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Strain Name:
C57BL/6J-MtgxR6226Btlr/Mmmh
Stock Number:
044397-MU
Citation ID:
RRID:MMRRC_044397-MU
Other Names:
R6226 (G1)
Major Collection:

Strain Information

Pitx2
Name: paired-like homeodomain transcription factor 2
Synonyms: Otlx2, Brx1b, Brx1a, Brx1, solurshin, Ptx2, Munc30, Pitx2c, Pitx2b, Pitx2a, Rieg
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18741
HGNC: HGNC:9005
Homologene: 55454
Sufu
Name: SUFU negative regulator of hedgehog signaling
Synonyms: Su(Fu), 2810026F04Rik, b2b273Clo
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 24069
Homologene: 9262
Sptbn1
Name: spectrin beta, non-erythrocytic 1
Synonyms: beta fodrin, Spnb-2, spectrin G, brain spectrin, elf3, elf1, 9930031C03Rik, non-erythrocytic, Spnb2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20742
Homologene: 2354
Nr2f2
Name: nuclear receptor subfamily 2, group F, member 2
Synonyms: COUP-TFII, ARP-1, Tcfcoup2, Aporp1, COUP-TF2, 9430015G03Rik, EAR3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 11819
HGNC: HGNC:7976
Homologene: 7628
Ptprk
Name: protein tyrosine phosphatase receptor type K
Synonyms: PTPk, RPTPkappa
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19272
VEGA: 10
HGNC: HGNC:9674
Homologene: 55693
Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1-n, BPAG1, Bpag1, Bpag, ah, bullous pemphigoid antigen 1, athetoid, Macf2, 2310001O04Rik, nmf203, A830042E19Rik, nmf339
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Kdm5b
Name: lysine demethylase 5B
Synonyms: PLU-1, 2010009J12Rik, Plu1, Rb-Bp2, 2210016I17Rik, D1Ertd202e, Jarid1b
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 75605
Homologene: 48448
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 34,270,874 bp
  • A to C, chromosome 1 at 46,126,668 bp
  • T to C, chromosome 1 at 59,265,633 bp
  • A to G, chromosome 1 at 75,196,594 bp
  • G to T, chromosome 1 at 134,608,878 bp
  • A to T, chromosome 2 at 62,777,559 bp
  • A to G, chromosome 2 at 80,508,781 bp
  • A to G, chromosome 2 at 87,252,392 bp
  • A to T, chromosome 2 at 89,666,989 bp
  • A to T, chromosome 2 at 121,441,777 bp
  • A to G, chromosome 2 at 122,088,262 bp
  • A to G, chromosome 2 at 128,650,372 bp
  • G to A, chromosome 2 at 131,055,610 bp
  • A to G, chromosome 3 at 28,806,527 bp
  • C to G, chromosome 3 at 93,398,130 bp
  • C to T, chromosome 3 at 129,215,842 bp
  • A to G, chromosome 3 at 146,584,563 bp
  • T to C, chromosome 4 at 99,657,024 bp
  • A to G, chromosome 4 at 102,966,195 bp
  • T to A, chromosome 4 at 114,845,049 bp
  • T to C, chromosome 4 at 125,659,789 bp
  • A to T, chromosome 4 at 130,055,089 bp
  • A to G, chromosome 4 at 135,965,605 bp
  • A to G, chromosome 4 at 156,173,609 bp
  • A to G, chromosome 5 at 21,217,431 bp
  • A to G, chromosome 5 at 87,092,130 bp
  • T to A, chromosome 6 at 135,240,112 bp
  • A to G, chromosome 7 at 13,035,888 bp
  • C to A, chromosome 7 at 27,351,980 bp
  • T to G, chromosome 7 at 70,359,996 bp
  • A to T, chromosome 7 at 104,734,036 bp
  • A to G, chromosome 7 at 133,862,198 bp
  • A to T, chromosome 7 at 140,812,485 bp
  • T to C, chromosome 8 at 94,286,537 bp
  • A to G, chromosome 8 at 105,689,032 bp
  • T to A, chromosome 9 at 37,746,137 bp
  • T to C, chromosome 9 at 38,816,370 bp
  • C to T, chromosome 9 at 49,565,004 bp
  • T to C, chromosome 9 at 50,607,770 bp
  • T to C, chromosome 9 at 89,091,275 bp
  • A to G, chromosome 10 at 28,564,103 bp
  • A to G, chromosome 10 at 76,515,706 bp
  • A to T, chromosome 10 at 107,771,206 bp
  • T to C, chromosome 11 at 8,901,287 bp
  • A to G, chromosome 11 at 30,136,054 bp
  • A to G, chromosome 11 at 49,148,833 bp
  • A to G, chromosome 11 at 73,140,757 bp
  • T to C, chromosome 11 at 80,821,394 bp
  • C to T, chromosome 11 at 101,468,999 bp
  • A to T, chromosome 11 at 118,277,274 bp
  • A to G, chromosome 12 at 70,863,911 bp
  • T to A, chromosome 12 at 86,748,457 bp
  • A to G, chromosome 12 at 98,680,116 bp
  • A to T, chromosome 12 at 98,715,172 bp
  • T to C, chromosome 12 at 104,101,778 bp
  • T to C, chromosome 13 at 12,278,967 bp
  • A to T, chromosome 13 at 18,129,154 bp
  • T to C, chromosome 13 at 21,180,916 bp
  • T to A, chromosome 13 at 61,568,721 bp
  • A to G, chromosome 14 at 99,101,119 bp
  • G to A, chromosome 16 at 5,176,565 bp
  • A to T, chromosome 16 at 58,731,300 bp
  • T to A, chromosome 17 at 25,320,539 bp
  • G to A, chromosome 17 at 28,057,330 bp
  • A to T, chromosome 17 at 36,121,083 bp
  • T to A, chromosome 18 at 20,579,449 bp
  • A to G, chromosome 19 at 28,317,302 bp
  • G to T, chromosome 19 at 32,701,587 bp
  • G to A, chromosome 19 at 46,473,654 bp
  • T to A, chromosome 19 at 50,181,414 bp
  • T to C, chromosome 19 at 56,916,128 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6226 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044397-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.