Strain Name:
C57BL/6J-MtgxR6220Btlr
Stock Number:
044352-MU
Citation ID:
RRID:MMRRC_044352-MU
Other Names:
R6220 (G1)
Major Collection:

Strain Information

Pknox1
Name: Pbx/knotted 1 homeobox
Synonyms: PREP1, D17Wsu76e
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18771
HGNC: HGNC:9022
Homologene: 3363
Rptor
Name: regulatory associated protein of MTOR, complex 1
Synonyms: Rap, 4932417H02Rik, raptor
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 74370
Homologene: 80210
Otx1
Name: orthodenticle homeobox 1
Synonyms: jv, A730044F23Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18423
HGNC: HGNC:8521
Homologene: 7875
Dysf
Name: dysferlin
Synonyms: 2310004N10Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 26903
HGNC: HGNC:3097
Homologene: 20748
Smarcad1
Name: SWI/SNF-related, matrix-associated actin-dependent regulator of chromatin, subfamily a, containing DEAD/H box 1
Synonyms: Etl1, D6Pas1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 13990
Homologene: 5301
Zranb3
Name: zinc finger, RAN-binding domain containing 3
Synonyms: 4933425L19Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226409
Homologene: 32770
Armh3
Name: armadillo-like helical domain containing 3
Synonyms: 9130011E15Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 71617
VEGA: 19
Homologene: 15843
Ddx54
Name: DEAD box helicase 54
Synonyms: DEAD (Asp-Glu-Ala-Asp) box polypeptide 54, APR-5, 2410015A15Rik, DP97
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 71990
Homologene: 5590
Cc2d1b
Name: coiled-coil and C2 domain containing 1B
Synonyms: A830039B04Rik, Freud2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 319965
Homologene: 70951
Herc1
Name: HECT and RLD domain containing E3 ubiquitin protein ligase family member 1
Synonyms: 2810449H11Rik, D130015N03Rik, tbl
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235439
HGNC: HGNC:4867
Homologene: 31207
Rasgrp1
Name: RAS guanyl releasing protein 1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 19419
HGNC: HGNC:9878
Homologene: 4195
Rspry1
Name: ring finger and SPRY domain containing 1
Synonyms: 4930470D19Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 67610
Homologene: 12164
Trim66
Name: tripartite motif-containing 66
Synonyms: Tif1d, D7H11orf29
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 330627
Homologene: 28044
Jph4
Name: junctophilin 4
Synonyms: JPHL1, 9330157P13Rik, JP-4
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 319984
Homologene: 13034
Ubr3
Name: ubiquitin protein ligase E3 component n-recognin 3
Synonyms: A130030D10Rik, Zfp650, 4833421P10Rik, 1110059H15Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 68795
Homologene: 52092
Adam30
Name: a disintegrin and metallopeptidase domain 30
Synonyms: 4933424D07Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 71078
HGNC: HGNC:208
Homologene: 11038
Bcr
Name: BCR activator of RhoGEF and GTPase
Synonyms: 5133400C09Rik, breakpoint cluster region
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 110279
VEGA: 10
HGNC: HGNC:1014
Homologene: 3192
Tes
Name: testin LIM domain protein
Synonyms: testin2, Tes2, Tes1, D6Ertd352e, testin
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 21753
Homologene: 41051
Riok3
Name: RIO kinase 3
Synonyms: 1200013N13Rik, D18Ertd331e, Sudd, E130306C24Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 66878
VEGA: 18
Homologene: 2843
Gorasp2
Name: golgi reassembly stacking protein 2
Synonyms: 9430094F20Rik, 5730520M13Rik, p59, ENSMUSG00000075299, GOLPH2, GRS2, GRASP55
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 70231
Homologene: 9180
Heatr5b
Name: HEAT repeat containing 5B
Synonyms: 2010013B10Rik, A230048G03Rik, D330050P16Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 320473
VEGA: 17
Homologene: 25536
Mst1r
Name: macrophage stimulating 1 receptor (c-met-related tyrosine kinase)
Synonyms: Fv-2, Fv2, STK, CDw136, PTK8, friend virus susceptibility 2, Ron
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 19882
HGNC: HGNC:7381
Homologene: 1835
Isl1
Name: ISL1 transcription factor, LIM/homeodomain
Synonyms: Islet 1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 16392
HGNC: HGNC:6132
Homologene: 1661
Foxp2
Name: forkhead box P2
Synonyms: D0Kist7, 2810043D05Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 114142
Homologene: 134404
Arsi
Name: arylsulfatase i
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 545260
Homologene: 44428
