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Strain Name:
C57BL/6J-MtgxR5909Btlr/Mmmh
Stock Number:
044106-MU
Citation ID:
RRID:MMRRC_044106-MU
Other Names:
R5909 (G1)
Major Collection:

Strain Information

Fancd2
Name: Fanconi anemia, complementation group D2
Synonyms: 2410150O07Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 211651
HGNC: HGNC:3585
Homologene: 13212
Ylpm1
Name: YLP motif containing 1
Synonyms: ZAP, Zap3, A930013E17Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 56531
Homologene: 87707
Plxnd1
Name: plexin D1
Synonyms: 6230425C21Rik, b2b553Clo, b2b1863Clo
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 67784
HGNC: HGNC:9107
Homologene: 22866
Rbm25
Name: RNA binding motif protein 25
Synonyms: 2610015J01Rik, A130095G20Rik, 2600011C06Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 67039
Naa15
Name: N(alpha)-acetyltransferase 15, NatA auxiliary subunit
Synonyms: tubedown, Tbdn-1, mNAT1, 5730450D16Rik, Narg1, ASTBDN
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 74838
Homologene: 14211
Rcc2
Name: regulator of chromosome condensation 2
Synonyms: 2610529N02Rik, 2610510H01Rik, Td60
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 108911
Homologene: 10282
Lrch4
Name: leucine-rich repeats and calponin homology (CH) domain containing 4
Synonyms: 2900069C24Rik, LRRN4, LRN, 2810008P14Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231798
HGNC: HGNC:6691
Homologene: 20532
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 12,858,815 bp
  • A to G, chromosome 1 at 133,383,889 bp
  • A to C, chromosome 1 at 158,601,937 bp
  • A to T, chromosome 1 at 172,287,230 bp
  • A to G, chromosome 2 at 31,048,199 bp
  • A to T, chromosome 2 at 88,402,191 bp
  • A to G, chromosome 2 at 89,844,391 bp
  • A to T, chromosome 2 at 131,077,964 bp
  • T to C, chromosome 3 at 9,280,049 bp
  • T to C, chromosome 3 at 51,460,064 bp
  • G to T, chromosome 4 at 21,683,894 bp
  • C to T, chromosome 4 at 46,629,912 bp
  • T to G, chromosome 4 at 62,086,007 bp
  • A to T, chromosome 4 at 124,857,173 bp
  • T to A, chromosome 4 at 140,717,068 bp
  • A to T, chromosome 5 at 120,788,907 bp
  • T to C, chromosome 5 at 124,566,850 bp
  • T to C, chromosome 5 at 137,633,865 bp
  • A to G, chromosome 6 at 32,517,246 bp
  • T to C, chromosome 6 at 53,932,428 bp
  • T to A, chromosome 6 at 113,561,711 bp
  • G to T, chromosome 6 at 115,968,688 bp
  • A to G, chromosome 6 at 143,197,438 bp
  • G to A, chromosome 7 at 3,897,622 bp
  • A to T, chromosome 7 at 10,274,342 bp
  • G to T, chromosome 7 at 25,308,193 bp
  • G to A, chromosome 7 at 44,507,091 bp
  • A to C, chromosome 7 at 141,441,270 bp
  • A to G, chromosome 8 at 71,684,231 bp
  • A to G, chromosome 8 at 74,892,798 bp
  • G to T, chromosome 8 at 86,021,447 bp
  • A to T, chromosome 8 at 88,325,496 bp
  • G to A, chromosome 8 at 117,024,056 bp
  • T to C, chromosome 9 at 30,961,928 bp
  • G to T, chromosome 9 at 62,060,144 bp
  • A to T, chromosome 9 at 89,085,398 bp
  • G to A, chromosome 10 at 39,072,859 bp
  • T to C, chromosome 10 at 41,561,115 bp
  • A to C, chromosome 10 at 60,772,359 bp
  • G to A, chromosome 11 at 35,798,332 bp
  • A to T, chromosome 11 at 58,968,680 bp
  • T to A, chromosome 11 at 78,540,041 bp
  • A to T, chromosome 11 at 100,095,813 bp
  • G to A, chromosome 11 at 100,903,730 bp
  • T to A, chromosome 12 at 83,681,588 bp
  • C to T, chromosome 12 at 85,040,886 bp
  • C to A, chromosome 12 at 111,680,858 bp
  • A to G, chromosome 13 at 12,579,258 bp
  • A to G, chromosome 13 at 40,263,578 bp
  • T to A, chromosome 13 at 67,786,598 bp
  • A to G, chromosome 13 at 74,199,524 bp
  • A to G, chromosome 13 at 100,092,286 bp
  • G to T, chromosome 15 at 44,526,763 bp
  • C to T, chromosome 15 at 83,547,915 bp
  • A to T, chromosome 15 at 85,818,296 bp
  • A to G, chromosome 16 at 23,972,806 bp
  • G to T, chromosome 16 at 52,290,993 bp
  • T to C, chromosome 16 at 56,258,136 bp
  • T to C, chromosome 17 at 23,821,317 bp
  • T to A, chromosome 18 at 60,300,019 bp
  • T to C, chromosome 19 at 4,000,727 bp
  • G to A, chromosome Y at 941,306 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5909 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044106-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.