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Strain Name:
C57BL/6J-MtgxR6270Btlr/Mmmh
Stock Number:
044441-MU
Citation ID:
RRID:MMRRC_044441-MU
Other Names:
R6270 (G1)
Major Collection:

Strain Information

Kit
Name: KIT proto-oncogene receptor tyrosine kinase
Synonyms: Steel Factor Receptor, c-KIT, Dominant white spotting, belly-spot, Tr-kit, SOW3, SCO5, SCO1, Gsfsow3, Gsfsco5, Gsfsco1, CD117
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 16590
HGNC: HGNC:6342
Homologene: 187
Otx1
Name: orthodenticle homeobox 1
Synonyms: A730044F23Rik, jv
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18423
HGNC: HGNC:8521
Homologene: 7875
Ark2n
Name: arkadia (RNF111) N-terminal like PKA signaling regulator 2N
Synonyms: Ark2N, 8030462N17Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 212163
VEGA: 18
Homologene: 17035
Sf3b3
Name: splicing factor 3b, subunit 3
Synonyms: 5730409A01Rik, 1810061H24Rik, SAP130, RSE1, D8Ertd633e
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 101943
Homologene: 6579
Gse1
Name: genetic suppressor element 1, coiled-coil protein
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 382034
Homologene: 40964
Gphn
Name: gephyrin
Synonyms: geph, 5730552E08Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 268566
VEGA: 12
Homologene: 10820
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 71,637,275 bp
  • T to G, chromosome 1 at 169,988,540 bp
  • C to A, chromosome 2 at 30,392,269 bp
  • A to G, chromosome 2 at 32,673,643 bp
  • T to C, chromosome 2 at 62,547,788 bp
  • T to C, chromosome 2 at 90,757,463 bp
  • C to A, chromosome 3 at 33,800,388 bp
  • A to T, chromosome 5 at 12,448,107 bp
  • T to A, chromosome 5 at 23,273,351 bp
  • A to G, chromosome 5 at 75,609,509 bp
  • A to T, chromosome 5 at 87,517,554 bp
  • T to A, chromosome 6 at 41,674,359 bp
  • T to C, chromosome 6 at 42,593,791 bp
  • T to C, chromosome 6 at 83,375,791 bp
  • C to A, chromosome 7 at 62,380,658 bp
  • T to C, chromosome 7 at 103,051,331 bp
  • T to C, chromosome 7 at 103,660,413 bp
  • T to C, chromosome 8 at 13,018,701 bp
  • G to A, chromosome 8 at 48,367,394 bp
  • C to T, chromosome 8 at 84,230,951 bp
  • T to C, chromosome 8 at 110,841,820 bp
  • T to C, chromosome 8 at 120,569,163 bp
  • G to A, chromosome 9 at 35,561,184 bp
  • A to T, chromosome 10 at 79,194,325 bp
  • A to G, chromosome 10 at 79,293,815 bp
  • C to A, chromosome 11 at 21,997,037 bp
  • A to G, chromosome 11 at 54,352,107 bp
  • A to G, chromosome 11 at 86,799,605 bp
  • C to A, chromosome 11 at 100,247,203 bp
  • G to A, chromosome 12 at 13,324,293 bp
  • T to G, chromosome 12 at 78,522,950 bp
  • T to C, chromosome 12 at 99,388,417 bp
  • A to G, chromosome 12 at 105,768,701 bp
  • C to T, chromosome 13 at 3,581,891 bp
  • T to C, chromosome 13 at 92,688,662 bp
  • A to T, chromosome 14 at 33,009,121 bp
  • A to G, chromosome 14 at 53,649,866 bp
  • T to C, chromosome 14 at 70,579,390 bp
  • T to C, chromosome 14 at 78,518,799 bp
  • G to T, chromosome 15 at 85,821,105 bp
  • AGGG to AGGGG, chromosome 15 at 97,808,495 bp
  • A to G, chromosome 15 at 101,419,558 bp
  • A to T, chromosome 16 at 58,824,419 bp
  • G to A, chromosome 17 at 28,881,368 bp
  • T to C, chromosome 17 at 70,844,866 bp
  • A to T, chromosome 18 at 37,436,785 bp
  • A to G, chromosome 18 at 52,638,364 bp
  • T to C, chromosome 18 at 77,674,421 bp
  • C to T, chromosome 19 at 6,914,120 bp
  • T to C, chromosome 19 at 10,674,861 bp
  • T to C, chromosome 19 at 38,158,436 bp
  • A to G, chromosome 19 at 56,306,863 bp
  • C to T, chromosome 19 at 56,320,198 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6270 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044441-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.