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Strain Name:
C57BL/6J-MtgxR5969Btlr/Mmmh
Stock Number:
044152-MU
Citation ID:
RRID:MMRRC_044152-MU
Other Names:
R5969 (G1)
Major Collection:

Strain Information

Notch3
Name: notch 3
Synonyms: hpbk, N3
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18131
HGNC: HGNC:7883
Homologene: 376
Lyst
Name: lysosomal trafficking regulator
Synonyms: D13Sfk13
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17101
VEGA: 13
HGNC: HGNC:1968
Homologene: 61
Fxyd2
Name: FXYD domain-containing ion transport regulator 2
Synonyms: Atp1g1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 11936
VEGA: 9
HGNC: HGNC:4026
Ece1
Name: endothelin converting enzyme 1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230857
HGNC: HGNC:3146
Homologene: 1068
Phf21a
Name: PHD finger protein 21A
Synonyms: 80kDa, Braf35/HDAC complex (Bhc), PFTF1, Bhc80, D030065N23Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 192285
Homologene: 9597
Cxcr4
Name: C-X-C motif chemokine receptor 4
Synonyms: PB-CKR, fusin, CD184, Cmkar4, Sdf1r, b2b220Clo
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12767
HGNC: HGNC:2561
Homologene: 20739
Kif20a
Name: kinesin family member 20A
Synonyms: Rabkinesin-6, Rab6kifl
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 19348
VEGA: 18
HGNC: HGNC:9787
Homologene: 38093
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 10,488,883 bp
  • T to C, chromosome 1 at 75,549,979 bp
  • T to C, chromosome 1 at 92,983,220 bp
  • T to G, chromosome 1 at 119,527,463 bp
  • T to A, chromosome 1 at 128,589,847 bp
  • T to C, chromosome 2 at 28,542,414 bp
  • T to G, chromosome 2 at 69,979,386 bp
  • A to T, chromosome 2 at 92,221,611 bp
  • G to T, chromosome 2 at 158,217,708 bp
  • A to T, chromosome 3 at 10,438,157 bp
  • C to T, chromosome 3 at 28,620,948 bp
  • T to A, chromosome 3 at 59,115,158 bp
  • T to A, chromosome 3 at 79,597,717 bp
  • G to A, chromosome 4 at 58,070,977 bp
  • T to A, chromosome 4 at 134,075,692 bp
  • T to C, chromosome 4 at 137,961,740 bp
  • C to T, chromosome 6 at 35,177,578 bp
  • A to G, chromosome 6 at 58,965,198 bp
  • G to A, chromosome 6 at 123,779,022 bp
  • C to A, chromosome 7 at 42,489,488 bp
  • A to G, chromosome 7 at 43,784,985 bp
  • T to A, chromosome 7 at 64,744,447 bp
  • C to T, chromosome 7 at 92,917,766 bp
  • T to A, chromosome 7 at 103,906,910 bp
  • A to G, chromosome 7 at 125,645,676 bp
  • T to A, chromosome 8 at 16,071,353 bp
  • C to A, chromosome 8 at 116,940,537 bp
  • C to T, chromosome 9 at 26,780,742 bp
  • T to A, chromosome 9 at 45,408,330 bp
  • T to C, chromosome 9 at 57,713,428 bp
  • A to G, chromosome 9 at 78,452,905 bp
  • A to T, chromosome 10 at 20,984,393 bp
  • T to A, chromosome 11 at 9,292,214 bp
  • T to C, chromosome 11 at 54,063,656 bp
  • T to C, chromosome 12 at 51,959,040 bp
  • T to C, chromosome 12 at 87,919,478 bp
  • T to C, chromosome 12 at 101,043,579 bp
  • T to C, chromosome 12 at 102,405,827 bp
  • A to C, chromosome 13 at 13,687,813 bp
  • G to A, chromosome 13 at 74,172,186 bp
  • A to T, chromosome 13 at 93,089,544 bp
  • A to G, chromosome 13 at 110,353,946 bp
  • G to A, chromosome 14 at 21,670,792 bp
  • G to A, chromosome 15 at 36,015,636 bp
  • G to T, chromosome 15 at 47,947,990 bp
  • A to T, chromosome 15 at 78,764,382 bp
  • T to A, chromosome 15 at 78,967,540 bp
  • A to C, chromosome 15 at 102,350,564 bp
  • T to C, chromosome 16 at 16,883,565 bp
  • A to G, chromosome 16 at 65,839,563 bp
  • C to A, chromosome 17 at 32,153,884 bp
  • C to T, chromosome 17 at 36,882,427 bp
  • A to G, chromosome 17 at 64,625,380 bp
  • G to A, chromosome 18 at 34,632,415 bp
  • A to T, chromosome 18 at 36,600,834 bp
  • C to T, chromosome 18 at 37,959,873 bp
  • T to C, chromosome 19 at 5,685,535 bp
  • T to C, chromosome 19 at 9,016,585 bp
  • T to C, chromosome 19 at 43,491,472 bp
  • C to T, chromosome 19 at 57,384,123 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5969 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044152-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.