Gm4847
Name: predicted gene 4847
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226604
Homologene: 101040
Neb
Name: nebulin
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17996
HGNC: HGNC:7720
Homologene: 136285
Elovl3
Name: ELOVL fatty acid elongase 3
Synonyms: elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 3, CIN-2, Cig30
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 12686
Homologene: 69006
Afp
Name: alpha fetoprotein
Synonyms: alpha-foetoprotein
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 11576
HGNC: HGNC:317
Homologene: 36278
Nlrp1a
Name: NLR family, pyrin domain containing 1A
Synonyms: Nalp1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 195046
Homologene: 133820
Treml4
Name: triggering receptor expressed on myeloid cells-like 4
Synonyms: 5031403H21Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224840
Mroh8
Name: maestro heat-like repeat family member 8
Synonyms: 4922505G16Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 629499
Homologene: 51864
Ifi207
Name: interferon activated gene 207
Synonyms: AI607873, Pyhin-A
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226691
HGNC: HGNC:5395
Homologene: 115929
Ak9
Name: adenylate kinase 9
Synonyms: Akd2, LOC215946, Akd1, Gm7127
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 633979
Homologene: 67934
Rev3l
Name: REV3 like, DNA directed polymerase zeta catalytic subunit
Synonyms: Sez4, Rev
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19714
HGNC: HGNC:9968
Homologene: 48147
Pcdh18
Name: protocadherin 18
Synonyms: PCDH68L
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 73173
Homologene: 10389
Supv3l1
Name: suppressor of var1, 3-like 1 (S. cerevisiae)
Synonyms: 6330443E10Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 338359
Homologene: 2386
Ctns
Name: cystinosis, nephropathic
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 83429
HGNC: HGNC:2518
Homologene: 3625
Nrxn1
Name: neurexin I
Synonyms: alpha-latrotoxin receptor (calcium-dependent), 1700062G21Rik, neurexin I alpha, 9330127H16Rik, neurexin I alpha, neurexin I beta, neurexin I beta, neurexin I alpha, neurexin I beta, A230068P09Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18189
HGNC: HGNC:8008
Homologene: 21005
Acap1
Name: ArfGAP with coiled-coil, ankyrin repeat and PH domains 1
Synonyms: Centb1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216859
Homologene: 22835
Nkx6-3
Name: NK6 homeobox 3
Synonyms: 9130417I07Rik, Nkx6.3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 74561
Homologene: 46375
Sema5a
Name: sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A
Synonyms: 9130201M22Rik, semF, M-Sema D, Semaf
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 20356
VEGA: 15
Homologene: 2949
Rassf8
Name: Ras association (RalGDS/AF-6) domain family (N-terminal) member 8
Synonyms: 5133400D11Rik, mHoj-1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 71323
Homologene: 5219
Vmn2r11
Name: vomeronasal 2, receptor 11
Synonyms: EG384219
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 384219
Homologene: 129606
Thsd4
Name: thrombospondin, type I, domain containing 4
Synonyms: B230114P05Rik, ADAMTSL6
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 207596
Homologene: 49796
Myo18b
Name: myosin XVIIIb
Synonyms: 4932408L24Rik, 4933411E19Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 74376
Homologene: 53435
Or4k2
Name: olfactory receptor family 4 subfamily K member 2
Synonyms: GA_x6K02T2PMLR-5881670-5880717, MOR247-1, Olfr730
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 258486
Homologene: 17269
Vmn2r87
Name: vomeronasal 2, receptor 87
Synonyms: EG625131
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 625131
Homologene: 129606
Gm10549
Name: predicted gene 10549
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 433171
VEGA: 18
Sv2c
Name: synaptic vesicle glycoprotein 2c
Synonyms: 4930527L09Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 75209
Homologene: 57152
Zfp184
Name: zinc finger protein 184 (Kruppel-like)
Synonyms: 4930500C15Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 193452
Homologene: 113602
Lrrc45
Name: leucine rich repeat containing 45
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217366
Homologene: 17019
Teddm1b
Name: transmembrane epididymal protein 1B
Synonyms: Gm5531
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 433365
Homologene: 131186
Fbxo6
