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Strain Name:
C57BL/6J-MtgxR5987Btlr/Mmmh
Stock Number:
044167-MU
Citation ID:
RRID:MMRRC_044167-MU
Other Names:
R5987 (G1)
Major Collection:

Strain Information

Gba1
Name: glucosylceramidase beta 1
Synonyms: glucocerebrosidase, GC, GCase, GBA1, betaGC, Gba
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14466
HGNC: HGNC:4177
Homologene: 68040
Ptch2
Name: patched 2
Synonyms: ptc2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 19207
HGNC: HGNC:9586
Homologene: 37842
Areg
Name: amphiregulin
Synonyms: AR, Sdgf, Mcub
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 11839
HGNC: HGNC:651
Homologene: 1252
Abca12
Name: ATP-binding cassette, sub-family A member 12
Synonyms: 4832428G11Rik, 4833417A11Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74591
Homologene: 45441
E2f2
Name: E2F transcription factor 2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242705
HGNC: HGNC:3114
Homologene: 48264
Rgs12
Name: regulator of G-protein signaling 12
Synonyms: 4632412M04Rik, 1200016K18Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 71729
HGNC: HGNC:9994
Homologene: 2195
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 44,057,257 bp
  • A to G, chromosome 1 at 46,119,398 bp
  • C to A, chromosome 1 at 58,307,359 bp
  • A to G, chromosome 1 at 59,206,587 bp
  • A to C, chromosome 1 at 71,258,098 bp
  • G to A, chromosome 1 at 163,996,775 bp
  • G to T, chromosome 1 at 174,223,328 bp
  • A to T, chromosome 2 at 25,203,888 bp
  • C to G, chromosome 2 at 52,095,844 bp
  • G to T, chromosome 2 at 52,295,294 bp
  • C to CGTG, chromosome 2 at 98,663,807 bp
  • T to C, chromosome 2 at 121,304,295 bp
  • C to A, chromosome 2 at 121,350,491 bp
  • A to G, chromosome 2 at 128,771,374 bp
  • T to C, chromosome 3 at 30,896,463 bp
  • A to G, chromosome 3 at 55,126,541 bp
  • T to G, chromosome 3 at 73,648,678 bp
  • T to C, chromosome 3 at 89,205,822 bp
  • T to C, chromosome 3 at 94,779,522 bp
  • C to A, chromosome 3 at 100,202,256 bp
  • T to C, chromosome 3 at 132,801,517 bp
  • T to C, chromosome 3 at 133,206,958 bp
  • A to T, chromosome 4 at 6,268,476 bp
  • C to T, chromosome 4 at 41,632,391 bp
  • A to G, chromosome 4 at 110,290,644 bp
  • T to C, chromosome 4 at 113,885,808 bp
  • C to T, chromosome 4 at 117,110,057 bp
  • C to A, chromosome 4 at 117,680,842 bp
  • T to C, chromosome 4 at 118,830,281 bp
  • A to G, chromosome 4 at 119,246,665 bp
  • A to T, chromosome 4 at 136,172,934 bp
  • T to C, chromosome 4 at 141,464,817 bp
  • A to G, chromosome 4 at 156,349,997 bp
  • T to C, chromosome 5 at 29,203,147 bp
  • A to G, chromosome 5 at 35,020,345 bp
  • C to A, chromosome 5 at 45,454,027 bp
  • A to T, chromosome 5 at 91,146,718 bp
  • T to A, chromosome 6 at 85,959,233 bp
  • T to A, chromosome 6 at 136,571,502 bp
  • T to C, chromosome 7 at 16,563,467 bp
  • G to A, chromosome 7 at 24,105,434 bp
  • T to C, chromosome 7 at 45,438,105 bp
  • T to C, chromosome 7 at 62,378,767 bp
  • T to A, chromosome 7 at 103,881,700 bp
  • T to A, chromosome 7 at 108,323,047 bp
  • T to C, chromosome 7 at 112,150,658 bp
  • C to T, chromosome 7 at 112,334,948 bp
  • A to T, chromosome 8 at 33,776,052 bp
  • G to T, chromosome 8 at 33,776,053 bp
  • T to A, chromosome 8 at 70,321,578 bp
  • A to T, chromosome 8 at 119,626,359 bp
  • A to G, chromosome 8 at 128,476,169 bp
  • T to C, chromosome 9 at 21,962,276 bp
  • A to G, chromosome 9 at 37,411,400 bp
  • T to C, chromosome 9 at 39,772,540 bp
  • A to G, chromosome 9 at 57,127,200 bp
  • T to A, chromosome 9 at 110,381,151 bp
  • A to G, chromosome 9 at 122,853,886 bp
  • G to T, chromosome 10 at 58,255,847 bp
  • A to T, chromosome 10 at 116,972,592 bp
  • T to G, chromosome 10 at 128,923,386 bp
  • A to T, chromosome 10 at 129,874,521 bp
  • G to A, chromosome 11 at 69,967,668 bp
  • A to G, chromosome 11 at 78,080,233 bp
  • C to T, chromosome 12 at 69,303,613 bp
  • C to T, chromosome 13 at 14,637,780 bp
  • A to T, chromosome 13 at 19,340,304 bp
  • A to G, chromosome 13 at 21,484,453 bp
  • A to G, chromosome 13 at 23,757,226 bp
  • A to G, chromosome 13 at 67,317,624 bp
  • A to T, chromosome 13 at 97,936,860 bp
  • T to A, chromosome 13 at 102,758,734 bp
  • A to G, chromosome 14 at 26,669,098 bp
  • C to A, chromosome 14 at 26,886,871 bp
  • T to A, chromosome 14 at 75,260,688 bp
  • G to A, chromosome 15 at 30,683,241 bp
  • A to C, chromosome 15 at 63,830,866 bp
  • A to G, chromosome 15 at 71,490,848 bp
  • C to T, chromosome 15 at 100,190,079 bp
  • T to C, chromosome 16 at 17,276,470 bp
  • A to T, chromosome 16 at 46,464,145 bp
  • A to G, chromosome 16 at 57,302,245 bp
  • A to G, chromosome 17 at 23,739,858 bp
  • T to A, chromosome 17 at 27,104,601 bp
  • T to A, chromosome 17 at 37,796,357 bp
  • A to G, chromosome 17 at 88,755,578 bp
  • T to A, chromosome 18 at 20,331,542 bp
  • T to A, chromosome 18 at 24,686,693 bp
  • G to T, chromosome 18 at 56,957,896 bp
  • C to G, chromosome 19 at 3,628,299 bp
  • G to A, chromosome 19 at 7,373,659 bp
  • A to T, chromosome 19 at 13,453,960 bp
  • A to C, chromosome 19 at 34,612,198 bp
  • A to G, chromosome 19 at 40,929,289 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5987 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044167-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.