Register New MMRRC Account
Reset Password
Register for New Mouse Models
Click Here for Additional Contact Information
Availability & Fees Order this Strain
The CD45-minigene transgenic construct 'VGI-0020' (also shown as LFAiT200 in reference PMID:12393487) was injected into (BALB/c x C57BL/6)F2 oocytes. This line is "Strain B", which was 1 of 3 founders. The Strain B founder was mated to C57BL/6J mice (JAX Strain #002725) and progeny were screened for the presence of the transgene. The colony was then backcrossed to C57BL/6J mice for 10 generations. The colony was crossed with the CD45 knockout strain on the C57BL/6 background until the transgene locus was homozygous on the CD45 knockout background. Mice homozygous for the Strain B transgene locus on the CD45 knockout background were then sib-mated after the backcrossing was complete, for at least 25 generations to date.
Byth KF, Conroy LA, Howlett S, Smith AJ, May J, Alexander DR, Holmes N.CD45-null transgenic mice reveal a positive regulatory role for CD45 in earlythymocyte development, in the selection of CD4+CD8+ thymocytes, and B cellmaturation. J Exp Med. 1996 Apr 1;183(4):1707-18. (Medline PMID: 8666928)
Panchal RG, Ulrich RL, Bradfute SB, Lane D, Ruthel G, Kenny TA, Iversen PL,Anderson AO, Gussio R, Raschke WC, Bavari S. Reduced expression of CD45protein-tyrosine phosphatase provides protection against anthrax pathogenesis. J Biol Chem. 2009 May 8;284(19):12874-85. doi: 10.1074/jbc.M809633200. Epub 2009Mar 6. (Medline PMID: 19269962)
Panchal RG, Bradfute SB, Peyser BD, Warfield KL, Ruthel G, Lane D, Kenny TA,Anderson AO, Raschke WC, Bavari S. Reduced levels of protein tyrosine phosphataseCD45 protect mice from the lethal effects of Ebola virus infection. Cell HostMicrobe. 2009 Aug 20;6(2):162-73. doi: 10.1016/j.chom.2009.07.003. (Medline PMID: 19683682)
Virts EL, Raschke WC. The role of intron sequences in high level expressionfrom CD45 cDNA constructs. J Biol Chem. 2001 Jun 8;276(23):19913-20. Epub 2001Mar 16. (Medline PMID: 11389149)
Virts EL, Diago O, Raschke WC. A CD45 minigene restores regulated isoformexpression and immune function in CD45-deficient mice: therapeutic implicationsfor human CD45-null severe combined immunodeficiency. Blood. 2003 Feb1;101(3):849-55. Epub 2002 Sep 19. (Medline PMID: 12393487)
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.