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Strain Name:
C57BL/6J-MtgxR6220Btlr/Mmmh
Stock Number:
044352-MU
Citation ID:
RRID:MMRRC_044352-MU
Other Names:
R6220 (G1)
Major Collection:

Strain Information

Pknox1
Name: Pbx/knotted 1 homeobox
Synonyms: PREP1, D17Wsu76e
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18771
HGNC: HGNC:9022
Homologene: 3363
Rptor
Name: regulatory associated protein of MTOR, complex 1
Synonyms: raptor, 4932417H02Rik, Rap
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 74370
Homologene: 80210
Otx1
Name: orthodenticle homeobox 1
Synonyms: A730044F23Rik, jv
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18423
HGNC: HGNC:8521
Homologene: 7875
Smarcad1
Name: SNF2 related chromatin remodeling ATPase with DExD box 1
Synonyms: D6Pas1, Etl1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 13990
Homologene: 5301
Zranb3
Name: zinc finger, RAN-binding domain containing 3
Synonyms: 4933425L19Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226409
Homologene: 32770
Armh3
Name: armadillo-like helical domain containing 3
Synonyms: 9130011E15Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 71617
VEGA: 19
Homologene: 15843
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 39,336,061 bp
  • A to C, chromosome 1 at 127,999,404 bp
  • G to A, chromosome 1 at 153,875,201 bp
  • A to T, chromosome 1 at 166,634,972 bp
  • A to T, chromosome 1 at 173,729,546 bp
  • T to C, chromosome 2 at 52,270,972 bp
  • T to A, chromosome 2 at 70,020,475 bp
  • T to C, chromosome 2 at 70,690,790 bp
  • C to T, chromosome 2 at 117,284,929 bp
  • A to G, chromosome 2 at 157,233,163 bp
  • A to G, chromosome 2 at 181,493,785 bp
  • A to G, chromosome 3 at 49,745,251 bp
  • A to T, chromosome 3 at 98,161,309 bp
  • C to T, chromosome 4 at 108,633,225 bp
  • A to T, chromosome 4 at 148,149,522 bp
  • A to T, chromosome 5 at 90,504,410 bp
  • T to C, chromosome 5 at 109,053,568 bp
  • A to G, chromosome 5 at 112,757,507 bp
  • T to A, chromosome 5 at 120,620,689 bp
  • C to A, chromosome 6 at 15,437,948 bp
  • T to A, chromosome 6 at 17,086,196 bp
  • A to G, chromosome 6 at 65,114,329 bp
  • T to A, chromosome 6 at 84,149,745 bp
  • G to A, chromosome 6 at 145,817,133 bp
  • T to C, chromosome 7 at 109,483,093 bp
  • T to A, chromosome 8 at 23,153,971 bp
  • A to G, chromosome 8 at 83,165,757 bp
  • T to C, chromosome 8 at 94,658,750 bp
  • A to G, chromosome 9 at 59,982,747 bp
  • A to G, chromosome 9 at 66,433,788 bp
  • T to A, chromosome 9 at 107,907,348 bp
  • T to A, chromosome 10 at 39,822,779 bp
  • T to A, chromosome 10 at 41,370,099 bp
  • A to T, chromosome 10 at 62,439,021 bp
  • A to G, chromosome 10 at 75,062,292 bp
  • A to T, chromosome 10 at 130,479,938 bp
  • C to A, chromosome 11 at 21,997,037 bp
  • A to G, chromosome 11 at 69,889,679 bp
  • C to A, chromosome 11 at 71,142,338 bp
  • T to C, chromosome 11 at 73,193,128 bp
  • A to T, chromosome 11 at 119,897,442 bp
  • T to C, chromosome 11 at 120,719,527 bp
  • T to A, chromosome 12 at 114,262,718 bp
  • T to G, chromosome 13 at 21,960,207 bp
  • T to C, chromosome 13 at 95,976,626 bp
  • T to C, chromosome 13 at 113,041,496 bp
  • T to C, chromosome 13 at 116,303,267 bp
  • C to A, chromosome 14 at 50,186,678 bp
  • T to C, chromosome 14 at 55,110,085 bp
  • T to A, chromosome 15 at 32,686,729 bp
  • T to C, chromosome 15 at 76,373,773 bp
  • T to A, chromosome 16 at 57,569,989 bp
  • A to T, chromosome 17 at 31,603,203 bp
  • A to T, chromosome 17 at 33,955,136 bp
  • A to T, chromosome 17 at 48,264,848 bp
  • A to G, chromosome 17 at 78,773,677 bp
  • G to C, chromosome 17 at 91,088,476 bp
  • T to G, chromosome 18 at 12,149,551 bp
  • C to A, chromosome 18 at 33,464,305 bp
  • A to G, chromosome 18 at 36,998,478 bp
  • G to A, chromosome 18 at 60,916,651 bp
  • A to T, chromosome 19 at 11,617,563 bp
  • T to C, chromosome 19 at 45,846,115 bp
  • A to T, chromosome 19 at 46,134,500 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6220 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044352-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.