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Strain Name:
C57BL/6J-MtgxR6199Btlr/Mmmh
Stock Number:
044339-MU
Citation ID:
RRID:MMRRC_044339-MU
Other Names:
R6199 (G1)
Major Collection:

Strain Information

Prph
Name: peripherin
Synonyms: Prph1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 19132
VEGA: 15
HGNC: HGNC:9461
Homologene: 4559
Ptprz1
Name: protein tyrosine phosphatase receptor type Z, polypeptide 1
Synonyms: DSD-1-PG, phosphacan, PTPzeta, PTPbeta, Rptpbeta, Ptpz, Ptprz, RPTPz
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 19283
HGNC: HGNC:9685
Homologene: 2136
Ank2
Name: ankyrin 2, brain
Synonyms: ankyrin B, Ank-2, Ankyrin-2, Ankyrin-B, Gm4392
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 109676
HGNC: HGNC:493
Mthfd1
Name: methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase
Synonyms: Mthfd, DCS, E430024A07Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 108156
VEGA: 12
HGNC: HGNC:7432
Homologene: 55940
Cemip2
Name: cell migration inducing hyaluronidase 2
Synonyms: 3110012M15Rik, Tmem2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 83921
VEGA: 19
Homologene: 75008
Tesk2
Name: testis-specific kinase 2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230661
Homologene: 5188
Cilk1
Name: ciliogenesis associated kinase 1
Synonyms: 2210420N10Rik, Ick
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 56542
Homologene: 69218
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 46,835,833 bp
  • T to G, chromosome 1 at 66,425,478 bp
  • TGTCCATTGGTC to TGTC, chromosome 1 at 67,162,615 bp
  • A to T, chromosome 1 at 92,491,542 bp
  • C to T, chromosome 1 at 162,682,516 bp
  • A to G, chromosome 2 at 26,469,899 bp
  • T to C, chromosome 2 at 53,157,915 bp
  • A to G, chromosome 2 at 59,978,675 bp
  • T to A, chromosome 3 at 127,004,006 bp
  • A to T, chromosome 4 at 99,978,954 bp
  • C to T, chromosome 4 at 104,731,751 bp
  • A to G, chromosome 4 at 116,792,170 bp
  • C to T, chromosome 5 at 101,872,965 bp
  • A to G, chromosome 5 at 107,433,305 bp
  • T to A, chromosome 6 at 3,376,686 bp
  • A to G, chromosome 6 at 23,002,471 bp
  • A to T, chromosome 6 at 55,379,188 bp
  • G to T, chromosome 6 at 72,430,139 bp
  • T to A, chromosome 6 at 122,047,439 bp
  • A to T, chromosome 7 at 17,714,885 bp
  • A to T, chromosome 7 at 24,465,203 bp
  • G to A, chromosome 7 at 28,835,892 bp
  • T to A, chromosome 7 at 48,389,303 bp
  • A to T, chromosome 7 at 114,791,166 bp
  • T to C, chromosome 8 at 71,525,465 bp
  • A to C, chromosome 8 at 93,217,535 bp
  • C to A, chromosome 9 at 37,804,881 bp
  • T to C, chromosome 9 at 78,164,639 bp
  • G to T, chromosome 10 at 13,506,039 bp
  • C to T, chromosome 10 at 54,014,456 bp
  • G to A, chromosome 10 at 80,702,873 bp
  • G to A, chromosome 10 at 122,920,739 bp
  • T to C, chromosome 11 at 30,891,527 bp
  • G to A, chromosome 11 at 79,020,441 bp
  • A to T, chromosome 11 at 102,840,057 bp
  • T to A, chromosome 11 at 121,831,082 bp
  • T to C, chromosome 12 at 8,298,832 bp
  • T to A, chromosome 12 at 76,288,911 bp
  • A to G, chromosome 12 at 76,303,680 bp
  • A to T, chromosome 12 at 88,441,117 bp
  • C to T, chromosome 13 at 100,300,600 bp
  • G to T, chromosome 14 at 41,153,615 bp
  • A to G, chromosome 15 at 63,825,113 bp
  • T to C, chromosome 15 at 85,787,233 bp
  • A to G, chromosome 15 at 99,056,832 bp
  • C to T, chromosome 15 at 102,108,934 bp
  • C to T, chromosome 16 at 96,421,739 bp
  • A to T, chromosome 17 at 20,462,382 bp
  • A to G, chromosome 17 at 36,671,167 bp
  • T to C, chromosome 17 at 46,649,086 bp
  • T to C, chromosome 18 at 42,578,554 bp
  • G to A, chromosome 19 at 21,844,822 bp
  • G to A, chromosome 19 at 53,383,632 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6199 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044339-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.