: adenosine deaminase, RNA-specific
Synonyms: ADAR1, mZaADAR, Adar1p150, Adar1p110
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 56417
HGNC: HGNC:225
Homologene: 9281
Adamts13
Name: ADAM metallopeptidase with thrombospondin type 1 motif 13
Synonyms: LOC279028, vWF-CP mRNA for von Willebrand factor-cleaving
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 279028
HGNC: HGNC:1366
Homologene: 16372
Akap3
Name: A kinase anchor protein 3
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 11642
HGNC: HGNC:373
Homologene: 4688
Fat2
Name: FAT atypical cadherin 2
Synonyms: LOC245827, mKIAA0811, Fath2, EMI2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 245827
HGNC: HGNC:3596
Homologene: 1110
Zfyve9
Name: zinc finger, FYVE domain containing 9
Synonyms: Madhip
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230597
HGNC: HGNC:6775
Homologene: 3527
Gm5422
Name: predicted pseudogene 5422
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 432448
VEGA: 10
Slc5a8
Name: solute carrier family 5 (iodide transporter), member 8
Synonyms: SMCT
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216225
VEGA: 10
Homologene: 64832
Tpo
Name: thyroid peroxidase
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 22018
Homologene: 461
Dph3b-ps
Name: diphthamide biosynthesis 3, pseudogene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 620949
Mmp23
Name: matrix metallopeptidase 23
Synonyms: cysteine array matrix metalloproteinase, CA-MMP
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 26561
HGNC: HGNC:7171
Homologene: 3424
Adamts2
Name: ADAM metallopeptidase with thrombospondin type 1 motif 2
Synonyms: ADAM-TS2, hPCPNI, a disintegrin and metalloproteinase with thrombospondin repeats, procollagen N-proteinase
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216725
HGNC: HGNC:218
Homologene: 8597
Ccdc81
Name: coiled-coil domain containing 81
Synonyms: 4921513D09Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 70884
Homologene: 128447
Rora
Name: RAR-related orphan receptor alpha
Synonyms: Nr1f1, 9530021D13Rik, tmgc26
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 19883
Homologene: 56594
Col6a4
Name: collagen, type VI, alpha 4
Synonyms: EG235580, 1110001D15Rik, Dvwa, Vwa6
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 68553
Homologene: 130754
Or8g4
Name: olfactory receptor family 8 subfamily G member 4
Synonyms: GA_x6K02T2PVTD-33447884-33448816, MOR171-30P, Olfr967
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258086
VEGA: 9
HGNC: HGNC:8484
Homologene: 115535
Themis2
Name: thymocyte selection associated family member 2
Synonyms: ICB-1, BC013712
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230787
Homologene: 21009
Ptar1
Name: protein prenyltransferase alpha subunit repeat containing 1
Synonyms: 2010013L17Rik, 1700084D09Rik, 4930428J16Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 72351
VEGA: 19
Homologene: 34254
Igfn1
Name: immunoglobulin-like and fibronectin type III domain containing 1
Synonyms: 9830123M21Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226438
Homologene: 130054
Simc1
Name: SUMO-interacting motifs containing 1
Synonyms: 4732471D19Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 319719
Homologene: 131217
Ano5
Name: anoctamin 5
Synonyms: Gdd1, Tmem16e
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233246
Homologene: 100071
Fam83c
Name: family with sequence similarity 83, member C
Synonyms: 5530400B04Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 71405
Homologene: 18669
Cbs
Name: cystathionine beta-synthase
Synonyms: HIP4
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 12411
HGNC: HGNC:1550
Homologene: 37258
Eif2b4
Name: eukaryotic translation initiation factor 2B, subunit 4 delta
Synonyms: Eif2b
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 13667
HGNC: HGNC:3260