Name: F-box protein 6
Synonyms: Fbs2, Fbxo6b, Fbx6b, FBG2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 50762
Homologene: 9274
Filip1l
Name: filamin A interacting protein 1-like
Synonyms: 4631422O05Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 78749
Homologene: 37121
Npas2
Name: neuronal PAS domain protein 2
Synonyms: bHLHe9, MOP4
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18143
HGNC: HGNC:7895
Homologene: 1887
Tssk5
Name: testis-specific serine kinase 5
Synonyms: 1700091F14Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 73542
VEGA: 15
Homologene: 18832
Rps18
Name: ribosomal protein S18
Synonyms: H2-Ke3, H-2Ke3
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 20084
Homologene: 88764
Pcdha9
Name: protocadherin alpha 9
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 192161
HGNC: HGNC:8664
Homologene: 130700
Abhd16b
Name: abhydrolase domain containing 16B
Synonyms: BC050777
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241850
Homologene: 15422
Ms4a2
Name: membrane-spanning 4-domains, subfamily A, member 2
Synonyms: Fcer1b, Fce1b, Fcrbeta, FcRB
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 14126
HGNC: HGNC:7316
Homologene: 112
Gm10735
Name: predicted gene 10735
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Ighv3-5
Name: immunoglobulin heavy variable 3-5
Synonyms: Gm7112
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 633457
Gm10645
Name: predicted gene 10645
Type: Gene
Species: Mouse
Chromosome: 8
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 39,336,061 bp
  • A to C, chromosome 1 at 127,999,404 bp
  • G to A, chromosome 1 at 153,875,201 bp
  • A to T, chromosome 1 at 166,634,972 bp
  • A to T, chromosome 1 at 173,729,546 bp
  • T to C, chromosome 2 at 52,270,972 bp
  • T to A, chromosome 2 at 70,020,475 bp
  • T to C, chromosome 2 at 70,690,790 bp
  • C to T, chromosome 2 at 117,284,929 bp
  • A to G, chromosome 2 at 157,233,163 bp
  • A to G, chromosome 2 at 181,493,785 bp
  • A to G, chromosome 3 at 49,745,251 bp
  • A to T, chromosome 3 at 98,161,309 bp
  • C to T, chromosome 4 at 108,633,225 bp
  • A to T, chromosome 4 at 148,149,522 bp
  • A to T, chromosome 5 at 90,504,410 bp
  • T to C, chromosome 5 at 109,053,568 bp
  • A to G, chromosome 5 at 112,757,507 bp
  • T to A, chromosome 5 at 120,620,689 bp
  • C to A, chromosome 6 at 15,437,948 bp
  • T to A, chromosome 6 at 17,086,196 bp
  • A to G, chromosome 6 at 65,114,329 bp
  • T to A, chromosome 6 at 84,149,745 bp
  • G to A, chromosome 6 at 145,817,133 bp
  • T to C, chromosome 7 at 109,483,093 bp
  • T to A, chromosome 8 at 23,153,971 bp
  • A to G, chromosome 8 at 83,165,757 bp
  • T to C, chromosome 8 at 94,658,750 bp
  • A to G, chromosome 9 at 59,982,747 bp
  • A to G, chromosome 9 at 66,433,788 bp
  • T to A, chromosome 9 at 107,907,348 bp
  • T to A, chromosome 10 at 39,822,779 bp
  • T to A, chromosome 10 at 41,370,099 bp
  • A to T, chromosome 10 at 62,439,021 bp
  • A to G, chromosome 10 at 75,062,292 bp
  • A to T, chromosome 10 at 130,479,938 bp
  • C to A, chromosome 11 at 21,997,037 bp
  • A to G, chromosome 11 at 69,889,679 bp
  • C to A, chromosome 11 at 71,142,338 bp
  • T to C, chromosome 11 at 73,193,128 bp
  • A to T, chromosome 11 at 119,897,442 bp
  • T to C, chromosome 11 at 120,719,527 bp
  • T to A, chromosome 12 at 114,262,718 bp
  • T to G, chromosome 13 at 21,960,207 bp
  • T to C, chromosome 13 at 95,976,626 bp
  • T to C, chromosome 13 at 113,041,496 bp
  • T to C, chromosome 13 at 116,303,267 bp
  • C to A, chromosome 14 at 50,186,678 bp
  • T to C, chromosome 14 at 55,110,085 bp
  • T to A, chromosome 15 at 32,686,729 bp
  • T to C, chromosome 15 at 76,373,773 bp
  • T to A, chromosome 16 at 57,569,989 bp
  • A to T, chromosome 17 at 31,603,203 bp
  • A to T, chromosome 17 at 33,955,136 bp
  • A to T, chromosome 17 at 48,264,848 bp
  • A to G, chromosome 17 at 78,773,677 bp
  • G to C, chromosome 17 at 91,088,476 bp
  • T to G, chromosome 18 at 12,149,551 bp
  • C to A, chromosome 18 at 33,464,305 bp
  • A to G, chromosome 18 at 36,998,478 bp
  • G to A, chromosome 18 at 60,916,651 bp
  • A to T, chromosome 19 at 11,617,563 bp
  • T to C, chromosome 19 at 45,846,115 bp
  • A to T, chromosome 19 at 46,134,500 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6220 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044352-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.