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Approximately half of Myo10tm1a homozygotes exhibit the lethal neural tube defect of exencephaly. The tm1a homozygotes that are not affected by exencephaly exhibit 100% incidence of a white belly-spot and 100% incidence of persistent hyaloid vasculature. Numerous other eye defects are observed at lower frequencies including anophthalmia, microphthalmia, coloboma, and defects in lens formation. Approximately half of tm1a homozygotes exhibit syndactyly. Based on KOMP data, tm2 homozygotes also have white belly spots and a high incidence of persistent hyaloid vasculature. Based on the characterization reported in our manuscript on Myo10 null mice, the tm1a and m1j alleles exhibit a similar core phenotype, but the tm1a allele has conditional potential, making it very useful given the high level of lethality in Myo10 nulls and the large number of defects in the nulls.
Homozygous: Born at less than half of the expected Mendelian ratio, which attributed largely to the high frequency (~50%) of exencephaly in the homozygotes. Homozygotes that are not exencephalic are able to develop into adult mice and breed. Homozygotes average ~20% lighter in weight than wild-type and 100% of homozygotes had a white belly spot. 100% of homozygotes whose eyes were dissected exhibited persistent fetal vasculature. Approximately 50% of Myo10tm1a homozygotes exhibited webbed digits.
Hetero/Hemizygous: Heterozygotes appear normal and are not obviously different from wild-type.
Heimsath EG Jr, Yim YI, Mustapha M, Hammer JA, Cheney RE. Myosin-X knockout issemi-lethal and demonstrates that myosin-X functions in neural tube closure,pigmentation, hyaloid vasculature regression, and filopodia formation. Sci Rep.2017 Dec 11;7(1):17354. doi: 10.1038/s41598-017-17638-x. (Medline PMID: 29229982)
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.