Homologene: 5976
Arhgap11a
Name: Rho GTPase activating protein 11A
Synonyms: GAP (1-12), 6530401L14Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228482
Homologene: 8862
Zfp493
Name: zinc finger protein 493
Synonyms: 2900054J07Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 72958
Ido1
Name: indoleamine 2,3-dioxygenase 1
Synonyms: Ido, Indo
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 15930
HGNC: HGNC:6059
Homologene: 48082
Sgsh
Name: N-sulfoglucosamine sulfohydrolase (sulfamidase)
Synonyms: sulphamidase, 4632406A19Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 27029
Homologene: 167
Smyd2
Name: SET and MYND domain containing 2
Synonyms: Zmynd14, 1110020E07Rik, KMT3C
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226830
Homologene: 32466
Akr1c6
Name: aldo-keto reductase family 1, member C6
Synonyms: estradiol 17-beta-dehydrogenase (A-specific), 3alpha-HSD, Akr1c1, Hsd17b5
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 83702
Homologene: 84695
Or12d13
Name: olfactory receptor family 12 subfamily D member 13
Synonyms: GA_x6K02T2PSCP-1798423-1797482, MOR250-3, MOR250-8_p, Olfr103
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 258830
Homologene: 115555
Wnt2
Name: wingless-type MMTV integration site family, member 2
Synonyms: Wnt-2, Irp, Int1l1, m-irp, Mirp, 2610510E18Rik, Wnt2a
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 22413
Homologene: 20719
4932416K20Rik
Name: RIKEN cDNA 4932416K20 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 442809
Homologene: 133775
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 34,282,493 bp
  • A to G, chromosome 1 at 135,970,414 bp
  • G to A, chromosome 1 at 189,885,382 bp
  • T to C, chromosome 2 at 26,996,749 bp
  • T to A, chromosome 2 at 113,841,910 bp
  • T to C, chromosome 2 at 124,658,760 bp
  • G to A, chromosome 2 at 155,829,589 bp
  • T to A, chromosome 2 at 177,086,431 bp
  • T to C, chromosome 3 at 89,735,594 bp
  • A to G, chromosome 4 at 43,547,732 bp
  • T to C, chromosome 4 at 108,719,168 bp
  • T to C, chromosome 4 at 132,796,256 bp
  • C to T, chromosome 4 at 155,651,253 bp
  • T to A, chromosome 5 at 31,076,847 bp
  • A to T, chromosome 5 at 31,191,156 bp
  • T to A, chromosome 5 at 33,387,800 bp
  • T to C, chromosome 5 at 147,369,481 bp
  • T to A, chromosome 6 at 18,028,146 bp
  • T to A, chromosome 6 at 18,313,687 bp
  • C to T, chromosome 6 at 126,865,291 bp
  • T to C, chromosome 7 at 46,108,404 bp
  • G to A, chromosome 7 at 51,583,814 bp
  • T to C, chromosome 7 at 89,893,129 bp
  • C to T, chromosome 7 at 118,143,347 bp
  • T to C, chromosome 8 at 24,591,542 bp
  • A to T, chromosome 8 at 104,797,334 bp
  • G to A, chromosome 9 at 39,750,767 bp
  • A to G, chromosome 9 at 68,653,921 bp
  • A to G, chromosome 9 at 105,996,116 bp
  • A to T, chromosome 10 at 31,250,052 bp
  • C to T, chromosome 10 at 88,919,428 bp
  • A to G, chromosome 10 at 99,109,535 bp
  • G to A, chromosome 10 at 120,111,079 bp
  • A to T, chromosome 11 at 50,776,645 bp
  • T to A, chromosome 11 at 55,284,176 bp
  • A to G, chromosome 11 at 119,350,981 bp
  • T to A, chromosome 12 at 30,100,496 bp
  • G to T, chromosome 12 at 100,619,580 bp
  • A to T, chromosome 12 at 105,649,124 bp
  • G to A, chromosome 13 at 4,449,054 bp
  • A to G, chromosome 13 at 54,547,089 bp
  • A to G, chromosome 13 at 67,787,086 bp
  • T to G, chromosome 13 at 106,547,032 bp
  • A to T, chromosome 17 at 31,624,246 bp
  • A to T, chromosome 17 at 35,963,647 bp
  • A to G, chromosome 17 at 37,336,644 bp
  • A to G, chromosome 17 at 87,984,719 bp
  • C to A, chromosome 18 at 35,572,094 bp
  • T to A, chromosome 19 at 23,694,412 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5660 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043173-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.