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Strain Name:
C57BL/6J-MtgxR5191Btlr/Mmmh
Stock Number:
043263-MU
Citation ID:
RRID:MMRRC_043263-MU
Other Names:
R5191 (G1)
Major Collection:

Strain Information

Limch1
Name: LIM and calponin homology domains 1
Synonyms: 3732412D22Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 77569
Homologene: 18953
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Arfgef2
Name: ARF guanine nucleotide exchange factor 2
Synonyms: BIG2, E230011G24Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 99371
Homologene: 111880
Usp30
Name: ubiquitin specific peptidase 30
Synonyms: 6330590F17Rik, D5Ertd483e
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100756
Homologene: 32789
Etl4
Name: enhancer trap locus 4
Synonyms: Etl-4, E330027G05Rik, 6620402G01Rik, 9430077C05Rik, Skt, Sickle tail
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 208618
Homologene: 10477
Crh
Name: corticotropin releasing hormone
Synonyms: CRF, corticotropin releasing factor, LOC383938, corticotropin-releasing factor
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 12918
HGNC: HGNC:2355
Homologene: 599
Marf1
Name: meiosis regulator and mRNA stability 1
Synonyms: 4921513D23Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 223989
VEGA: 16
Homologene: 40967
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 40,743,893 bp
  • A to T, chromosome 1 at 72,274,977 bp
  • T to C, chromosome 1 at 74,147,663 bp
  • C to T, chromosome 1 at 134,994,010 bp
  • G to T, chromosome 2 at 20,339,999 bp
  • T to G, chromosome 2 at 37,084,978 bp
  • T to C, chromosome 2 at 45,002,600 bp
  • T to C, chromosome 2 at 66,093,706 bp
  • C to T, chromosome 2 at 76,879,088 bp
  • A to T, chromosome 2 at 130,737,403 bp
  • C to T, chromosome 2 at 158,201,035 bp
  • G to A, chromosome 2 at 164,826,019 bp
  • A to G, chromosome 2 at 166,876,511 bp
  • C to T, chromosome 2 at 168,662,063 bp
  • A to T, chromosome 3 at 8,545,575 bp
  • T to C, chromosome 3 at 19,693,929 bp
  • T to C, chromosome 3 at 108,751,990 bp
  • G to A, chromosome 3 at 108,752,551 bp
  • A to T, chromosome 4 at 66,841,379 bp
  • G to T, chromosome 4 at 104,995,797 bp
  • A to G, chromosome 4 at 123,472,962 bp
  • T to C, chromosome 5 at 67,027,561 bp
  • T to C, chromosome 5 at 100,405,511 bp
  • G to A, chromosome 5 at 104,486,681 bp
  • T to A, chromosome 5 at 110,184,307 bp
  • C to T, chromosome 5 at 114,065,694 bp
  • T to A, chromosome 6 at 43,115,866 bp
  • A to G, chromosome 7 at 7,116,023 bp
  • T to G, chromosome 7 at 16,875,450 bp
  • A to G, chromosome 7 at 23,809,022 bp
  • C to A, chromosome 7 at 44,550,416 bp
  • A to T, chromosome 7 at 90,375,652 bp
  • A to G, chromosome 7 at 141,858,539 bp
  • T to C, chromosome 8 at 43,519,991 bp
  • A to G, chromosome 8 at 85,084,459 bp
  • G to A, chromosome 9 at 19,713,334 bp
  • G to T, chromosome 9 at 21,107,497 bp
  • G to A, chromosome 9 at 107,911,551 bp
  • C to A, chromosome 10 at 7,867,818 bp
  • T to C, chromosome 10 at 39,970,672 bp
  • C to T, chromosome 11 at 6,515,105 bp
  • A to T, chromosome 11 at 46,756,197 bp
  • A to T, chromosome 11 at 55,052,893 bp
  • G to A, chromosome 11 at 78,497,119 bp
  • T to A, chromosome 11 at 97,338,149 bp
  • C to T, chromosome 11 at 111,072,471 bp
  • T to C, chromosome 12 at 16,580,828 bp
  • C to A, chromosome 12 at 76,612,834 bp
  • A to T, chromosome 14 at 52,077,507 bp
  • T to A, chromosome 14 at 56,061,009 bp
  • A to G, chromosome 14 at 76,111,989 bp
  • C to T, chromosome 14 at 118,911,393 bp
  • A to G, chromosome 15 at 11,327,757 bp
  • T to C, chromosome 15 at 76,175,165 bp
  • A to G, chromosome 15 at 101,137,065 bp
  • A to G, chromosome 16 at 14,146,078 bp
  • G to A, chromosome 16 at 32,794,476 bp
  • A to C, chromosome 17 at 28,974,370 bp
  • A to G, chromosome 17 at 30,746,765 bp
  • A to G, chromosome 18 at 69,642,883 bp
  • C to T, chromosome 18 at 77,780,229 bp
  • T to A, chromosome 18 at 82,621,423 bp
  • G to A, chromosome 19 at 8,344,393 bp
  • A to C, chromosome 19 at 39,802,591 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5191 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043263-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.