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Strain Name:
C57BL/6J-MtgxR6122Btlr/Mmmh
Stock Number:
044269-MU
Citation ID:
RRID:MMRRC_044269-MU
Other Names:
R6122 (G1)
Major Collection:

Strain Information

Ap4e1
Name: adaptor-related protein complex AP-4, epsilon 1
Synonyms: 2310033A20Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 108011
HGNC: HGNC:573
Homologene: 22397
Cacna1g
Name: calcium channel, voltage-dependent, T type, alpha 1G subunit
Synonyms: a1G, Cav3.1d
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12291
HGNC: HGNC:1394
Homologene: 22544
Slc12a8
Name: solute carrier family 12 (potassium/chloride transporters), member 8
Synonyms: E330020C02Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 171286
Homologene: 11628
Itch
Name: itchy, E3 ubiquitin protein ligase
Synonyms: 6720481N21Rik, C230047C07Rik, 8030492O04Rik, AIP4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16396
Homologene: 88442
Prune1
Name: prune exopolyphosphatase
Synonyms: 9230112O05Rik, Prune-M1, Prune
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229589
Homologene: 41429
Ppp1r9a
Name: protein phosphatase 1, regulatory subunit 9A
Synonyms: 4930518N04Rik, neurabin-I, A230094E16Rik, 2810430P21Rik, Neurabin I, NRB
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243725
Homologene: 14247
Adgrg1
Name: adhesion G protein-coupled receptor G1
Synonyms: Cyt28, Gpr56
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 14766
HGNC: HGNC:4512
Homologene: 4156
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 64,735,656 bp
  • A to G, chromosome 1 at 73,952,419 bp
  • A to G, chromosome 1 at 93,497,376 bp
  • A to G, chromosome 1 at 97,183,223 bp
  • A to T, chromosome 1 at 139,248,948 bp
  • A to T, chromosome 2 at 104,256,292 bp
  • T to A, chromosome 2 at 127,028,160 bp
  • C to T, chromosome 2 at 154,143,972 bp
  • T to A, chromosome 2 at 155,174,065 bp
  • G to T, chromosome 3 at 56,029,896 bp
  • T to C, chromosome 3 at 95,262,243 bp
  • T to A, chromosome 3 at 144,926,166 bp
  • T to C, chromosome 4 at 33,161,695 bp
  • C to T, chromosome 4 at 96,444,640 bp
  • T to C, chromosome 4 at 123,039,468 bp
  • T to A, chromosome 6 at 4,905,509 bp
  • T to C, chromosome 6 at 146,952,252 bp
  • A to T, chromosome 7 at 3,842,446 bp
  • G to A, chromosome 7 at 27,459,348 bp
  • C to A, chromosome 7 at 45,819,451 bp
  • C to A, chromosome 7 at 84,361,565 bp
  • C to A, chromosome 7 at 102,954,804 bp
  • A to G, chromosome 7 at 102,955,530 bp
  • A to C, chromosome 7 at 128,085,652 bp
  • A to C, chromosome 7 at 140,943,395 bp
  • T to C, chromosome 8 at 57,526,166 bp
  • T to A, chromosome 8 at 66,883,444 bp
  • T to C, chromosome 8 at 95,002,501 bp
  • T to C, chromosome 8 at 117,082,368 bp
  • A to G, chromosome 9 at 13,266,412 bp
  • A to G, chromosome 9 at 27,063,982 bp
  • A to T, chromosome 9 at 39,593,755 bp
  • A to T, chromosome 9 at 58,917,008 bp
  • A to T, chromosome 9 at 103,346,961 bp
  • G to T, chromosome 9 at 110,387,825 bp
  • G to T, chromosome 9 at 120,131,036 bp
  • A to G, chromosome 10 at 21,058,165 bp
  • T to A, chromosome 10 at 50,617,925 bp
  • T to A, chromosome 10 at 58,465,529 bp
  • A to G, chromosome 10 at 76,506,607 bp
  • T to A, chromosome 11 at 94,430,171 bp
  • C to T, chromosome 11 at 101,468,999 bp
  • A to C, chromosome 11 at 105,994,938 bp
  • T to C, chromosome 11 at 110,070,423 bp
  • T to C, chromosome 15 at 44,557,940 bp
  • T to C, chromosome 15 at 66,828,457 bp
  • T to C, chromosome 15 at 98,860,692 bp
  • G to C, chromosome 15 at 101,815,914 bp
  • A to G, chromosome 16 at 8,605,550 bp
  • A to G, chromosome 16 at 25,422,401 bp
  • A to T, chromosome 16 at 33,625,014 bp
  • G to T, chromosome 16 at 33,985,191 bp
  • A to T, chromosome 16 at 44,217,307 bp
  • G to T, chromosome 17 at 23,820,356 bp
  • G to A, chromosome 17 at 34,934,754 bp
  • C to A, chromosome 17 at 37,589,926 bp
  • T to C, chromosome 17 at 46,521,928 bp
  • A to G, chromosome 17 at 78,813,173 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6122 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044269-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.