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Strain Name:
C57BL/6J-MtgxR5838Btlrk/Mmmh
Stock Number:
044058-MU
Citation ID:
RRID:MMRRC_044058-MU
Other Names:
R5838 (G1)
Major Collection:

Strain Information

Pkd1
Name: polycystin 1, transient receptor potential channel interacting
Synonyms: polycystin-1, PC1, PC-1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18763
VEGA: 17
HGNC: HGNC:9008
Homologene: 250
Kif5a
Name: kinesin family member 5A
Synonyms: Kns, Kif5, D10Bwg0738e, Khc
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 16572
VEGA: 10
HGNC: HGNC:6323
Homologene: 55861
Setd5
Name: SET domain containing 5
Synonyms: 2900045N06Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 72895
Homologene: 12485
Kmt2c
Name: lysine (K)-specific methyltransferase 2C
Synonyms: E330008K23Rik, HALR, Mll3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231051
Homologene: 46480
Ndrg4
Name: N-myc downstream regulated gene 4
Synonyms: D8Bwg1337e, Ndr1-rs, SMAP-8, Ndr4
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234593
Homologene: 23228
Galnt10
Name: polypeptide N-acetylgalactosaminyltransferase 10
Synonyms: GalNAc-T10, Galnt9, C330012K04Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 171212
Homologene: 14924
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 58,412,660 bp
  • A to G, chromosome 1 at 90,144,945 bp
  • T to A, chromosome 1 at 106,347,132 bp
  • C to A, chromosome 1 at 138,534,363 bp
  • T to A, chromosome 1 at 173,732,387 bp
  • A to G, chromosome 1 at 183,423,571 bp
  • C to A, chromosome 1 at 191,070,113 bp
  • A to G, chromosome 2 at 13,580,190 bp
  • C to A, chromosome 2 at 31,457,807 bp
  • T to C, chromosome 2 at 150,585,857 bp
  • T to C, chromosome 2 at 156,051,993 bp
  • A to G, chromosome 3 at 35,985,082 bp
  • G to A, chromosome 3 at 108,624,736 bp
  • C to A, chromosome 4 at 21,759,622 bp
  • C to T, chromosome 4 at 32,754,547 bp
  • G to T, chromosome 4 at 63,225,528 bp
  • T to C, chromosome 4 at 123,452,154 bp
  • A to G, chromosome 4 at 143,896,920 bp
  • T to A, chromosome 5 at 21,899,113 bp
  • T to C, chromosome 5 at 25,284,471 bp
  • T to A, chromosome 5 at 30,366,513 bp
  • T to C, chromosome 5 at 36,940,187 bp
  • T to A, chromosome 5 at 61,809,225 bp
  • T to A, chromosome 5 at 73,012,030 bp
  • A to T, chromosome 6 at 42,361,646 bp
  • T to C, chromosome 6 at 91,271,848 bp
  • T to C, chromosome 6 at 113,119,435 bp
  • T to C, chromosome 6 at 135,225,267 bp
  • T to A, chromosome 7 at 5,097,166 bp
  • A to G, chromosome 7 at 104,596,897 bp
  • A to T, chromosome 7 at 108,894,085 bp
  • T to C, chromosome 7 at 109,808,444 bp
  • A to T, chromosome 7 at 125,867,655 bp
  • A to T, chromosome 8 at 47,512,559 bp
  • A to T, chromosome 8 at 95,706,793 bp
  • G to T, chromosome 9 at 21,795,393 bp
  • T to A, chromosome 9 at 42,415,872 bp
  • G to A, chromosome 9 at 43,005,608 bp
  • A to G, chromosome 9 at 88,391,776 bp
  • G to T, chromosome 9 at 106,895,488 bp
  • A to G, chromosome 9 at 108,978,143 bp
  • A to G, chromosome 10 at 37,136,167 bp
  • G to A, chromosome 10 at 59,223,190 bp
  • A to G, chromosome 10 at 84,674,590 bp
  • A to G, chromosome 10 at 123,010,492 bp
  • A to C, chromosome 10 at 127,245,441 bp
  • A to T, chromosome 10 at 129,660,038 bp
  • A to C, chromosome 11 at 49,327,933 bp
  • A to G, chromosome 11 at 57,781,056 bp
  • T to C, chromosome 11 at 70,689,881 bp
  • G to A, chromosome 11 at 105,972,880 bp
  • A to G, chromosome 11 at 116,049,331 bp
  • T to C, chromosome 11 at 116,064,625 bp
  • G to T, chromosome 11 at 120,816,124 bp
  • A to G, chromosome 12 at 24,500,449 bp
  • C to T, chromosome 12 at 84,789,101 bp
  • T to C, chromosome 13 at 29,691,686 bp
  • C to A, chromosome 13 at 76,055,926 bp
  • A to C, chromosome 13 at 109,740,442 bp
  • A to T, chromosome 14 at 64,737,555 bp
  • G to A, chromosome 14 at 121,484,871 bp
  • A to G, chromosome 15 at 28,290,195 bp
  • C to T, chromosome 16 at 3,953,995 bp
  • T to C, chromosome 16 at 21,221,687 bp
  • C to A, chromosome 16 at 44,766,034 bp
  • T to A, chromosome 17 at 24,580,212 bp
  • T to A, chromosome 17 at 24,613,216 bp
  • T to A, chromosome 17 at 27,125,886 bp
  • C to T, chromosome 17 at 34,193,305 bp
  • T to C, chromosome 17 at 36,862,840 bp
  • C to T, chromosome 17 at 86,823,686 bp
  • T to C, chromosome 19 at 6,998,300 bp
  • T to A, chromosome 19 at 13,502,558 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5838 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044058-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.