Strain Name:
C57BL/6J-MtgxR5852Btlr/Mmmh
Stock Number:
043227-MU
Citation ID:
RRID:MMRRC_043227-MU
Other Names:
R5852 (G1), C57BL/6J-MtgxR5852Btlr
Major Collection:

Gene Information

Sema3e
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E
Synonyms: Semah
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 20349
Homologene: 8247
Pnoc
Name: prepronociceptin
Synonyms: N23K, OFQ/N, proorphanin, N/OFQ, Npnc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 18155
VEGA: 14
HGNC: HGNC:9163
Homologene: 4537
Tti1
Name: TELO2 interacting protein 1
Synonyms: 2610036D13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 75425
Homologene: 40969
Sephs1
Name: selenophosphate synthetase 1
Synonyms: 1110046B24Rik, SPS1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 109079
Homologene: 56558
Wdr1
Name: WD repeat domain 1
Synonyms: D5Wsu185e, rede, Aip1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 22388
Homologene: 6628
Zfp106
Name: zinc finger protein 106
Synonyms: Cd-1, sirm, Sh3bp3, H3a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 20402
Homologene: 40787
Iqgap2
Name: IQ motif containing GTPase activating protein 2
Synonyms: 4933417J23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 544963
VEGA: 13
HGNC: HGNC:6111
Homologene: 101543
Lrrk2
Name: leucine-rich repeat kinase 2
Synonyms: cI-46, 4921513O20Rik, D630001M17Rik, 9330188B09Rik, LOC381026
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 66725
Homologene: 18982
Klhl23
Name: kelch-like 23
Synonyms: C130068N17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 277396
Homologene: 18628
Mia3
Name: melanoma inhibitory activity 3
Synonyms: LOC385255, 9130229H14Rik, Tango, A930039G15Rik, B230399H06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 338366
Homologene: 85165
Dync2h1
Name: dynein cytoplasmic 2 heavy chain 1
Synonyms: 4432416O06Rik, DHC2, b2b414Clo, Dnchc2, m407Asp, m152Asp, D030010H02Rik, D330044F14Rik, DHC1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 110350
HGNC: HGNC:2962
Homologene: 14468
Cbwd1
Name: COBW domain containing 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 226043
VEGA: 19
Homologene: 6843
Anpep
Name: alanyl (membrane) aminopeptidase
Synonyms: Cd13, Apn, aminopeptidase N
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 16790
HGNC: HGNC:500
Homologene: 68163
Dnhd1
Name: dynein heavy chain domain 1
Synonyms: 8030491N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 77505
Homologene: 131117
Pkhd1
Name: polycystic kidney and hepatic disease 1
Synonyms: FPC, tigmin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 241035
HGNC: HGNC:9016
Homologene: 16336
Cntn3
Name: contactin 3
Synonyms: Pang
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 18488
HGNC: HGNC:2173
Homologene: 7461
Ncoa6
Name: nuclear receptor coactivator 6
Synonyms: AIB3, RAP250, PRIP, NRC, ASC2, ASC-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 56406
Homologene: 137337
Irak3
Name: interleukin-1 receptor-associated kinase 3
Synonyms: 4833428C18Rik, IRAK-M
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 73914
Homologene: 36215
Abcb5
Name: ATP-binding cassette, sub-family B (MDR/TAP), member 5
Synonyms: 9230106F14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 77706
HGNC: HGNC:46
Homologene: 83488
Gucy1a2
Name: guanylate cyclase 1, soluble, alpha 2
Synonyms: A230060L24Rik, 6330407I18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 234889
HGNC: HGNC:4684
Homologene: 47953
Pappa2
Name: pappalysin 2
Synonyms: PLAC3, placenta-specific 3, pregnancy-associated plasma preproprotein-A2, pregnancy-associated plasma protein-E, Pappe, PAPP-A2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 23850
Homologene: 10661
Plxnb1
Name: plexin B1
Synonyms: 2900002G15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 235611
