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Strain Name:
C57BL/6J-MtgxR5965Btlr/Mmmh
Stock Number:
044150-MU
Citation ID:
RRID:MMRRC_044150-MU
Other Names:
R5965 (G1)
Major Collection:

Strain Information

Derl2
Name: Der1-like domain family, member 2
Synonyms: CGI-101, F-lana, Flana, Derlin-2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 116891
Homologene: 9356
Lamb3
Name: laminin, beta 3
Synonyms: nicein, 125kDa
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16780
HGNC: HGNC:6490
Homologene: 191
Scd1
Name: stearoyl-Coenzyme A desaturase 1
Synonyms: stearoyl-CoA desaturase, Scd-1, SCD
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 20249
VEGA: 19
Homologene: 74538
Nfia
Name: nuclear factor I/A
Synonyms: 1110047K16Rik, NF1-A, 9430022M17Rik, NF1A
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18027
HGNC: HGNC:7784
Homologene: 4086
Snx2
Name: sorting nexin 2
Synonyms: 0610030A03Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 67804
VEGA: 18
Homologene: 2332
Ranbp1
Name: RAN binding protein 1
Synonyms: Htf9a
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19385
HGNC: HGNC:9847
Homologene: 21600
Kdm3a
Name: lysine (K)-specific demethylase 3A
Synonyms: 1700105C21Rik, Tsga, C230043E16Rik, Jmjd1, Jmjd1a
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 104263
Homologene: 10196
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 36,345,520 bp
  • T to C, chromosome 1 at 46,362,987 bp
  • T to C, chromosome 1 at 80,568,744 bp
  • A to G, chromosome 1 at 107,276,923 bp
  • T to G, chromosome 1 at 119,526,564 bp
  • A to T, chromosome 1 at 193,343,460 bp
  • A to G, chromosome 2 at 11,908,505 bp
  • A to T, chromosome 2 at 26,222,221 bp
  • T to G, chromosome 2 at 87,458,037 bp
  • T to A, chromosome 3 at 50,379,144 bp
  • A to C, chromosome 3 at 95,748,152 bp
  • T to A, chromosome 3 at 145,578,348 bp
  • T to C, chromosome 4 at 98,111,292 bp
  • T to C, chromosome 4 at 108,691,681 bp
  • C to T, chromosome 4 at 119,248,227 bp
  • T to A, chromosome 5 at 30,205,639 bp
  • T to A, chromosome 5 at 104,021,785 bp
  • T to C, chromosome 5 at 143,264,672 bp
  • A to T, chromosome 6 at 41,152,055 bp
  • C to A, chromosome 6 at 52,729,332 bp
  • A to G, chromosome 6 at 71,621,380 bp
  • T to A, chromosome 6 at 118,602,300 bp
  • T to A, chromosome 6 at 125,152,508 bp
  • T to C, chromosome 7 at 4,029,024 bp
  • T to C, chromosome 7 at 15,952,074 bp
  • C to T, chromosome 7 at 45,455,339 bp
  • C to A, chromosome 7 at 102,724,260 bp
  • T to G, chromosome 7 at 105,755,925 bp
  • T to A, chromosome 7 at 142,490,424 bp
  • C to A, chromosome 7 at 145,307,431 bp
  • T to C, chromosome 8 at 25,735,777 bp
  • C to A, chromosome 8 at 48,228,508 bp
  • T to C, chromosome 8 at 57,857,531 bp
  • A to T, chromosome 8 at 70,452,731 bp
  • T to C, chromosome 8 at 105,234,539 bp
  • T to C, chromosome 8 at 123,316,410 bp
  • T to A, chromosome 9 at 18,409,542 bp
  • A to C, chromosome 9 at 44,136,628 bp
  • C to A, chromosome 10 at 14,766,810 bp
  • A to G, chromosome 10 at 41,922,572 bp
  • A to G, chromosome 10 at 53,933,490 bp
  • A to G, chromosome 11 at 42,626,869 bp
  • A to G, chromosome 11 at 69,914,794 bp
  • T to C, chromosome 11 at 71,014,552 bp
  • A to T, chromosome 11 at 72,400,453 bp
  • T to A, chromosome 12 at 78,710,306 bp
  • T to A, chromosome 12 at 110,632,778 bp
  • T to C, chromosome 12 at 115,131,557 bp
  • A to G, chromosome 14 at 49,034,931 bp
  • A to C, chromosome 14 at 52,638,797 bp
  • A to T, chromosome 14 at 56,624,032 bp
  • A to G, chromosome 15 at 54,882,971 bp
  • T to C, chromosome 15 at 63,804,598 bp
  • T to A, chromosome 15 at 99,945,097 bp
  • A to G, chromosome 16 at 4,087,661 bp
  • C to T, chromosome 16 at 17,530,747 bp
  • T to C, chromosome 16 at 18,245,228 bp
  • T to C, chromosome 16 at 59,086,303 bp
  • T to A, chromosome 17 at 23,670,868 bp
  • T to A, chromosome 17 at 32,524,637 bp
  • T to C, chromosome 18 at 10,593,867 bp
  • A to G, chromosome 18 at 12,429,887 bp
  • C to T, chromosome 18 at 30,298,580 bp
  • G to T, chromosome 18 at 53,194,462 bp
  • A to G, chromosome 18 at 60,833,418 bp
  • T to G, chromosome 19 at 4,883,750 bp
  • T to C, chromosome 19 at 16,619,028 bp
  • G to A, chromosome 19 at 29,570,771 bp
  • T to C, chromosome 19 at 30,724,156 bp
  • A to G, chromosome 19 at 44,400,140 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5965 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044150-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.