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Strain Name:
C57BL/6J-MtgxR6185Btlr/Mmmh
Stock Number:
044325-MU
Citation ID:
RRID:MMRRC_044325-MU
Other Names:
R6185 (G1)
Major Collection:

Strain Information

Myo10
Name: myosin X
Synonyms: myosin-X, D15Ertd600e
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 17909
HGNC: HGNC:7593
Homologene: 36328
Epha4
Name: Eph receptor A4
Synonyms: Sek, Sek1, Tyro1, Hek8, Cek8, 2900005C20Rik, rb
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13838
HGNC: HGNC:3388
Homologene: 20933
Acadl
Name: acyl-Coenzyme A dehydrogenase, long-chain
Synonyms: LCAD, C79855
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 11363
HGNC: HGNC:88
Homologene: 37498
Syt6
Name: synaptotagmin VI
Synonyms: 3110037A08Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 54524
Homologene: 10301
Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1-n, BPAG1, Bpag1, Bpag, ah, bullous pemphigoid antigen 1, athetoid, Macf2, 2310001O04Rik, nmf203, A830042E19Rik, nmf339
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Tanc1
Name: tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 1
Synonyms: 1200003E16Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 66860
Homologene: 18946
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 34,173,080 bp
  • A to T, chromosome 1 at 36,346,018 bp
  • A to G, chromosome 1 at 66,838,363 bp
  • T to C, chromosome 1 at 77,507,106 bp
  • A to G, chromosome 1 at 116,821,273 bp
  • A to C, chromosome 1 at 128,151,861 bp
  • A to T, chromosome 1 at 150,615,438 bp
  • A to G, chromosome 1 at 164,126,346 bp
  • A to T, chromosome 2 at 4,788,522 bp
  • A to T, chromosome 2 at 30,341,710 bp
  • T to C, chromosome 2 at 33,229,014 bp
  • T to A, chromosome 2 at 59,791,585 bp
  • T to C, chromosome 2 at 69,938,277 bp
  • A to G, chromosome 2 at 87,544,743 bp
  • A to C, chromosome 3 at 55,117,219 bp
  • G to T, chromosome 3 at 79,605,807 bp
  • C to A, chromosome 3 at 96,890,833 bp
  • A to T, chromosome 3 at 103,585,528 bp
  • A to T, chromosome 3 at 122,126,140 bp
  • A to T, chromosome 4 at 11,593,804 bp
  • A to T, chromosome 4 at 53,078,089 bp
  • A to G, chromosome 4 at 89,691,768 bp
  • G to C, chromosome 4 at 96,536,086 bp
  • C to A, chromosome 4 at 100,981,031 bp
  • G to C, chromosome 4 at 129,742,275 bp
  • T to C, chromosome 4 at 129,956,449 bp
  • C to T, chromosome 4 at 141,414,514 bp
  • A to G, chromosome 4 at 150,148,993 bp
  • T to A, chromosome 5 at 43,821,552 bp
  • A to G, chromosome 5 at 73,112,788 bp
  • A to T, chromosome 5 at 74,685,608 bp
  • T to A, chromosome 5 at 92,666,854 bp
  • C to T, chromosome 5 at 115,670,153 bp
  • A to T, chromosome 5 at 149,617,695 bp
  • C to T, chromosome 6 at 28,446,348 bp
  • C to T, chromosome 6 at 83,809,970 bp
  • C to T, chromosome 7 at 15,902,880 bp
  • A to G, chromosome 7 at 29,311,271 bp
  • A to G, chromosome 7 at 42,929,011 bp
  • T to G, chromosome 7 at 44,916,170 bp
  • T to A, chromosome 7 at 63,758,385 bp
  • A to C, chromosome 7 at 99,140,832 bp
  • T to A, chromosome 7 at 104,841,424 bp
  • T to C, chromosome 7 at 128,271,177 bp
  • T to A, chromosome 7 at 142,658,381 bp
  • A to G, chromosome 8 at 14,896,544 bp
  • T to A, chromosome 8 at 35,807,510 bp
  • T to A, chromosome 8 at 53,599,147 bp
  • A to G, chromosome 8 at 91,049,137 bp
  • A to T, chromosome 8 at 125,468,253 bp
  • G to T, chromosome 9 at 18,654,473 bp
  • T to C, chromosome 9 at 39,994,124 bp
  • A to T, chromosome 9 at 120,051,378 bp
  • A to T, chromosome 10 at 79,896,363 bp
  • A to T, chromosome 11 at 4,075,244 bp
  • C to T, chromosome 11 at 16,981,829 bp
  • A to G, chromosome 11 at 32,577,749 bp
  • C to T, chromosome 11 at 78,543,562 bp
  • T to C, chromosome 11 at 102,314,023 bp
  • T to A, chromosome 11 at 105,913,039 bp
  • T to A, chromosome 12 at 82,425,028 bp
  • T to A, chromosome 13 at 4,601,253 bp
  • T to C, chromosome 14 at 52,372,562 bp
  • G to A, chromosome 14 at 69,038,364 bp
  • T to G, chromosome 15 at 25,726,510 bp
  • A to T, chromosome 15 at 74,980,030 bp
  • A to G, chromosome 15 at 89,305,611 bp
  • G to A, chromosome 15 at 98,825,714 bp
  • A to G, chromosome 15 at 99,947,649 bp
  • C to T, chromosome 16 at 10,886,209 bp
  • C to A, chromosome 16 at 21,470,141 bp
  • T to A, chromosome 16 at 32,618,272 bp
  • T to A, chromosome 16 at 55,915,092 bp
  • A to T, chromosome 17 at 17,733,382 bp
  • A to G, chromosome 17 at 25,102,040 bp
  • G to A, chromosome 17 at 34,645,329 bp
  • T to C, chromosome 17 at 84,767,024 bp
  • T to A, chromosome 17 at 90,037,136 bp
  • T to G, chromosome 18 at 20,399,486 bp
  • G to A, chromosome 18 at 73,791,128 bp
  • A to T, chromosome 19 at 7,926,588 bp
  • A to T, chromosome 19 at 44,543,284 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6185 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044325-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.