HGNC: HGNC:9103
Homologene: 130508
Apcdd1
Name: adenomatosis polyposis coli down-regulated 1
Synonyms: EIG180, Drapc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 494504
VEGA: 18
Homologene: 77420
Hs6st3
Name: heparan sulfate 6-O-sulfotransferase 3
Synonyms: 6OST3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 50787
VEGA: 14
Homologene: 89201
Ccdc154
Name: coiled-coil domain containing 154
Synonyms: ntl, LOC207209
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 207209
Homologene: 19361
Phyhip
Name: phytanoyl-CoA hydroxylase interacting protein
Synonyms: PAHX-AP1, C630010D02Rik, PAHX-AP#1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 105653
Homologene: 8847
Nox4
Name: NADPH oxidase 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 50490
HGNC: HGNC:7891
Homologene: 41065
Cntn6
Name: contactin 6
Synonyms: NB-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 53870
HGNC: HGNC:2176
Homologene: 8702
Lancl1
Name: LanC (bacterial lantibiotic synthetase component C)-like 1
Synonyms: Gpr69a, p40, LanC-like protein 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 14768
HGNC: HGNC:6508
Homologene: 4417
Fam189a1
Name: family with sequence similarity 189, member A1
Synonyms: 5730507A09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 70638
Homologene: 77705
Prss29
Name: protease, serine 29
Synonyms: mISP-2, Isp2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 114662
VEGA: 17
Homologene: 70877
Il27
Name: interleukin 27
Synonyms: IL-27p28, p28, IL-27, Il30
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 246779
Homologene: 17087
Crisp3
Name: cysteine-rich secretory protein 3
Synonyms: CRISP-3, Aeg2, SGP28, CRS3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 11572
Homologene: 135665
Atp6v1g2
Name: ATPase, H+ transporting, lysosomal V1 subunit G2
Synonyms: VAG2, lysosomal 13kDa, 1500002D01Rik, Atp6g2, NG38
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 66237
HGNC: HGNC:862
Homologene: 41518
Scrn3
Name: secernin 3
Synonyms: 4833415E20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 74616
Homologene: 11601
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 20,377,408 bp
  • A to G, chromosome 1 at 67,021,111 bp
  • T to C, chromosome 1 at 158,717,014 bp
  • A to G, chromosome 1 at 183,332,859 bp
  • A to G, chromosome 2 at 4,899,528 bp
  • T to A, chromosome 2 at 69,824,269 bp
  • T to C, chromosome 2 at 73,331,005 bp
  • A to G, chromosome 2 at 120,516,006 bp
  • G to T, chromosome 2 at 155,405,499 bp
  • A to G, chromosome 2 at 158,000,673 bp
  • A to G, chromosome 5 at 14,252,817 bp
  • A to G, chromosome 5 at 38,537,175 bp
  • A to T, chromosome 6 at 102,420,416 bp
  • G to T, chromosome 6 at 104,835,745 bp
  • T to A, chromosome 7 at 64,775,831 bp
  • C to T, chromosome 7 at 79,838,972 bp
  • C to T, chromosome 7 at 87,338,964 bp
  • T to A, chromosome 7 at 105,695,748 bp
  • T to A, chromosome 7 at 126,592,614 bp
  • T to C, chromosome 9 at 3,865,460 bp
  • A to T, chromosome 9 at 7,011,290 bp
  • A to G, chromosome 9 at 109,106,450 bp
  • G to A, chromosome 10 at 120,182,602 bp
  • T to A, chromosome 12 at 118,927,404 bp
  • C to T, chromosome 13 at 95,675,372 bp
  • C to T, chromosome 14 at 65,411,222 bp
  • T to C, chromosome 14 at 70,461,929 bp
  • G to T, chromosome 14 at 119,869,326 bp
  • G to A, chromosome 15 at 91,755,949 bp
  • T to C, chromosome 17 at 25,163,209 bp
  • A to G, chromosome 17 at 25,322,434 bp
  • T to C, chromosome 17 at 35,237,573 bp
  • A to T, chromosome 17 at 40,225,820 bp
  • T to C, chromosome 18 at 62,937,063 bp
  • A to G, chromosome 19 at 24,955,405 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5852 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
043227-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.