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Strain Name:
C57BL/6J-MtgxR5935Btlr/Mmmh
Stock Number:
044129-MU
Citation ID:
RRID:MMRRC_044129-MU
Other Names:
R5935 (G1)
Major Collection:

Strain Information

Htr2a
Name: 5-hydroxytryptamine (serotonin) receptor 2A
Synonyms: 5-HT2A receptor, Htr-2, Htr2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 15558
VEGA: 14
HGNC: HGNC:5293
Homologene: 68073
Npy2r
Name: neuropeptide Y receptor Y2
Synonyms: NPY-Y2 receptor
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18167
HGNC: HGNC:7957
Homologene: 701
Tnfsf4
Name: tumor necrosis factor (ligand) superfamily, member 4
Synonyms: OX40L, gp34, Txgp1l, Ath-1, TXGP1, CD134L, Ath1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 22164
Homologene: 2495
Scn3a
Name: sodium channel, voltage-gated, type III, alpha
Synonyms: LOC381367, Nav1.3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20269
Homologene: 56005
Ppp1r13b
Name: protein phosphatase 1, regulatory subunit 13B
Synonyms: ASPP1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 21981
VEGA: 12
Homologene: 9090
Mif4gd
Name: MIF4G domain containing
Synonyms: 1110014L05Rik, 2310075G12Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 69674
Homologene: 41389
Camsap3
Name: calmodulin regulated spectrin-associated protein family, member 3
Synonyms: Nezha, 2310057J16Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 69697
Homologene: 18966
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 80,505,587 bp
  • A to C, chromosome 1 at 83,339,599 bp
  • G to A, chromosome 1 at 93,620,645 bp
  • T to C, chromosome 1 at 107,083,375 bp
  • A to G, chromosome 1 at 120,120,853 bp
  • T to A, chromosome 1 at 132,454,137 bp
  • T to A, chromosome 1 at 161,417,248 bp
  • GCC to GCCC, chromosome 2 at 37,414,733 bp
  • G to T, chromosome 2 at 65,464,836 bp
  • A to G, chromosome 2 at 91,615,100 bp
  • A to G, chromosome 2 at 180,262,048 bp
  • A to T, chromosome 3 at 59,328,157 bp
  • A to T, chromosome 3 at 82,540,761 bp
  • T to A, chromosome 3 at 133,488,535 bp
  • C to T, chromosome 3 at 157,947,583 bp
  • T to C, chromosome 4 at 42,971,465 bp
  • C to A, chromosome 4 at 107,857,296 bp
  • T to C, chromosome 4 at 133,419,865 bp
  • C to A, chromosome 4 at 135,856,242 bp
  • T to A, chromosome 5 at 8,974,192 bp
  • A to T, chromosome 5 at 30,673,037 bp
  • A to T, chromosome 5 at 115,925,539 bp
  • T to A, chromosome 5 at 137,443,930 bp
  • T to A, chromosome 5 at 146,090,645 bp
  • A to T, chromosome 6 at 55,897,769 bp
  • T to C, chromosome 6 at 77,143,921 bp
  • A to G, chromosome 6 at 115,559,595 bp
  • A to T, chromosome 6 at 123,741,895 bp
  • A to G, chromosome 6 at 128,408,398 bp
  • A to T, chromosome 7 at 25,059,077 bp
  • T to A, chromosome 7 at 28,329,475 bp
  • C to A, chromosome 7 at 84,943,661 bp
  • T to A, chromosome 7 at 102,948,810 bp
  • A to T, chromosome 7 at 119,471,427 bp
  • A to G, chromosome 7 at 119,859,576 bp
  • G to A, chromosome 7 at 139,976,613 bp
  • C to T, chromosome 7 at 143,756,958 bp
  • A to G, chromosome 8 at 3,601,999 bp
  • T to C, chromosome 8 at 40,464,236 bp
  • C to G, chromosome 8 at 43,521,298 bp
  • A to T, chromosome 8 at 95,319,992 bp
  • G to T, chromosome 8 at 119,947,089 bp
  • G to A, chromosome 8 at 122,476,954 bp
  • A to T, chromosome 8 at 128,713,237 bp
  • T to A, chromosome 9 at 14,786,962 bp
  • A to T, chromosome 9 at 15,010,217 bp
  • C to T, chromosome 9 at 39,669,090 bp
  • C to T, chromosome 9 at 50,917,131 bp
  • T to A, chromosome 9 at 109,056,683 bp
  • T to A, chromosome 9 at 110,966,561 bp
  • G to A, chromosome 9 at 119,627,171 bp
  • T to C, chromosome 10 at 5,360,706 bp
  • T to A, chromosome 10 at 27,015,498 bp
  • A to T, chromosome 10 at 115,431,357 bp
  • A to G, chromosome 11 at 5,959,338 bp
  • C to T, chromosome 11 at 9,052,711 bp
  • G to C, chromosome 11 at 45,944,618 bp
  • A to G, chromosome 11 at 59,006,813 bp
  • A to T, chromosome 11 at 69,761,683 bp
  • A to T, chromosome 11 at 83,882,677 bp
  • G to T, chromosome 11 at 87,865,492 bp
  • A to T, chromosome 11 at 102,025,415 bp
  • C to T, chromosome 11 at 115,609,613 bp
  • A to G, chromosome 12 at 57,673,804 bp
  • T to A, chromosome 12 at 101,051,613 bp
  • T to C, chromosome 12 at 111,830,442 bp
  • T to C, chromosome 13 at 55,320,530 bp
  • T to C, chromosome 13 at 65,037,080 bp
  • T to G, chromosome 14 at 14,350,799 bp
  • C to A, chromosome 14 at 16,434,264 bp
  • A to G, chromosome 14 at 66,173,879 bp
  • A to G, chromosome 14 at 74,645,090 bp
  • T to G, chromosome 14 at 110,749,873 bp
  • C to T, chromosome 15 at 66,793,705 bp
  • C to A, chromosome 15 at 74,621,154 bp
  • C to A, chromosome 15 at 91,745,831 bp
  • T to C, chromosome 15 at 92,397,374 bp
  • A to T, chromosome 15 at 103,294,770 bp
  • A to G, chromosome 15 at 103,521,439 bp
  • A to G, chromosome 16 at 16,309,471 bp
  • A to T, chromosome 16 at 25,317,261 bp
  • G to T, chromosome 17 at 31,632,879 bp
  • A to T, chromosome 17 at 54,276,944 bp
  • A to G, chromosome 18 at 37,356,190 bp
  • C to A, chromosome 19 at 4,086,497 bp
  • T to C, chromosome 19 at 9,015,182 bp
  • T to C, chromosome 19 at 34,619,728 bp
  • C to T, chromosome 19 at 46,654,180 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5935 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044129-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.