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Strain Name:
C57BL/6J-MtgxR6193Btlr/Mmmh
Stock Number:
044333-MU
Citation ID:
RRID:MMRRC_044333-MU
Other Names:
R6193 (G1)
Major Collection:

Strain Information

Rapgef2
Name: Rap guanine nucleotide exchange factor (GEF) 2
Synonyms: 5830453M24Rik, Pdzgef1, RA-GEF-1, CNRasGEF, nRapGEP
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 76089
Homologene: 35477
Ephx2
Name: epoxide hydrolase 2, cytoplasmic
Synonyms: Eph2, sEP, sEH
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 13850
VEGA: 14
HGNC: HGNC:3402
Homologene: 37558
Zfp101
Name: zinc finger protein 101
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 22643
Homologene: 137226
Cnot6
Name: CCR4-NOT transcription complex, subunit 6
Synonyms: A230103N10Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 104625
Homologene: 69187
Uaca
Name: uveal autoantigen with coiled-coil domains and ankyrin repeats
Synonyms: nucling, 2700059D02Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 72565
VEGA: 9
Homologene: 74297
Papola
Name: poly (A) polymerase alpha
Synonyms: Plap, PapIII
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 18789
Homologene: 23389
Eif2ak4
Name: eukaryotic translation initiation factor 2 alpha kinase 4
Synonyms: GCN2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 27103
Homologene: 40891
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 36,409,436 bp
  • C to A, chromosome 1 at 39,292,762 bp
  • A to T, chromosome 1 at 74,492,928 bp
  • A to G, chromosome 1 at 74,737,072 bp
  • T to A, chromosome 1 at 91,275,319 bp
  • T to C, chromosome 1 at 134,324,161 bp
  • T to C, chromosome 1 at 149,902,430 bp
  • T to G, chromosome 2 at 62,243,357 bp
  • C to T, chromosome 2 at 86,162,284 bp
  • C to T, chromosome 2 at 118,400,600 bp
  • T to C, chromosome 2 at 130,637,386 bp
  • A to T, chromosome 3 at 55,516,871 bp
  • A to T, chromosome 3 at 62,339,792 bp
  • A to T, chromosome 3 at 79,069,444 bp
  • A to G, chromosome 3 at 101,020,462 bp
  • A to G, chromosome 3 at 144,759,232 bp
  • T to A, chromosome 4 at 96,195,203 bp
  • T to A, chromosome 4 at 155,651,533 bp
  • A to G, chromosome 5 at 57,720,324 bp
  • A to G, chromosome 5 at 77,016,336 bp
  • A to T, chromosome 5 at 107,721,531 bp
  • A to T, chromosome 5 at 108,655,500 bp
  • T to A, chromosome 5 at 145,244,776 bp
  • T to A, chromosome 6 at 5,730,679 bp
  • T to A, chromosome 6 at 40,747,920 bp
  • T to G, chromosome 6 at 41,521,820 bp
  • T to A, chromosome 6 at 65,456,158 bp
  • T to C, chromosome 6 at 85,269,936 bp
  • T to A, chromosome 6 at 102,208,131 bp
  • T to C, chromosome 6 at 119,920,182 bp
  • A to T, chromosome 6 at 130,018,918 bp
  • C to G, chromosome 7 at 3,898,050 bp
  • T to G, chromosome 7 at 44,953,839 bp
  • A to G, chromosome 7 at 45,006,780 bp
  • G to A, chromosome 7 at 46,806,830 bp
  • C to A, chromosome 7 at 56,156,901 bp
  • A to G, chromosome 7 at 83,603,335 bp
  • A to T, chromosome 7 at 89,407,989 bp
  • A to G, chromosome 8 at 84,226,145 bp
  • A to G, chromosome 8 at 94,575,720 bp
  • T to A, chromosome 9 at 7,695,518 bp
  • T to C, chromosome 9 at 55,437,209 bp
  • G to A, chromosome 9 at 60,870,044 bp
  • A to C, chromosome 9 at 63,617,513 bp
  • T to C, chromosome 9 at 109,104,903 bp
  • G to A, chromosome 10 at 93,847,148 bp
  • A to G, chromosome 10 at 108,500,736 bp
  • A to T, chromosome 10 at 120,038,314 bp
  • T to C, chromosome 11 at 4,151,912 bp
  • T to A, chromosome 11 at 36,046,794 bp
  • T to A, chromosome 11 at 49,680,023 bp
  • T to A, chromosome 11 at 73,814,824 bp
  • T to C, chromosome 11 at 99,915,244 bp
  • T to A, chromosome 11 at 102,771,261 bp
  • T to A, chromosome 11 at 116,454,153 bp
  • T to G, chromosome 12 at 26,456,187 bp
  • G to T, chromosome 12 at 98,440,772 bp
  • A to T, chromosome 12 at 105,820,346 bp
  • G to A, chromosome 12 at 114,222,825 bp
  • C to A, chromosome 12 at 115,335,166 bp
  • C to T, chromosome 13 at 54,379,593 bp
  • T to G, chromosome 13 at 59,645,158 bp
  • A to G, chromosome 13 at 97,985,380 bp
  • A to G, chromosome 14 at 51,521,842 bp
  • A to T, chromosome 14 at 66,089,512 bp
  • T to G, chromosome 14 at 66,112,220 bp
  • A to G, chromosome 15 at 60,920,780 bp
  • A to G, chromosome 15 at 82,452,527 bp
  • T to C, chromosome 15 at 82,689,813 bp
  • C to T, chromosome 16 at 22,932,331 bp
  • A to T, chromosome 16 at 35,947,551 bp
  • C to A, chromosome 17 at 21,026,862 bp
  • T to C, chromosome 17 at 23,357,009 bp
  • A to C, chromosome 17 at 33,381,746 bp
  • A to C, chromosome 17 at 35,963,572 bp
  • A to G, chromosome 17 at 45,653,504 bp
  • T to G, chromosome 18 at 37,322,027 bp
  • T to C, chromosome 18 at 82,604,247 bp
  • T to A, chromosome 19 at 42,323,797 bp
  • G to T, chromosome X at 20,860,100 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6193 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044333-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.