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Strain Name:
C57BL/6J-MtgxR6136Btlr/Mmmh
Stock Number:
044283-MU
Citation ID:
RRID:MMRRC_044283-MU
Other Names:
R6136 (G1)
Major Collection:

Strain Information

Rbm47
Name: RNA binding motif protein 47
Synonyms: 9530077J19Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 245945
Homologene: 36932
Arhgef18
Name: Rho/Rac guanine nucleotide exchange factor 18
Synonyms: D030053O22Rik, A430078G23Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 102098
Homologene: 32254
Specc1l
Name: sperm antigen with calponin homology and coiled-coil domains 1-like
Synonyms: 4930470P14Rik, 9530057A13Rik, 4932439K10Rik, Specc1l, Cytsa
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 74392
VEGA: 10
Homologene: 15610
Rap1a
Name: RAS-related protein 1a
Synonyms: Krev-1, Rap1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 109905
HGNC: HGNC:9855
Homologene: 2162
Setx
Name: senataxin
Synonyms: A930037J23Rik, Als4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 269254
HGNC: HGNC:445
Homologene: 41003
Urb2
Name: URB2 ribosome biogenesis 2 homolog (S. cerevisiae)
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 382038
Homologene: 8859
Sbno1
Name: strawberry notch 1
Synonyms: sno, 9330180L10Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 243272
Homologene: 10055
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 57,902,143 bp
  • T to G, chromosome 1 at 59,483,260 bp
  • G to A, chromosome 1 at 87,209,801 bp
  • T to C, chromosome 2 at 28,550,565 bp
  • A to G, chromosome 2 at 29,148,027 bp
  • G to T, chromosome 2 at 54,516,479 bp
  • A to G, chromosome 2 at 69,993,763 bp
  • A to G, chromosome 2 at 86,084,901 bp
  • T to A, chromosome 2 at 125,403,132 bp
  • C to A, chromosome 3 at 14,499,126 bp
  • A to G, chromosome 3 at 105,750,282 bp
  • T to C, chromosome 3 at 126,364,775 bp
  • T to C, chromosome 4 at 42,972,853 bp
  • C to A, chromosome 4 at 124,610,388 bp
  • A to G, chromosome 4 at 135,703,797 bp
  • G to A, chromosome 5 at 7,978,562 bp
  • G to C, chromosome 5 at 66,026,283 bp
  • T to C, chromosome 5 at 120,675,478 bp
  • T to C, chromosome 5 at 124,378,491 bp
  • T to C, chromosome 5 at 134,226,633 bp
  • A to G, chromosome 6 at 145,815,656 bp
  • G to A, chromosome 7 at 15,938,389 bp
  • G to T, chromosome 7 at 42,267,031 bp
  • A to C, chromosome 7 at 58,828,340 bp
  • T to G, chromosome 7 at 105,760,925 bp
  • A to T, chromosome 7 at 120,340,049 bp
  • T to A, chromosome 7 at 129,618,703 bp
  • T to C, chromosome 8 at 3,454,507 bp
  • A to G, chromosome 8 at 56,294,952 bp
  • A to G, chromosome 8 at 124,030,092 bp
  • T to A, chromosome 9 at 109,071,736 bp
  • A to G, chromosome 10 at 75,246,660 bp
  • T to A, chromosome 11 at 83,772,503 bp
  • T to C, chromosome 11 at 90,644,395 bp
  • A to G, chromosome 12 at 32,204,359 bp
  • A to T, chromosome 12 at 32,830,302 bp
  • A to G, chromosome 12 at 75,905,325 bp
  • A to G, chromosome 12 at 91,538,234 bp
  • T to C, chromosome 13 at 22,501,462 bp
  • T to C, chromosome 13 at 98,789,767 bp
  • T to C, chromosome 14 at 20,320,791 bp
  • A to G, chromosome 14 at 26,875,270 bp
  • A to T, chromosome 14 at 32,662,282 bp
  • A to T, chromosome 14 at 44,559,876 bp
  • G to A, chromosome 15 at 76,912,085 bp
  • T to C, chromosome 15 at 81,693,554 bp
  • T to C, chromosome 15 at 89,335,321 bp
  • G to T, chromosome 15 at 100,512,313 bp
  • T to A, chromosome 15 at 102,107,030 bp
  • T to G, chromosome 16 at 34,357,111 bp
  • G to A, chromosome 19 at 11,750,285 bp
  • T to A, chromosome 19 at 28,905,410 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6136 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044283-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.