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Strain Name:
C57BL/6J-MtgxR6062Btlr/Mmmh
Stock Number:
044227-MU
Citation ID:
RRID:MMRRC_044227-MU
Other Names:
R6062 (G1)
Major Collection:

Strain Information

Gabrg1
Name: gamma-aminobutyric acid type A receptor subunit gamma 1
Synonyms: GabaA
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14405
HGNC: HGNC:4086
Homologene: 22570
Mbtps1
Name: membrane-bound transcription factor peptidase, site 1
Synonyms: subtilisin/kexin isozyme-1, SKI-1, S1P, 0610038M03Rik, site-1 protease
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 56453
Homologene: 2808
Atm
Name: ataxia telangiectasia mutated
Synonyms: C030026E19Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 11920
HGNC: HGNC:795
Homologene: 30952
Csnk2a2
Name: casein kinase 2, alpha prime polypeptide
Synonyms: CK2, 1110035J23Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 13000
HGNC: HGNC:2459
Homologene: 20444
Eefsec
Name: eukaryotic elongation factor, selenocysteine-tRNA-specific
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 65967
Homologene: 11073
Rapgef1
Name: Rap guanine nucleotide exchange factor (GEF) 1
Synonyms: C3G, 4932418O06Rik, Grf2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 107746
HGNC: HGNC:4568
Homologene: 50501
Dtna
Name: dystrobrevin alpha
Synonyms: alpha-dystrobrevin, A0, 87K protein, Dtn, adbn, a-DB-1, 2210407P21Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 13527
VEGA: 18
HGNC: HGNC:3057
Homologene: 20362
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 153,799,872 bp
  • T to A, chromosome 1 at 160,098,278 bp
  • A to G, chromosome 2 at 29,700,732 bp
  • A to G, chromosome 2 at 52,185,281 bp
  • G to T, chromosome 2 at 65,022,620 bp
  • A to T, chromosome 2 at 73,199,498 bp
  • T to G, chromosome 2 at 85,841,114 bp
  • T to C, chromosome 2 at 152,628,097 bp
  • G to T, chromosome 4 at 58,826,453 bp
  • T to C, chromosome 4 at 107,102,492 bp
  • T to A, chromosome 4 at 130,951,966 bp
  • T to C, chromosome 4 at 150,168,427 bp
  • A to G, chromosome 5 at 70,780,713 bp
  • A to G, chromosome 5 at 115,640,088 bp
  • T to C, chromosome 5 at 143,494,603 bp
  • G to A, chromosome 5 at 150,556,889 bp
  • A to T, chromosome 6 at 88,355,629 bp
  • T to C, chromosome 6 at 89,976,259 bp
  • T to C, chromosome 7 at 30,133,590 bp
  • C to T, chromosome 7 at 81,115,563 bp
  • T to C, chromosome 7 at 98,498,541 bp
  • A to C, chromosome 8 at 4,764,905 bp
  • T to C, chromosome 8 at 16,092,305 bp
  • T to G, chromosome 8 at 48,343,406 bp
  • G to T, chromosome 8 at 70,406,189 bp
  • T to C, chromosome 8 at 79,673,896 bp
  • A to G, chromosome 8 at 95,457,469 bp
  • G to A, chromosome 8 at 105,138,174 bp
  • A to T, chromosome 8 at 119,531,091 bp
  • T to C, chromosome 9 at 21,910,519 bp
  • A to T, chromosome 9 at 25,108,988 bp
  • T to A, chromosome 9 at 38,539,144 bp
  • A to G, chromosome 9 at 53,488,587 bp
  • T to A, chromosome 10 at 7,775,333 bp
  • G to A, chromosome 10 at 119,218,010 bp
  • A to C, chromosome 11 at 73,724,560 bp
  • A to G, chromosome 11 at 98,433,249 bp
  • A to G, chromosome 11 at 117,290,800 bp
  • A to T, chromosome 12 at 31,937,247 bp
  • G to A, chromosome 12 at 81,314,350 bp
  • A to G, chromosome 13 at 8,887,505 bp
  • T to C, chromosome 13 at 119,609,231 bp
  • A to T, chromosome 14 at 24,293,380 bp
  • A to T, chromosome 14 at 49,928,662 bp
  • C to A, chromosome 14 at 103,585,136 bp
  • T to A, chromosome 15 at 54,459,807 bp
  • T to A, chromosome 15 at 80,452,715 bp
  • A to G, chromosome 16 at 32,759,308 bp
  • C to A, chromosome 16 at 36,914,671 bp
  • T to A, chromosome 17 at 35,908,564 bp
  • T to G, chromosome 18 at 5,106,724 bp
  • C to A, chromosome 18 at 23,622,056 bp
  • T to C, chromosome 18 at 31,967,990 bp
  • T to C, chromosome 18 at 41,912,465 bp
  • T to C, chromosome 19 at 7,674,282 bp
  • G to A, chromosome 19 at 38,524,751 bp
  • T to A, chromosome 19 at 61,120,425 bp
  • G to T, chromosome Y at 1,454,199 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6062 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044227-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.