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Strain Name:
C57BL/6J-MtgxR6232Btlr/Mmmh
Stock Number:
044360-MU
Citation ID:
RRID:MMRRC_044360-MU
Other Names:
R6232 (G1)
Major Collection:

Strain Information

Myh7
Name: myosin, heavy polypeptide 7, cardiac muscle, beta
Synonyms: Myhc-b, Myhcb, beta-MHC, B-MHC, MYH-beta/slow, MyHC-I, betaMHC
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 140781
HGNC: HGNC:7577
Homologene: 68044
Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1-n, BPAG1, Bpag1, Bpag, ah, bullous pemphigoid antigen 1, athetoid, Macf2, 2310001O04Rik, nmf203, A830042E19Rik, nmf339
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Spen
Name: spen family transcription repressor
Synonyms: Mint
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 56381
Homologene: 124461
Wwp2
Name: WW domain containing E3 ubiquitin protein ligase 2
Synonyms: 1300010O06Rik, AIP2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 66894
Homologene: 48490
Nup155
Name: nucleoporin 155
Synonyms: D930027M19Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 170762
VEGA: 15
HGNC: HGNC:8063
Homologene: 43155
Arhgap39
Name: Rho GTPase activating protein 39
Synonyms: D15Wsu169e
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223666
Homologene: 27825
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 16,374,811 bp
  • T to A, chromosome 1 at 34,188,172 bp
  • T to A, chromosome 1 at 83,280,479 bp
  • T to C, chromosome 1 at 92,480,583 bp
  • T to A, chromosome 1 at 135,886,861 bp
  • T to C, chromosome 1 at 150,455,816 bp
  • G to A, chromosome 1 at 175,663,103 bp
  • C to T, chromosome 2 at 69,772,054 bp
  • T to C, chromosome 2 at 76,811,235 bp
  • G to T, chromosome 2 at 88,700,817 bp
  • G to T, chromosome 2 at 110,362,696 bp
  • G to T, chromosome 2 at 120,302,221 bp
  • A to T, chromosome 2 at 177,904,402 bp
  • G to T, chromosome 3 at 67,467,882 bp
  • A to G, chromosome 4 at 33,161,632 bp
  • C to T, chromosome 4 at 46,629,912 bp
  • A to T, chromosome 4 at 96,507,190 bp
  • A to G, chromosome 4 at 101,814,391 bp
  • C to T, chromosome 4 at 134,759,034 bp
  • T to C, chromosome 4 at 141,517,022 bp
  • T to C, chromosome 6 at 84,098,253 bp
  • T to C, chromosome 6 at 88,505,168 bp
  • G to A, chromosome 7 at 5,610,851 bp
  • A to G, chromosome 7 at 8,150,215 bp
  • T to A, chromosome 7 at 19,812,484 bp
  • A to G, chromosome 7 at 44,540,842 bp
  • A to G, chromosome 7 at 85,958,290 bp
  • A to G, chromosome 7 at 87,602,430 bp
  • ATGGCG to ATGGCGACGGTGGCG, chromosome 7 at 97,579,904 bp
  • T to A, chromosome 7 at 103,740,454 bp
  • T to G, chromosome 7 at 105,407,699 bp
  • A to T, chromosome 7 at 106,741,554 bp
  • C to T, chromosome 7 at 127,374,134 bp
  • A to T, chromosome 8 at 27,119,165 bp
  • T to G, chromosome 8 at 43,625,912 bp
  • A to G, chromosome 8 at 81,952,184 bp
  • C to A, chromosome 8 at 107,506,345 bp
  • A to T, chromosome 9 at 18,656,998 bp
  • T to G, chromosome 9 at 37,420,929 bp
  • G to A, chromosome 9 at 44,696,117 bp
  • A to G, chromosome 9 at 82,903,181 bp
  • G to T, chromosome 9 at 110,621,587 bp
  • A to G, chromosome 9 at 110,638,734 bp
  • T to A, chromosome 9 at 113,986,448 bp
  • A to T, chromosome 10 at 11,289,858 bp
  • T to A, chromosome 10 at 21,307,758 bp
  • T to A, chromosome 10 at 42,828,865 bp
  • G to A, chromosome 10 at 74,961,688 bp
  • T to A, chromosome 10 at 75,986,025 bp
  • T to C, chromosome 11 at 5,873,691 bp
  • C to T, chromosome 11 at 53,262,939 bp
  • T to A, chromosome 11 at 58,665,931 bp
  • C to A, chromosome 11 at 59,052,511 bp
  • T to A, chromosome 11 at 73,250,810 bp
  • C to G, chromosome 11 at 87,617,294 bp
  • G to A, chromosome 11 at 99,543,094 bp
  • A to T, chromosome 12 at 87,795,581 bp
  • T to C, chromosome 13 at 27,575,574 bp
  • G to A, chromosome 13 at 67,343,097 bp
  • T to A, chromosome 14 at 54,989,296 bp
  • T to G, chromosome 14 at 99,186,578 bp
  • T to C, chromosome 15 at 8,109,479 bp
  • T to C, chromosome 15 at 76,736,512 bp
  • A to G, chromosome 15 at 102,503,646 bp
  • C to T, chromosome 16 at 23,114,090 bp
  • T to C, chromosome 16 at 57,170,433 bp
  • A to C, chromosome 17 at 23,670,321 bp
  • CCACAC to CC, chromosome 17 at 32,775,520 bp
  • T to C, chromosome 17 at 46,647,430 bp
  • G to A, chromosome 19 at 13,503,063 bp
  • A to T, chromosome 19 at 44,096,992 bp
  • T to C, chromosome 19 at 55,280,501 bp
  • C to T, chromosome X at 74,277,038 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6232 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044360-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.