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Strain Name:
C57BL/6J-MtgxR6058Btlr/Mmmh
Stock Number:
044224-MU
Citation ID:
RRID:MMRRC_044224-MU
Other Names:
R6058 (G1)
Major Collection:

Strain Information

Adamts20
Name: ADAM metallopeptidase with thrombospondin type 1 motif 20
Synonyms: ADAMTS-20, bt
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223838
Homologene: 11808
Otx2
Name: orthodenticle homeobox 2
Synonyms: E130306E05Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 18424
HGNC: HGNC:8522
Homologene: 11026
Ascl1
Name: achaete-scute family bHLH transcription factor 1
Synonyms: ASH1, Mash1, bHLHa46
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17172
HGNC: HGNC:738
Homologene: 31234
Cd86
Name: CD86 antigen
Synonyms: B7-2, MB7-2, B70, Ly-58, Cd28l2, B7.2, Ly58
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 12524
HGNC: HGNC:1705
Homologene: 10443
Ldb2
Name: LIM domain binding 2
Synonyms: CLIM1, Ldb3, CLP-36, CLIM-1b, CLIM-1a
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 16826
HGNC: HGNC:6533
Homologene: 989
Gpm6a
Name: glycoprotein m6a
Synonyms: M6A, Gpm6
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234267
HGNC: HGNC:4460
Homologene: 3864
Bahcc1
Name: BAH domain and coiled-coil containing 1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 268515
Homologene: 129585
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 53,923,976 bp
  • T to A, chromosome 1 at 66,415,414 bp
  • A to G, chromosome 1 at 87,211,352 bp
  • A to T, chromosome 1 at 120,476,706 bp
  • C to T, chromosome 1 at 150,451,446 bp
  • C to T, chromosome 1 at 153,136,829 bp
  • T to C, chromosome 1 at 153,907,341 bp
  • T to C, chromosome 1 at 156,524,855 bp
  • G to A, chromosome 1 at 172,484,889 bp
  • G to A, chromosome 2 at 32,560,090 bp
  • A to G, chromosome 2 at 68,861,664 bp
  • A to G, chromosome 2 at 76,916,678 bp
  • A to G, chromosome 2 at 125,466,612 bp
  • T to A, chromosome 2 at 140,240,969 bp
  • A to T, chromosome 3 at 64,725,015 bp
  • T to C, chromosome 4 at 134,058,731 bp
  • A to T, chromosome 4 at 150,468,798 bp
  • T to C, chromosome 5 at 44,476,563 bp
  • T to A, chromosome 5 at 64,278,009 bp
  • A to G, chromosome 5 at 96,709,985 bp
  • T to A, chromosome 5 at 124,885,978 bp
  • A to G, chromosome 6 at 118,179,319 bp
  • T to C, chromosome 7 at 4,920,918 bp
  • T to C, chromosome 7 at 19,370,575 bp
  • T to C, chromosome 7 at 19,426,385 bp
  • TGG to TG, chromosome 7 at 21,272,219 bp
  • G to A, chromosome 7 at 104,148,752 bp
  • C to T, chromosome 7 at 105,611,071 bp
  • G to A, chromosome 7 at 140,812,097 bp
  • T to C, chromosome 8 at 55,058,798 bp
  • G to T, chromosome 8 at 105,523,334 bp
  • A to T, chromosome 9 at 13,810,761 bp
  • T to A, chromosome 9 at 54,772,845 bp
  • A to G, chromosome 10 at 4,453,488 bp
  • A to T, chromosome 10 at 41,740,922 bp
  • T to A, chromosome 10 at 63,275,042 bp
  • T to G, chromosome 10 at 87,492,700 bp
  • T to C, chromosome 10 at 107,635,274 bp
  • T to C, chromosome 10 at 129,238,460 bp
  • C to T, chromosome 11 at 30,426,349 bp
  • T to C, chromosome 11 at 33,642,478 bp
  • A to T, chromosome 11 at 53,463,481 bp
  • A to G, chromosome 11 at 70,611,720 bp
  • C to T, chromosome 11 at 116,073,568 bp
  • T to G, chromosome 11 at 120,287,385 bp
  • T to A, chromosome 12 at 88,177,225 bp
  • A to G, chromosome 12 at 112,662,200 bp
  • T to C, chromosome 13 at 25,056,371 bp
  • T to C, chromosome 13 at 56,480,682 bp
  • T to C, chromosome 14 at 48,658,758 bp
  • T to C, chromosome 14 at 50,618,701 bp
  • C to T, chromosome 14 at 117,964,770 bp
  • T to C, chromosome 15 at 75,618,523 bp
  • T to C, chromosome 15 at 81,667,354 bp
  • T to C, chromosome 15 at 89,253,252 bp
  • A to G, chromosome 15 at 93,387,582 bp
  • G to A, chromosome 15 at 94,330,047 bp
  • T to C, chromosome 16 at 36,629,015 bp
  • T to C, chromosome 16 at 59,085,910 bp
  • A to G, chromosome 16 at 59,086,429 bp
  • C to A, chromosome 16 at 92,079,075 bp
  • A to T, chromosome 17 at 17,443,750 bp
  • G to T, chromosome 17 at 33,999,330 bp
  • T to C, chromosome 17 at 33,999,331 bp
  • A to G, chromosome 17 at 38,275,259 bp
  • T to A, chromosome 17 at 71,869,747 bp
  • T to A, chromosome 17 at 73,906,269 bp
  • T to C, chromosome 18 at 5,213,209 bp
  • A to T, chromosome 18 at 37,675,088 bp
  • A to T, chromosome 18 at 42,327,505 bp
  • A to T, chromosome 18 at 43,728,182 bp
  • G to A, chromosome 19 at 8,767,822 bp
  • T to A, chromosome 19 at 11,896,024 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6058 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044224-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.