Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR6259Btlr/Mmmh
Stock Number:
044376-MU
Citation ID:
RRID:MMRRC_044376-MU
Other Names:
R6259 (G1)
Major Collection:

Strain Information

Map4k5
Name: mitogen-activated protein kinase kinase kinase kinase 5
Synonyms: MAPKKKK5, GCKR, KHS, 4432415E19Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 399510
HGNC: HGNC:6867
Homologene: 38199
Ank2
Name: ankyrin 2, brain
Synonyms: ankyrin B, Ank-2, Ankyrin-2, Ankyrin-B, Gm4392
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 109676
HGNC: HGNC:493
Ngf
Name: nerve growth factor
Synonyms: Ngfb
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18049
HGNC: HGNC:7808
Homologene: 1876
Cacna1h
Name: calcium channel, voltage-dependent, T type, alpha 1H subunit
Synonyms: alpha13.2, Cav3.2, T-type Cav3.2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 58226
HGNC: HGNC:1395
Homologene: 56913
Il21r
Name: interleukin 21 receptor
Synonyms: NILR
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 60504
HGNC: HGNC:6006
Homologene: 11040
Gaa
Name: glucosidase, alpha, acid
Synonyms: E430018M07Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14387
HGNC: HGNC:4065
Homologene: 37268
Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1-n, BPAG1, Bpag1, Bpag, ah, bullous pemphigoid antigen 1, athetoid, Macf2, 2310001O04Rik, nmf203, A830042E19Rik, nmf339
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 34,182,396 bp
  • T to C, chromosome 1 at 36,234,916 bp
  • C to T, chromosome 1 at 36,819,128 bp
  • C to T, chromosome 1 at 134,994,010 bp
  • T to G, chromosome 1 at 135,739,514 bp
  • T to C, chromosome 1 at 178,917,405 bp
  • A to T, chromosome 2 at 26,949,381 bp
  • A to G, chromosome 2 at 31,488,642 bp
  • T to A, chromosome 2 at 49,488,854 bp
  • T to C, chromosome 2 at 87,949,435 bp
  • T to A, chromosome 2 at 101,644,452 bp
  • C to T, chromosome 2 at 122,344,783 bp
  • G to A, chromosome 2 at 127,218,423 bp
  • T to C, chromosome 2 at 165,716,099 bp
  • A to G, chromosome 3 at 35,806,901 bp
  • A to G, chromosome 3 at 39,007,246 bp
  • A to G, chromosome 3 at 87,862,147 bp
  • A to T, chromosome 3 at 88,368,760 bp
  • A to G, chromosome 3 at 102,509,797 bp
  • T to C, chromosome 3 at 113,569,410 bp
  • T to C, chromosome 3 at 114,138,447 bp
  • A to G, chromosome 3 at 127,016,986 bp
  • T to A, chromosome 4 at 96,584,006 bp
  • C to T, chromosome 4 at 104,731,751 bp
  • T to C, chromosome 4 at 137,681,757 bp
  • A to G, chromosome 4 at 145,277,524 bp
  • T to A, chromosome 4 at 150,638,360 bp
  • A to T, chromosome 4 at 155,896,118 bp
  • A to C, chromosome 5 at 22,060,333 bp
  • A to G, chromosome 5 at 49,999,141 bp
  • C to T, chromosome 5 at 101,872,965 bp
  • T to A, chromosome 5 at 120,919,181 bp
  • A to G, chromosome 6 at 86,628,379 bp
  • A to G, chromosome 7 at 6,709,811 bp
  • G to A, chromosome 7 at 7,291,530 bp
  • C to T, chromosome 7 at 16,095,526 bp
  • A to T, chromosome 7 at 26,771,392 bp
  • T to G, chromosome 7 at 64,268,478 bp
  • A to T, chromosome 7 at 104,180,054 bp
  • A to T, chromosome 7 at 105,314,057 bp
  • T to A, chromosome 7 at 125,630,719 bp
  • C to T, chromosome 8 at 28,666,792 bp
  • TCGACG to TCG, chromosome 8 at 71,890,708 bp
  • T to A, chromosome 8 at 79,972,404 bp
  • T to C, chromosome 9 at 3,458,879 bp
  • T to C, chromosome 10 at 75,638,046 bp
  • G to T, chromosome 10 at 77,726,664 bp
  • A to G, chromosome 11 at 22,285,684 bp
  • A to G, chromosome 11 at 43,201,238 bp
  • T to A, chromosome 11 at 84,008,658 bp
  • A to T, chromosome 11 at 113,463,954 bp
  • C to T, chromosome 11 at 115,800,453 bp
  • C to T, chromosome 11 at 115,984,157 bp
  • C to A, chromosome 11 at 119,281,171 bp
  • T to C, chromosome 11 at 120,385,865 bp
  • T to A, chromosome 12 at 14,150,645 bp
  • C to A, chromosome 12 at 18,534,119 bp
  • A to G, chromosome 12 at 69,174,815 bp
  • A to G, chromosome 12 at 69,852,740 bp
  • T to C, chromosome 13 at 93,752,308 bp
  • A to G, chromosome 15 at 81,681,927 bp
  • A to G, chromosome 15 at 89,475,521 bp
  • A to T, chromosome 15 at 91,702,247 bp
  • A to T, chromosome 15 at 92,757,681 bp
  • G to T, chromosome 15 at 102,547,238 bp
  • C to T, chromosome 16 at 85,899,753 bp
  • T to A, chromosome 17 at 20,463,109 bp
  • T to C, chromosome 17 at 25,397,656 bp
  • A to T, chromosome 17 at 29,298,712 bp
  • C to T, chromosome 17 at 36,856,218 bp
  • A to G, chromosome 17 at 56,877,513 bp
  • G to A, chromosome 17 at 57,067,104 bp
  • A to T, chromosome 17 at 85,070,431 bp
  • T to A, chromosome 18 at 63,117,678 bp
  • A to G, chromosome 19 at 13,234,421 bp
  • A to C, chromosome 19 at 16,687,170 bp
  • G to T, chromosome 19 at 40,912,781 bp
  • T to A, chromosome 19 at 46,064,410 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6259 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044376-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.