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Strain Name:
C57BL/6J-MtgxR5996Btlr/Mmmh
Stock Number:
044175-MU
Citation ID:
RRID:MMRRC_044175-MU
Other Names:
R5996 (G1)
Major Collection:

Strain Information

Uso1
Name: USO1 vesicle docking factor
Synonyms: transcytosis associated protein p115, TAP, Vdp
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 56041
Homologene: 2754
Map2k6
Name: mitogen-activated protein kinase kinase 6
Synonyms: MAP kinase kinase 6, MKK6, SAPKK3, Prkmk6
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 26399
HGNC: HGNC:6846
Homologene: 55686
Tm9sf4
Name: transmembrane 9 superfamily member 4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 99237
Homologene: 21620
Dlg4
Name: discs large MAGUK scaffold protein 4
Synonyms: SAP90, PSD-95, SAP90A, PSD95, Dlgh4
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 13385
HGNC: HGNC:2903
Homologene: 1047
Gtse1
Name: G two S phase expressed protein 1
Synonyms: B99, Gtse-1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 29870
VEGA: 15
Homologene: 8489
Xrcc5
Name: X-ray repair complementing defective repair in Chinese hamster cells 5
Synonyms: Ku80, Ku86
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 22596
VEGA: 1
Homologene: 40681
Cep57
Name: centrosomal protein 57
Synonyms: 4931428M20Rik, 4921510P06Rik, Tsp57, 3110002L15Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74360
Homologene: 8790
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 24,328,071 bp
  • A to G, chromosome 1 at 51,471,385 bp
  • C to A, chromosome 1 at 66,414,884 bp
  • T to A, chromosome 1 at 72,310,458 bp
  • T to C, chromosome 1 at 74,158,460 bp
  • T to C, chromosome 1 at 82,455,728 bp
  • T to G, chromosome 1 at 171,581,640 bp
  • T to A, chromosome 1 at 171,601,828 bp
  • T to A, chromosome 2 at 3,779,630 bp
  • G to A, chromosome 2 at 63,184,097 bp
  • T to A, chromosome 2 at 67,511,650 bp
  • A to C, chromosome 2 at 118,283,904 bp
  • T to C, chromosome 2 at 127,577,048 bp
  • T to C, chromosome 2 at 153,195,571 bp
  • T to C, chromosome 3 at 31,209,236 bp
  • T to C, chromosome 3 at 36,931,116 bp
  • T to C, chromosome 4 at 107,066,507 bp
  • A to C, chromosome 4 at 108,719,360 bp
  • A to G, chromosome 4 at 110,955,672 bp
  • A to C, chromosome 4 at 152,434,830 bp
  • C to T, chromosome 5 at 24,400,139 bp
  • A to T, chromosome 5 at 37,159,824 bp
  • A to T, chromosome 5 at 92,192,730 bp
  • C to T, chromosome 6 at 48,494,176 bp
  • T to A, chromosome 6 at 121,659,394 bp
  • A to G, chromosome 6 at 149,068,095 bp
  • T to C, chromosome 7 at 29,024,241 bp
  • A to G, chromosome 7 at 79,111,320 bp
  • A to T, chromosome 7 at 85,411,909 bp
  • T to C, chromosome 7 at 125,567,221 bp
  • T to C, chromosome 7 at 130,623,483 bp
  • C to A, chromosome 7 at 141,259,102 bp
  • T to C, chromosome 8 at 3,918,688 bp
  • A to T, chromosome 8 at 47,203,147 bp
  • A to T, chromosome 8 at 78,276,896 bp
  • A to G, chromosome 8 at 105,773,040 bp
  • A to G, chromosome 8 at 105,887,401 bp
  • C to T, chromosome 9 at 7,274,269 bp
  • A to G, chromosome 9 at 13,809,879 bp
  • A to T, chromosome 9 at 49,278,670 bp
  • A to G, chromosome 9 at 62,755,673 bp
  • T to A, chromosome 9 at 120,094,463 bp
  • T to C, chromosome 10 at 60,413,577 bp
  • G to A, chromosome 10 at 84,840,017 bp
  • A to G, chromosome 11 at 23,518,928 bp
  • A to T, chromosome 11 at 49,651,070 bp
  • G to A, chromosome 11 at 70,017,231 bp
  • T to C, chromosome 11 at 77,466,496 bp
  • A to G, chromosome 11 at 82,987,484 bp
  • C to T, chromosome 11 at 94,513,594 bp
  • A to G, chromosome 11 at 110,497,906 bp
  • A to G, chromosome 12 at 28,747,114 bp
  • A to G, chromosome 12 at 66,797,763 bp
  • A to G, chromosome 12 at 76,439,162 bp
  • A to G, chromosome 13 at 67,593,858 bp
  • G to T, chromosome 14 at 31,139,551 bp
  • A to G, chromosome 14 at 50,345,512 bp
  • C to T, chromosome 14 at 51,364,348 bp
  • C to T, chromosome 15 at 6,435,311 bp
  • T to C, chromosome 15 at 85,864,180 bp
  • A to G, chromosome 16 at 21,905,245 bp
  • A to G, chromosome 16 at 44,384,674 bp
  • A to T, chromosome 16 at 62,769,686 bp
  • C to T, chromosome 17 at 32,929,473 bp
  • T to C, chromosome 17 at 46,431,284 bp
  • C to A, chromosome 17 at 46,714,458 bp
  • T to A, chromosome 17 at 71,704,783 bp
  • T to C, chromosome 17 at 71,856,310 bp
  • G to T, chromosome 18 at 32,462,492 bp
  • A to T, chromosome 18 at 37,685,938 bp
  • A to G, chromosome 18 at 58,612,234 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5996 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044175-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.