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Strain Name:
C57BL/6J-MtgxR5840Btlr
Stock Number:
044060-MU
Citation ID:
RRID:MMRRC_044060-MU
Other Names:
R5840 (G1)
Major Collection:

Strain Information

Lamb1
Name: laminin B1
Synonyms: Lamb-1, C81607, C80098, D130003D08Rik, Lamb1-1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 16777
HGNC: HGNC:6486
Homologene: 1722
Frem2
Name: Fras1 related extracellular matrix protein 2
Synonyms: 8430406N05Rik, 6030440P17Rik, my, ne, b2b1562Clo
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 242022
Homologene: 18454
Dip2c
Name: disco interacting protein 2 homolog C
Synonyms: 2900024P20Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 208440
Homologene: 40996
Gtf2a2
Name: general transcription factor II A, 2
Synonyms: TfIIg, 12kDa, Tfiia2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235459
HGNC: HGNC:4647
Homologene: 3304
Sema4b
Name: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B
Synonyms: SemC, Semac
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20352
Homologene: 8426
Trpm7
Name: transient receptor potential cation channel, subfamily M, member 7
Synonyms: 5033407O22Rik, 4833414K03Rik, Ltpr7, CHAK1, CHAK, TRP-PLIK, 2310022G15Rik, LTRPC7
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 58800
Homologene: 9774
Dab2ip
Name: disabled 2 interacting protein
Synonyms: 2310011D08Rik, AIP1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 69601
Homologene: 13058
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 58,919,273 bp
  • A to G, chromosome 1 at 66,770,215 bp
  • T to A, chromosome 1 at 128,259,525 bp
  • T to C, chromosome 1 at 156,348,958 bp
  • G to A, chromosome 2 at 25,433,359 bp
  • G to A, chromosome 2 at 30,981,857 bp
  • A to G, chromosome 2 at 35,727,499 bp
  • A to G, chromosome 2 at 121,085,660 bp
  • A to T, chromosome 2 at 126,822,611 bp
  • A to C, chromosome 2 at 128,607,037 bp
  • A to C, chromosome 2 at 128,929,904 bp
  • A to T, chromosome 2 at 139,547,206 bp
  • A to T, chromosome 2 at 165,295,552 bp
  • G to A, chromosome 3 at 30,936,583 bp
  • T to A, chromosome 3 at 53,647,921 bp
  • A to G, chromosome 4 at 48,396,284 bp
  • T to G, chromosome 4 at 140,712,138 bp
  • T to G, chromosome 5 at 65,808,094 bp
  • T to G, chromosome 5 at 115,331,465 bp
  • A to G, chromosome 6 at 41,202,858 bp
  • C to T, chromosome 6 at 41,615,573 bp
  • A to G, chromosome 7 at 18,346,602 bp
  • T to A, chromosome 7 at 23,660,175 bp
  • T to G, chromosome 7 at 24,758,070 bp
  • T to C, chromosome 7 at 75,866,692 bp
  • T to C, chromosome 7 at 80,218,949 bp
  • C to A, chromosome 7 at 81,213,965 bp
  • T to C, chromosome 7 at 87,360,793 bp
  • T to A, chromosome 7 at 103,358,369 bp
  • C to T, chromosome 7 at 103,639,256 bp
  • T to C, chromosome 7 at 104,128,954 bp
  • A to G, chromosome 8 at 91,666,440 bp
  • T to C, chromosome 8 at 123,208,100 bp
  • C to T, chromosome 9 at 9,046,517 bp
  • T to C, chromosome 9 at 27,094,758 bp
  • T to C, chromosome 9 at 35,210,139 bp
  • A to G, chromosome 9 at 70,015,293 bp
  • C to T, chromosome 10 at 77,988,724 bp
  • T to A, chromosome 11 at 4,816,146 bp
  • A to G, chromosome 11 at 26,534,314 bp
  • C to T, chromosome 11 at 102,461,331 bp
  • T to C, chromosome 12 at 10,377,171 bp
  • T to A, chromosome 12 at 31,266,756 bp
  • T to A, chromosome 12 at 75,880,291 bp
  • A to T, chromosome 13 at 9,506,676 bp
  • T to C, chromosome 13 at 64,118,413 bp
  • C to T, chromosome 13 at 65,297,278 bp
  • A to G, chromosome 14 at 19,875,814 bp
  • T to C, chromosome 14 at 37,069,005 bp
  • A to T, chromosome 14 at 51,038,988 bp
  • A to T, chromosome 17 at 14,681,430 bp
  • G to A, chromosome 17 at 25,840,058 bp
  • T to A, chromosome 18 at 9,726,528 bp
  • T to C, chromosome 18 at 37,448,750 bp
  • T to C, chromosome 18 at 54,898,906 bp
  • G to A, chromosome 19 at 4,116,010 bp
  • G to A, chromosome 19 at 6,090,369 bp
  • C to A, chromosome 19 at 7,022,068 bp
  • A to T, chromosome 19 at 7,230,008 bp
  • G to A, chromosome 19 at 13,471,878 bp
  • G to T, chromosome 19 at 42,598,715 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5840 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044060-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.